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Previous <jats:italic>in\u00a0vitro<\/jats:italic> work had pointed to a role of the zinc finger containing specificity proteins Sp1 and Sp3 as major regulators of glial differentiation and myelination. Here, we asked whether such a role is also evident <jats:italic>in\u00a0vivo<\/jats:italic> using mice with specific deletions of Sp1 or Sp3 in myelinating glia. We also studied glia\u2010specific conditional Sp2\u2010 and constitutive Sp4\u2010deficient mice to include all related glutamine\u2010rich Sp\u00a0factors into our analysis. Surprisingly, we did not detect\u00a0developmental Schwann cell abnormalities in any of\u00a0the mutant mice. Oligodendrocyte development and differentiation was also not fundamentally affected as oligodendrocytes were present in all mouse mutants and retained their ability to differentiate and initiate myelin gene expression. The most severe defect we observed was a 50% reduction in Mbp\u2010 and proteolipid protein 1 (Plp1)\u2010positive differentiating oligodendrocytes in Sp2 mutants at birth. Unexpectedly, glial development appeared undisturbed even in the joint absence of Sp1 and Sp3. We conclude that Sp2 has a minor effect on the differentiation of myelinating glia, and that glutamine\u2010rich Sp proteins are not essential regulators of the process.<\/jats:p><\/jats:sec><jats:sec><jats:label \/><jats:p>\n<jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc13908-fig-0008-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text>\n<\/jats:p><\/jats:sec>","DOI":"10.1111\/jnc.13908","type":"journal-article","created":{"date-parts":[[2016,11,27]],"date-time":"2016-11-27T09:06:41Z","timestamp":1480237601000},"page":"245-256","source":"Crossref","is-referenced-by-count":3,"title":["Sp2 is the only glutamine\u2010rich specificity protein with minor impact on development and differentiation in myelinating glia"],"prefix":"10.1111","volume":"140","author":[{"given":"Am\u00e9lie","family":"Wegener","sequence":"first","affiliation":[{"name":"Institut f\u00fcr Biochemie Emil\u2010Fischer\u2010Zentrum Friedrich\u2010Alexander Universit\u00e4t Erlangen\u2010N\u00fcrnberg Erlangen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melanie","family":"K\u00fcspert","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Biochemie Emil\u2010Fischer\u2010Zentrum Friedrich\u2010Alexander Universit\u00e4t Erlangen\u2010N\u00fcrnberg Erlangen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisabeth","family":"Sock","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Biochemie Emil\u2010Fischer\u2010Zentrum Friedrich\u2010Alexander Universit\u00e4t Erlangen\u2010N\u00fcrnberg Erlangen 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