{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T06:29:34Z","timestamp":1789972174629,"version":"4.0.1"},"reference-count":38,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,4,12]],"date-time":"2017-04-12T00:00:00Z","timestamp":1491955200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005627","name":"Universit\u00e9 de Li\u00e8ge","doi-asserted-by":"publisher","award":["13\/17\u201007"],"award-info":[{"award-number":["13\/17\u201007"]}],"id":[{"id":"10.13039\/501100005627","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Because of the progressive loss of nigro\u2010striatal dopaminergic terminals in Parkinson's disease (<jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content>), <jats:italic>in\u00a0vivo<\/jats:italic> quantitative imaging of dopamine (<jats:styled-content style=\"fixed-case\">DA<\/jats:styled-content>) containing neurons in animal models of <jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content> is of critical importance in the preclinical evaluation of highly awaited disease\u2010modifying therapies. Among existing methods, the high sensitivity of positron emission tomography (<jats:styled-content style=\"fixed-case\">PET<\/jats:styled-content>) is attractive to achieve that goal. The aim of this study was to perform a quantitative comparison of brain images obtained in 6\u2010hydroxydopamine (6\u2010<jats:styled-content style=\"fixed-case\">OHDA<\/jats:styled-content>) lesioned rats using two dopaminergic <jats:styled-content style=\"fixed-case\">PET<\/jats:styled-content> radiotracers, namely [<jats:sup>18<\/jats:sup>F]fluoro\u20103,4\u2010dihydroxyphenyl\u2010<jats:italic>L<\/jats:italic>\u2010alanine ([<jats:sup>18<\/jats:sup>F]<jats:styled-content style=\"fixed-case\">FDOPA<\/jats:styled-content>) and 6\u2010[<jats:sup>18<\/jats:sup>F]fluoro\u2010<jats:italic>L<\/jats:italic>\u2010m\u2010tyrosine ([<jats:sup>18<\/jats:sup>F]<jats:styled-content style=\"fixed-case\">FMT<\/jats:styled-content>). Because the imaging signal is theoretically less contaminated by metabolites, we hypothesized that the latter would show stronger relationship with behavioural and post\u2010mortem measures of striatal dopaminergic deficiency. We used a within\u2010subject design to measure striatal [<jats:sup>18<\/jats:sup>F]<jats:styled-content style=\"fixed-case\">FMT<\/jats:styled-content> and [<jats:sup>18<\/jats:sup>F]<jats:styled-content style=\"fixed-case\">FDOPA<\/jats:styled-content> uptake in eight partially lesioned, eight fully lesioned and ten sham\u2010treated rats. Animals were pretreated with an <jats:italic>L<\/jats:italic>\u2010aromatic amino acid decarboxylase inhibitor. A catechol\u2010<jats:italic>O<\/jats:italic>\u2010methyl transferase inhibitor was also given before [<jats:sup>18<\/jats:sup>F]<jats:styled-content style=\"fixed-case\">FDOPA PET<\/jats:styled-content>. Quantitative estimates of striatal uptake were computed using conventional graphical Patlak method. Striatal dopaminergic deficiencies were measured with apomorphine\u2010induced rotations and post\u2010mortem striatal <jats:styled-content style=\"fixed-case\">DA<\/jats:styled-content> content. We observed a strong relationship between [<jats:sup>18<\/jats:sup>F]<jats:styled-content style=\"fixed-case\">FMT<\/jats:styled-content> and [<jats:sup>18<\/jats:sup>F]<jats:styled-content style=\"fixed-case\">FDOPA<\/jats:styled-content> estimates of decreased uptake in the denervated striatum using the tissue\u2010derived uptake rate constant <jats:italic>K<\/jats:italic><jats:sub>c<\/jats:sub>. However, only [<jats:sup>18<\/jats:sup>F]<jats:styled-content style=\"fixed-case\">FMT <\/jats:styled-content><jats:italic>K<\/jats:italic><jats:sub>c<\/jats:sub> succeeded to discriminate between the partial and the full 6\u2010<jats:styled-content style=\"fixed-case\">OHDA<\/jats:styled-content> lesion and correlated well with the post\u2010mortem striatal <jats:styled-content style=\"fixed-case\">DA<\/jats:styled-content> content. This study indicates that the [<jats:sup>18<\/jats:sup>F]<jats:styled-content style=\"fixed-case\">FMT<\/jats:styled-content> could be more sensitive, with respect of [<jats:sup>18<\/jats:sup>F]<jats:styled-content style=\"fixed-case\">FDOPA<\/jats:styled-content>, to investigate <jats:styled-content style=\"fixed-case\">DA<\/jats:styled-content> terminals loss in 6\u2010<jats:styled-content style=\"fixed-case\">OHDA<\/jats:styled-content> rats, and open the way to <jats:italic>in\u00a0vivo L<\/jats:italic>\u2010aromatic amino acid decarboxylase activity targeting in future investigations on progressive <jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content> models.<\/jats:p><\/jats:sec><jats:sec><jats:label\/><jats:p>\n<jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc14016-fig-0007-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text>\n<\/jats:p><\/jats:sec>","DOI":"10.1111\/jnc.14016","type":"journal-article","created":{"date-parts":[[2017,3,10]],"date-time":"2017-03-10T23:06:48Z","timestamp":1489187208000},"page":"626-635","source":"Crossref","is-referenced-by-count":21,"title":["Comparative assessment of 6\u2010[<sup>18<\/sup>F]fluoro\u2010<i>L<\/i>\u2010m\u2010tyrosine and 6\u2010[<sup>18<\/sup>F]fluoro\u2010<i>L<\/i>\u2010dopa to evaluate dopaminergic presynaptic integrity in a Parkinson's disease rat model"],"prefix":"10.1111","volume":"141","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1714-0267","authenticated-orcid":false,"given":"Guillaume","family":"Becker","sequence":"first","affiliation":[{"name":"GIGA \u2010 CRC In vivo Imaging University of Li\u00e8ge Li\u00e8ge Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed Ali","family":"Bahri","sequence":"additional","affiliation":[{"name":"GIGA \u2010 CRC In vivo Imaging University of Li\u00e8ge Li\u00e8ge Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Michel","sequence":"additional","affiliation":[{"name":"Neurosciences TA Biology UCB Pharma Braine L'Alleud Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabian","family":"Hustadt","sequence":"additional","affiliation":[{"name":"Neurosciences TA Biology UCB Pharma Braine L'Alleud Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ga\u00ebtan","family":"Garraux","sequence":"additional","affiliation":[{"name":"GIGA \u2010 CRC In vivo Imaging University of Li\u00e8ge Li\u00e8ge Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9","family":"Luxen","sequence":"additional","affiliation":[{"name":"GIGA \u2010 CRC In vivo Imaging University of Li\u00e8ge Li\u00e8ge Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Lemaire","sequence":"additional","affiliation":[{"name":"GIGA \u2010 CRC In vivo Imaging University of Li\u00e8ge Li\u00e8ge Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alain","family":"Plenevaux","sequence":"additional","affiliation":[{"name":"GIGA \u2010 CRC In vivo Imaging University of Li\u00e8ge Li\u00e8ge Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,4,12]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.2967\/jnumed.109.063974"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/00004647-199607000-00018"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(88)90732-2"},{"key":"e_1_2_9_5_1","first-page":"630","article-title":"Evaluation of fluorinated m\u2010tyrosine analogs as PET imaging agents of dopamine nerve terminals: comparison with 6\u2010fluoroDOPA","volume":"38","author":"DeJesus O. T.","year":"1997","journal-title":"J. Nucl. Med."},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.2002.7891"},{"key":"e_1_2_9_7_1","first-page":"209","article-title":"Combined FDOPA and 3OMFD PET studies in Parkinson's disease","volume":"37","author":"Dhawan V.","year":"1996","journal-title":"J. Nucl. Med."},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(09)70238-8"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.1991.129"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/00004647-199903000-00006"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2011.01426.x"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0024-3205(97)00300-7"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/jnc.13162"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/mds.23778"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/305137a0"},{"key":"e_1_2_9_16_1","first-page":"1568","article-title":"Graphical analysis of 6\u2010fluoro\u2010L\u2010dopa trapping: effect of inhibition of catechol\u2010O\u2010methyltransferase","volume":"38","author":"Holden J. E.","year":"1997","journal-title":"J. Nucl. Med."},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(93)90576-9"},{"issue":"2","key":"e_1_2_9_18_1","first-page":"S122","article-title":"Future of cell and gene therapies for Parkinson's disease","volume":"64","author":"Isacson O.","year":"2008","journal-title":"Ann. Neurol."},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp.66.6.754"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.2967\/jnumed.115.161802"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/2191-219X-1-25"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/jlcr.3291"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1745-3984.1975.tb01012.x"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.2967\/jnumed.112.112284"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.1991.154"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0114086"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/mds.870100312"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(13)61939-X"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410340306"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.1985.87"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200005183422004"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1027\/0044-3409.217.1.6"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.44.7.1292"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-0082(96)00040-8"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410340304"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1037\/1082-989X.9.2.164"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(14)60041-6"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(70)90187-3"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.2012.120"}],"container-title":["Journal of Neurochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjnc.14016","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jnc.14016","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T08:42:52Z","timestamp":1696236172000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jnc.14016"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,4,12]]},"references-count":38,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2017,5]]}},"alternative-id":["10.1111\/jnc.14016"],"URL":"https:\/\/doi.org\/10.1111\/jnc.14016","archive":["Portico"],"relation":{},"ISSN":["0022-3042","1471-4159"],"issn-type":[{"value":"0022-3042","type":"print"},{"value":"1471-4159","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,4,12]]}}}