{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T04:56:57Z","timestamp":1790312217640,"version":"4.1.0"},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,9,5]],"date-time":"2017-09-05T00:00:00Z","timestamp":1504569600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Fondation France Parkinson"},{"name":"R\u00e9gion Auvergne-Rh\u00f4ne-Alpes - ARC1 Sant\u00e9"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2017,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>The M83 transgenic mouse is a model of human synucleinopathies that develops severe motor impairment correlated with accumulation of the pathological Ser129\u2010phosphorylated \u03b1\u2010synuclein (\u03b1\u2010syn<jats:sup>P<\/jats:sup>) in the brain and spinal cord. M83 disease can be accelerated by intracerebral inoculation of brain extracts from sick M83 mice. This has also recently been described using peripheral routes, injecting recombinant preformed \u03b1\u2010syn fibrils into the muscle or the peritoneum. Here, we inoculated homozygous and\/or hemizygous M83 neonates via the intraperitoneal and\/or intracerebral routes with two different brain extracts: one from sick M83 mice inoculated with brain extract from other sick M83 mice, and the other derived from a human multiple system atrophy source passaged in M83 mice. Detection of \u03b1\u2010syn<jats:sup>P<\/jats:sup> using <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> and western blot confirmed the disease in mice. The distribution of \u03b1\u2010syn<jats:sup>P<\/jats:sup> in the central nervous system was similar, independently of the inoculum or inoculation route, consistent with previous studies describing M83 disease. <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> tests revealed higher levels of \u03b1\u2010syn<jats:sup>P<\/jats:sup> in homozygous than in hemizygous sick M83 mice, at least after <jats:styled-content style=\"fixed-case\">IC<\/jats:styled-content> inoculation. Interestingly, the immunoreactivity of \u03b1\u2010syn<jats:sup>P<\/jats:sup> detected by <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> was significantly lower in M83 mice inoculated with the multiple system atrophy inoculum than in M83 mice inoculated with the M83 inoculum, at the first two passages. \u2018Prion\u2010like\u2019 propagation of the synucleinopathy up to the clinical disease was accelerated by both intracerebral and intraperitoneal inoculations of brain extracts from sick mice. This acceleration, however, depends on the levels of \u03b1\u2010syn expression by the mouse and the type of inoculum.<\/jats:p><\/jats:sec><jats:sec><jats:label\/><jats:p>\n<jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc14139-fig-0005-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text>\n<\/jats:p><\/jats:sec>","DOI":"10.1111\/jnc.14139","type":"journal-article","created":{"date-parts":[[2017,8,3]],"date-time":"2017-08-03T20:29:09Z","timestamp":1501792149000},"page":"126-135","source":"Crossref","is-referenced-by-count":32,"title":["\u2018Prion\u2010like\u2019 propagation of the synucleinopathy of M83 transgenic mice depends on the mouse genotype and type of inoculum"],"prefix":"10.1111","volume":"143","author":[{"given":"Dorian","family":"Sargent","sequence":"first","affiliation":[{"name":"Agence Nationale de S\u00e9curit\u00e9 Sanitaire de l'alimentation de l'environnement et du travail Universit\u00e9 de Lyon Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9r\u00e9my","family":"Verch\u00e8re","sequence":"additional","affiliation":[{"name":"Agence Nationale de S\u00e9curit\u00e9 Sanitaire de l'alimentation de l'environnement et du travail Universit\u00e9 de Lyon Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corinne","family":"Lazizzera","sequence":"additional","affiliation":[{"name":"Agence Nationale de S\u00e9curit\u00e9 Sanitaire de l'alimentation de l'environnement et du travail Universit\u00e9 de Lyon Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Damien","family":"Gaillard","sequence":"additional","affiliation":[{"name":"Agence Nationale de S\u00e9curit\u00e9 Sanitaire de l'alimentation de l'environnement et du travail Universit\u00e9 de Lyon Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Latifa","family":"Lakhdar","sequence":"additional","affiliation":[{"name":"Agence Nationale de S\u00e9curit\u00e9 Sanitaire de l'alimentation de l'environnement et du travail Universit\u00e9 de Lyon Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Streichenberger","sequence":"additional","affiliation":[{"name":"Centre de Pathologie et Neuropathologie Est Hospices Civils de Lyon Universit\u00e9 de Lyon Institut Neuromyog\u00e8ne CNRS INSERM Bron France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Morignat","sequence":"additional","affiliation":[{"name":"Agence Nationale de S\u00e9curit\u00e9 Sanitaire de l'alimentation de l'environnement et du travail Universit\u00e9 de Lyon Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"B\u00e9temps","sequence":"additional","affiliation":[{"name":"Agence Nationale de S\u00e9curit\u00e9 Sanitaire de l'alimentation de l'environnement et du travail Universit\u00e9 de Lyon Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5396-7707","authenticated-orcid":false,"given":"Thierry","family":"Baron","sequence":"additional","affiliation":[{"name":"Agence Nationale de S\u00e9curit\u00e9 Sanitaire de l'alimentation de l'environnement et du travail Universit\u00e9 de Lyon Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,9,5]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"1","article-title":"Mapping of surface\u2010exposed epitopes of in\u00a0vitro and in\u00a0vivo aggregated species of alpha\u2010synuclein","volume":"1","author":"Almandoz\u2010Gil L.","year":"2016","journal-title":"Cell. 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