{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T15:07:12Z","timestamp":1787843232364,"version":"build-2784847793"},"reference-count":48,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,11,6]],"date-time":"2017-11-06T00:00:00Z","timestamp":1509926400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>The <jats:italic>olfm1a and olfm1b<\/jats:italic> genes in zebrafish encode conserved secreted glycoproteins. These genes are preferentially expressed in the brain and retina starting from 16\u00a0h post\u2010fertilization until adulthood. Functions of the <jats:italic>Olfm1<\/jats:italic> gene is still unclear. Here, we produced and analyzed a null zebrafish mutant of both <jats:italic>olfm1a<\/jats:italic> and <jats:italic>olfm1b<\/jats:italic> genes (<jats:italic>olfm1<\/jats:italic> null). <jats:italic>olfm1<\/jats:italic> null fish were born at a normal Mendelian ratio and showed normal body shape and fertility as well as no visible defects from larval stages to adult. Olfm1 proteins were preferentially localized in the synaptosomes of the adult brain. Olfm1 co\u2010immunoprecipitated with GluR2 and soluble NSF attachment protein receptor complexes indicating participation of Olfm1 in both pre\u2010 and post\u2010synaptic events. Phosphorylation of GluR2 was not changed while palmitoylation of GluR2 was decreased in the brain synaptosomal membrane fraction of <jats:italic>olfm1<\/jats:italic> null compared with wt fish. The levels of GluR2, <jats:styled-content style=\"fixed-case\">SNAP<\/jats:styled-content>25, flotillin1, and <jats:styled-content style=\"fixed-case\">VAMP<\/jats:styled-content>2 were markedly reduced in the synaptic microdomain of <jats:italic>olfm1<\/jats:italic> null brain compared with wt. The internalization of GluR2 in retinal cells and the localization of <jats:styled-content style=\"fixed-case\">VAMP<\/jats:styled-content>2 in brain synaptosome were modified by <jats:italic>olfm1<\/jats:italic> null mutation. This indicates that Olfm1 may regulate receptor trafficking from the intracellular compartments to the synaptic membrane microdomain, partly through the alteration of post\u2010translational GluR2 modifications such as palmitoylation. Olfm1 may be considered a novel regulator of the composition and function of the \u03b1\u2010amino\u20103\u2010hydroxy\u20105\u2010methylisoxazole\u20104\u2010propionate receptor complex.<\/jats:p><\/jats:sec><jats:sec><jats:label\/><jats:p>\n<jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc14231-fig-0005-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text>\n<\/jats:p><\/jats:sec>","DOI":"10.1111\/jnc.14231","type":"journal-article","created":{"date-parts":[[2017,10,4]],"date-time":"2017-10-04T10:14:38Z","timestamp":1507112078000},"page":"635-644","source":"Crossref","is-referenced-by-count":14,"title":["Impaired <scp>AMPA<\/scp> receptor trafficking by a double knockout of zebrafish <i>olfactomedin1a\/b<\/i>"],"prefix":"10.1111","volume":"143","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2200-5200","authenticated-orcid":false,"given":"Naoki","family":"Nakaya","sequence":"first","affiliation":[{"name":"Section\u00a0of Retinal Ganglion Cell Biology Laboratory of Retinal Cell and Molecular Biology National Eye Institute, NIH Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Afia","family":"Sultana","sequence":"additional","affiliation":[{"name":"Section\u00a0of Retinal Ganglion Cell Biology Laboratory of Retinal Cell and Molecular Biology National Eye Institute, NIH Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stanislav I.","family":"Tomarev","sequence":"additional","affiliation":[{"name":"Section\u00a0of Retinal Ganglion Cell Biology Laboratory of Retinal Cell and Molecular Biology National Eye Institute, NIH Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,11,6]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"36","article-title":"Image processing with ImageJ","volume":"11","author":"Abramoff M. 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