{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T08:06:26Z","timestamp":1767773186687,"version":"build-2238731810"},"reference-count":82,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,12,4]],"date-time":"2017-12-04T00:00:00Z","timestamp":1512345600000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001407","name":"Department of Biotechnology , Ministry of Science and Technology","doi-asserted-by":"publisher","award":["BT\/PR10400\/MED\/31\/251\/2013"],"award-info":[{"award-number":["BT\/PR10400\/MED\/31\/251\/2013"]}],"id":[{"id":"10.13039\/501100001407","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2017,12]]},"abstract":"Abstract<\/jats:title>\n \n \n \n Stroke is a multi\u2010factorial polygenic disease and is a major cause of death and adult disability. Administration of bone marrow stem cells protects ischemic rat brain by facilitating recovery of neurological functions. But the molecular mechanism of stem cells action and their effect on gene expression is not well explored. In this study, we have transplanted 1\u00a0\u00d7\u00a010\n 6<\/jats:sup>\n human bone marrow mesenchymal stem cells (\n hBMMSC<\/jats:styled-content>\n s) in middle cerebral artery occluded (\n MCA<\/jats:styled-content>\n o) adult male Wistar rats through intracarotid artery route at 24\u00a0h after surgery. Motor behavioral tests (rotarod and open field) were performed to assess the changes in motor functions at day 0 and day1, 4, 8 and 14. The expression of studied genes at\n mRNA<\/jats:styled-content>\n and protein level was quantified by using Q\u2010\n PCR<\/jats:styled-content>\n and western blotting, respectively. Further, we have assessed the methylation pattern of promoter of these genes by using methylation\u2010specific\n PCR<\/jats:styled-content>\n . Data were analyzed statistically and correlated. A significant improvement in behavioral deficits was observed in stem cells treated group after 14th day post stroke. Significantly (\n p<\/jats:italic>\n \u00a0<\u00a00.05) increased\n mRNA<\/jats:styled-content>\n and protein levels of brain derived neurotrophic factor and\n ANP<\/jats:styled-content>\n genes in\n hBMMSC<\/jats:styled-content>\n s treated group along with decrease in methylation level at their promoter was observed. On the other hand, significantly decreased\n mRNA<\/jats:styled-content>\n and protein level of\n TSP<\/jats:styled-content>\n 1 and\n WNK<\/jats:styled-content>\n 1 in\n hBMMSC<\/jats:styled-content>\n s treated group was observed. In conclusion,\n hBMMSC<\/jats:styled-content>\n s administration significantly improves the behavioral deficits by improving motor and locomotor coordination. The promoter of\n TSP<\/jats:styled-content>\n 1 and\n WNK<\/jats:styled-content>\n 1 genes was found to be hyper\u2010methylated in\n hBMMSC<\/jats:styled-content>\n s group resulting in their decreased expression while the promoter of\n ANP<\/jats:styled-content>\n and brain derived neurotrophic factor was found to be hypo\u2010methylated. This study might shed a light on how\n hBMMSC<\/jats:styled-content>\n s affect the gene expression by modulating methylation status.\n <\/jats:p>\n <\/jats:sec>\n \n \n \n \n \n image<\/jats:alt-text>\n <\/jats:graphic>\n <\/jats:boxed-text>\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/jnc.14241","type":"journal-article","created":{"date-parts":[[2017,10,19]],"date-time":"2017-10-19T12:26:40Z","timestamp":1508416000000},"page":"722-735","source":"Crossref","is-referenced-by-count":4,"title":["Retracted: Intra\u2010arterial transplantation of human bone marrow mesenchymal stem cells (\n hBMMSC<\/scp>\n s) improves behavioral deficits and alters gene expression in rodent stroke model"],"prefix":"10.1111","volume":"143","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0801-8013","authenticated-orcid":false,"family":"Vibhuti","sequence":"first","affiliation":[{"name":"Department of Neurology All India Institute of Medical Sciences (AIIMS) New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rehan","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Biochemistry All India Institute of Medical Sciences (AIIMS) New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alpana","family":"Sharma","sequence":"additional","affiliation":[{"name":"Department of Biochemistry All India Institute of Medical Sciences (AIIMS) New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suman","family":"Jain","sequence":"additional","affiliation":[{"name":"Department of Physiology All India Institute of Medical Sciences (AIIMS) New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sujata","family":"Mohanty","sequence":"additional","affiliation":[{"name":"Stem Cell Facility All India Institute of Medical Sciences (AIIMS) New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9673-2473","authenticated-orcid":false,"given":"Kameshwar","family":"Prasad","sequence":"additional","affiliation":[{"name":"Department of Neurology All India Institute of Medical Sciences (AIIMS) New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,12,4]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2004.01.004"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1159\/000333381"},{"key":"e_1_2_8_4_1","first-page":"43","article-title":"Association between single nucleotide polymorphisms in Wnk1 gene and ischemic stroke in Chinese Han population","volume":"43","author":"Cai J.","year":"2014","journal-title":"Zhejiang. 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