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The purpose of this study was to investigate whether Sol andTA MN<\/jats:styled-content>s adapt to changes in cholinergic environment by differential regulation of their muscarinic receptors M2 (M2R). We examinedChrm2<\/jats:italic>(M2R gene) transcript level, high\u2010affinity 3\u2010quinuclidinyl benzilate\u20103<\/jats:sup>H ([3<\/jats:sup>H]QNB<\/jats:styled-content>) ligand binding, distribution and density of M2R immunolabeling in lumbar (L) segments in intact andSCT<\/jats:styled-content>rats, with or without inclusion of 5\u2010week treadmill locomotor training. We show that at the second week afterSCT<\/jats:styled-content>the levels ofChrm2<\/jats:italic>transcript are reduced in the L3\u20136 segments, with [3<\/jats:sup>H]QNB<\/jats:styled-content>binding decreased selectively in the L5\u20136 segments, where ankle extensorMN<\/jats:styled-content>s are predominantly located. At 5\u00a0weeks afterSCT<\/jats:styled-content>, [3<\/jats:sup>H]QNB<\/jats:styled-content>binding differences between the L3\u20134 and L5\u20136 segments are maintained, accompanied by higher density of M2R immunolabeling in the plasma membrane and cytoplasm ofTA<\/jats:styled-content>than SolMN<\/jats:styled-content>s and by enriched synaptic versus extrasynaptic M2R pools (52%TA<\/jats:styled-content>vs. 25% SolMN<\/jats:styled-content>s). Training normalized M2R inTA MN<\/jats:styled-content>s, improved locomotion, and reduced frequency of clonic episodes. Our findings indicate higher sensitivity ofTA<\/jats:styled-content>than SolMN<\/jats:styled-content>s to cholinergic signaling afterSCT<\/jats:styled-content>, which might shorten flexor twitches duration and contribute to generation of clonic movements. Synaptic enrichment in M2R density may reflect a compensatory mechanism activated inTA<\/jats:styled-content>and SolMN<\/jats:styled-content>s to different extent in response to reduced strength of cholinergic signaling to eachMN<\/jats:styled-content>pool.<\/jats:p>Open Practices<\/jats:title><\/jats:p>Open Science: This manuscript was awarded with the Open Materials Badge.<\/jats:p>For more information see:https:\/\/cos.io\/our-services\/open-science-badges\/<\/jats:ext-link><\/jats:p><\/jats:sec><\/jats:sec>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text><\/jats:p><\/jats:sec>","DOI":"10.1111\/jnc.14567","type":"journal-article","created":{"date-parts":[[2018,8,13]],"date-time":"2018-08-13T16:36:58Z","timestamp":1534178218000},"page":"361-379","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Spinalization and locomotor training differentially affect muscarinic acetylcholine receptor type 2 abutting on \u03b1\u2010motoneurons innervating the ankle extensor and flexor muscles"],"prefix":"10.1111","volume":"147","author":[{"given":"Angelika","family":"Wi\u0119ckowska","sequence":"first","affiliation":[{"name":"Nencki Institute of Experimental Biology Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olga","family":"Gajewska\u2010Wo\u017aniak","sequence":"additional","affiliation":[{"name":"Nencki Institute of Experimental Biology Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"G\u0142owacka","sequence":"additional","affiliation":[{"name":"Nencki Institute of Experimental Biology Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benjun","family":"Ji","sequence":"additional","affiliation":[{"name":"Nencki Institute of Experimental Biology Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kamil","family":"Grycz","sequence":"additional","affiliation":[{"name":"Nencki Institute of Experimental Biology Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julita","family":"Czarkowska\u2010Bauch","sequence":"additional","affiliation":[{"name":"Nencki Institute of Experimental Biology Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8470-0941","authenticated-orcid":false,"given":"Ma\u0142gorzata","family":"Skup","sequence":"additional","affiliation":[{"name":"Nencki Institute of Experimental Biology Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,10,29]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2009.10.004"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.787ab.x"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1460-9568.2002.02088.x"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1235-06.2006"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1993.70.4.1300"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902290305"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.18-23-10207.1998"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1044-7431(03)00034-4"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep40155"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1359-13.2013"},{"key":"e_1_2_8_13_1","first-page":"372","article-title":"Alterations in neurotransmitter receptor binding in the spinal cord after transection","volume":"218","author":"Charlton G.","year":"1981","journal-title":"Brain Res."},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.104.082537"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresbull.2004.08.008"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2010.07350.x"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.1998.6831"},{"key":"e_1_2_8_18_1","first-page":"49","article-title":"Ultrastructure and distribution of neuronal and glial elements on the surface of the proximal part of a motoneuron dendrite, as analyzed by serial sections","volume":"332","author":"Conradi S.","year":"1969","journal-title":"Acta Physiol. 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