{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T09:41:07Z","timestamp":1790847667367,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,11,13]],"date-time":"2018-11-13T00:00:00Z","timestamp":1542067200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Michael J Fox Foundation"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>\u03b1\u2010Synuclein is the major component of Lewy bodies and a candidate biomarker for neurodegenerative diseases in which Lewy bodies are common, including Parkinson's disease and dementia with Lewy bodies. A large body of literature suggests that these disorders are characterized by reduced concentrations of \u03b1\u2010synuclein in cerebrospinal fluid (CSF), with overlapping concentrations compared to healthy controls and variability across studies. Several reasons can account for this variability, including technical ones, such as inter\u2010assay and inter\u2010laboratory variation (reproducibility). We compared four immunochemical methods for the quantification of \u03b1\u2010synuclein concentration in 50 unique CSF samples. All methods were designed to capture most of the existing \u03b1\u2010synuclein forms in CSF (\u2018total\u2019 \u03b1\u2010synuclein). Each of the four methods showed high analytical precision, excellent correlation between laboratories (<jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup> 0.83\u20130.99), and good correlation with each other (<jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup> 0.64\u20130.93), although the slopes of the regression lines were different between the four immunoassays. The use of common reference CSF samples decreased the differences in \u03b1\u2010synuclein concentration between detection methods and technologies. Pilot data on an immunoprecipitation mass spectrometry (IP\u2010MS) method is\u00a0also presented. Our results suggest that the four immunochemical methods and the IP\u2010MS method measure similar\u00a0forms of \u03b1\u2010synuclein and that a common reference material\u00a0would allow harmonization of results between immunoassays.<\/jats:p><\/jats:sec><jats:sec><jats:label\/><jats:p>\n<jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc14569-fig-0005-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text>\n<\/jats:p><\/jats:sec>","DOI":"10.1111\/jnc.14569","type":"journal-article","created":{"date-parts":[[2018,8,22]],"date-time":"2018-08-22T15:09:38Z","timestamp":1534950578000},"page":"126-138","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":52,"title":["Antibody\u2010based methods for the measurement of \u03b1\u2010synuclein concentration in human cerebrospinal fluid \u2013 method comparison and round robin study"],"prefix":"10.1111","volume":"149","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8437-3645","authenticated-orcid":false,"given":"Brit","family":"Mollenhauer","sequence":"first","affiliation":[{"name":"Paracelsus\u2010Elena\u2010Klinik Kassel Germany"},{"name":"Department of Neurology University Medical Center Goettingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frederick DuBois","family":"Bowman","sequence":"additional","affiliation":[{"name":"Department of Biostatistics Columbia University Mailman School of Public Health New York City New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Drake","sequence":"additional","affiliation":[{"name":"Department of Biostatistics Columbia University Mailman School of Public Health New York City New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jimmy","family":"Duong","sequence":"additional","affiliation":[{"name":"Department of Biostatistics Columbia University Mailman School of Public Health New York City New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaj","family":"Blennow","sequence":"additional","affiliation":[{"name":"Department of Psychiatry and Neurochemistry the Sahlgrenska Academy at the University of Gothenburg M\u00f6lndal Sweden"},{"name":"Clinical Neurochemistry Laboratory Sahlgrenska University Hospital M\u00f6lndal Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omar","family":"El\u2010Agnaf","sequence":"additional","affiliation":[{"name":"Neurological Disorders Research Center Qatar Biomedical Research Institute (QBRI), and College of Science and Engineering HBKU, Education City Qatar Foundation Doha Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leslie M.","family":"Shaw","sequence":"additional","affiliation":[{"name":"Department of Pathology &amp; Laboratory Medicine and Center for Neurodegenerative Disease Research Institute on Aging University of Pennsylvania Philadelphia PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Masucci","sequence":"additional","affiliation":[{"name":"BioLegend Dedham MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peggy","family":"Taylor","sequence":"additional","affiliation":[{"name":"BioLegend Dedham MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert M.","family":"Umek","sequence":"additional","affiliation":[{"name":"Meso Scale Discovery Gaithersburg MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jill M.","family":"Dunty","sequence":"additional","affiliation":[{"name":"Meso Scale Discovery Gaithersburg MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris L.","family":"Smith","sequence":"additional","affiliation":[{"name":"Meso Scale Discovery Gaithersburg MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erik","family":"Stoops","sequence":"additional","affiliation":[{"name":"ADx NeuroSciences Gent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hugo","family":"Vanderstichele","sequence":"additional","affiliation":[{"name":"ADx NeuroSciences Gent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrian W.","family":"Schmid","sequence":"additional","affiliation":[{"name":"Proteomics Core Facility Ecole Polytechnique F\u00e9d\u00e9rale de Lausanne (EPFL) Lausanne Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Moniatte","sequence":"additional","affiliation":[{"name":"Proteomics Core Facility Ecole Polytechnique F\u00e9d\u00e9rale de Lausanne (EPFL) Lausanne Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Zhang","sequence":"additional","affiliation":[{"name":"University of Washington Seattle WA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niels","family":"Kruse","sequence":"additional","affiliation":[{"name":"Institute of Neuropathology University Medical Center Goettingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hilal A.","family":"Lashuel","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular and Chemical Biology of Neurodegeneration Brain Mind Institute Institute of Physics of Biological Systems Ecole Polytechnique F\u00e9d\u00e9rale de Lausanne (EPFL) CH\u20101015 Lausanne Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlotte","family":"Teunissen","sequence":"additional","affiliation":[{"name":"VU University Medical Center Amsterdam the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tanja","family":"Schubert","sequence":"additional","affiliation":[{"name":"Bioclinica Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuldip D.","family":"Dave","sequence":"additional","affiliation":[{"name":"Michael J. 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