{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T22:30:28Z","timestamp":1775255428108,"version":"3.50.1"},"reference-count":65,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,12,5]],"date-time":"2018-12-05T00:00:00Z","timestamp":1543968000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI14\/00876"],"award-info":[{"award-number":["PI14\/00876"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI051871, CIBERehd"],"award-info":[{"award-number":["PI051871, CIBERehd"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2019,2]]},"abstract":"Abstract<\/jats:title>\n \n \n \n High catecolamine plasma levels because of sympathetic nervous system over\u2010activity contribute to cirrhosis progression. The aim of this study was to investigate whether chromaffin cells of the adrenal gland might potentiate the deleterious effect exerted by this over\u2010activity. Electrophysiological patch\u2010clamp and amperometric experiments with carbon\u2010fibre electrodes were conducted in single chromaffin cells of control and\n CC<\/jats:styled-content>\n l\n 4<\/jats:sub>\n \u2010induced cirrhotic rats. The spontaneous action potential firing frequency was increased in chromaffin cells of cirrhotic rats with respect to control rats. The exocytosis evoked by that firing was also increased. However, exocytosis elicited by\n AC<\/jats:styled-content>\n h did not vary between control and cirrhotic rats. Exocytosis triggered by depolarizing pulses was also unchanged. Amperometric recordings confirmed the lack of increased catecholamine charge released in cirrhosis after\n AC<\/jats:styled-content>\n h or depolarization stimuli. However, the amperometric spikes exhibited faster kinetics of release. The overall Ca\n 2+<\/jats:sup>\n entry through voltage\u2010dependent Ca\n 2+<\/jats:sup>\n channels (\n VDCC<\/jats:styled-content>\n ), or in particular through Cav1 channels, did not vary between chromaffin cells of control and cirrhotic rats. The inhibition of\n VDCC<\/jats:styled-content>\n by methionine\u2010enkephaline or\n ATP<\/jats:styled-content>\n was not either altered, but it was increased by adrenaline in cells of cirrhotic rats. When a cocktail composed by the three neurotransmitters was tested in order to approach a situation closer to the physiological condition, the inhibition of\n VDCC<\/jats:styled-content>\n was similar between both types of cells. In summary, chromaffin cells of the adrenal gland might contribute to exacerbate the sympathetic nervous system over\u2010activity in cirrhosis because of an increased exocytosis elicited by an enhanced spontaneous electrical activity.\n <\/jats:p>\n <\/jats:sec>\n \n \n \n \n \n image<\/jats:alt-text>\n <\/jats:graphic>\n <\/jats:boxed-text>\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/jnc.14618","type":"journal-article","created":{"date-parts":[[2018,10,22]],"date-time":"2018-10-22T18:46:01Z","timestamp":1540233961000},"page":"359-372","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["The firing frequency of spontaneous action potentials and their corresponding evoked exocytosis are increased in chromaffin cells of\n CC<\/scp>\n l\n 4<\/sub>\n \u2010induced cirrhotic rats with respect to control rats"],"prefix":"10.1111","volume":"148","author":[{"given":"Sara","family":"Sanz\u2010L\u00e1zaro","sequence":"first","affiliation":[{"name":"Departamento de Farmacolog\u00eda y Terap\u00e9utica Facultad de Medicina Universidad Aut\u00f3noma de Madrid Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanda","family":"Jim\u00e9nez\u2010Pompa","sequence":"additional","affiliation":[{"name":"Departamento de Farmacolog\u00eda y Terap\u00e9utica Facultad de Medicina Universidad Aut\u00f3noma de Madrid Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beatriz","family":"Carmona\u2010Hidalgo","sequence":"additional","affiliation":[{"name":"Departamento de Farmacolog\u00eda y Terap\u00e9utica Facultad de Medicina Universidad Aut\u00f3noma de Madrid Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda","family":"Ubeda","sequence":"additional","affiliation":[{"name":"Laboratorio de Enfermedades del 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