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Dysfunction in dopaminergic neurotransmission within the striatum<\/jats:italic> leads to movement impairments, gaiting defects, and hypo\u2010locomotion. Due to their high polarity and extreme axonal arborization, neurons depend on molecular motor proteins and microtubule\u2010based transport for their normal function. Transport defects have been associated with neurodegeneration since axonopathies, axonal clogging, microtubule destabilization, and lower motor proteins levels were described in the brain of patients with Parkinson's Disease and other neurodegenerative disorders. However, the contribution of specific motor proteins to the regulation of the nigrostriatal network remains unclear. Here, we generated different conditional knockout mice for the kinesin heavy chain 5B subunit (Kif5b) of Kinesin\u20101 to unravel its contribution to locomotion. Interestingly, mice with neuronal Kif5b<\/jats:italic> deletion showed hypo\u2010locomotion, movement initiation deficits, and coordination impairments. High pressure liquid chromatography determined that dopamine (DA<\/jats:styled-content>) metabolism is impaired in neuronal Kif5b<\/jats:italic>\u2010KO<\/jats:styled-content>, while no dopaminergic cell loss was observed. However, the deletion of Kif5b<\/jats:italic> only in dopaminergic neurons is not sufficient to induce locomotor defects. Noteworthy, pharmacological stimulation of DA<\/jats:styled-content> release together with agonist or antagonist of DA<\/jats:styled-content> receptors revealed selective D2\u2010dependent movement initiation defects in neuronal Kif5b<\/jats:italic>\u2010KO<\/jats:styled-content>. Finally, subcellular fractionation from striatum<\/jats:italic> showed that Kif5b<\/jats:italic> deletion reduced the amount of dopamine D2 receptor in synaptic plasma membranes. Together, these results revealed an important role for Kif5b in the modulation of the striatal network that is relevant to the overall locomotor response.<\/jats:p><\/jats:sec>Open science badges<\/jats:title><\/jats:p>This article has received a badge for *Open Materials* because it provided all relevant information to reproduce the study in the manuscript. The complete Open Science Disclosure form for this article can be found at the end of the article. More information about the Open Practices badges can be found at https:\/\/cos.io\/our-services\/open-science-badges\/<\/jats:ext-link>.<\/jats:p><\/jats:sec>\nimage<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text>\n<\/jats:p><\/jats:sec>","DOI":"10.1111\/jnc.14665","type":"journal-article","created":{"date-parts":[[2019,1,21]],"date-time":"2019-01-21T14:11:21Z","timestamp":1548079881000},"page":"362-380","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Neuronal KIF<\/scp>5b<\/i> deletion induces striatum<\/i>\u2010dependent locomotor impairments and defects in membrane presentation of dopamine D2 receptors"],"prefix":"10.1111","volume":"149","author":[{"given":"Lucas E.","family":"Cromberg","sequence":"first","affiliation":[{"name":"Instituto de Biolog\u00eda Celular y Neurociencias IBCN (CONICET\u2010UBA) Facultad de Medicina Universidad de Buenos Aires Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trinidad M. M.","family":"Saez","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda Celular y Neurociencias IBCN (CONICET\u2010UBA) Facultad de Medicina Universidad de Buenos Aires Buenos Aires Argentina"},{"name":"Instituto de Biolog\u00eda y Medicina Experimental IBYME (CONICET) Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda G.","family":"Otero","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda Celular y Neurociencias IBCN (CONICET\u2010UBA) Facultad de Medicina Universidad de Buenos Aires Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eugenia","family":"Tomasella","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda y Medicina Experimental IBYME (CONICET) Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mat\u00edas","family":"Alloatti","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda Celular y Neurociencias IBCN (CONICET\u2010UBA) Facultad de Medicina Universidad de Buenos Aires Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Damianich","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Farmacol\u00f3gicas ININFA, (CONICET\u2010UBA) Buenos Aires Argentina"},{"name":"Instituto de Investigaciones en Ingenier\u00eda Gen\u00e9tica y Biolog\u00eda Molecular INGEBI (CONICET) Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victorio","family":"Pozo Devoto","sequence":"additional","affiliation":[{"name":"Center for Translational Medicine (CTM) International Clinical Research Center St. Anne's University Hospital (ICRC\u2010FNUSA) Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Ferrario","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Farmacol\u00f3gicas ININFA, (CONICET\u2010UBA) Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego","family":"Gelman","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda y Medicina Experimental IBYME (CONICET) Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo","family":"Rubinstein","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones en Ingenier\u00eda Gen\u00e9tica y Biolog\u00eda Molecular INGEBI (CONICET) Buenos Aires Argentina"},{"name":"Facultad de Ciencias Exactas y Naturales Universidad de Buenos Aires Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7984-1149","authenticated-orcid":false,"given":"Tom\u00e1s L.","family":"Falzone","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda Celular y Neurociencias IBCN (CONICET\u2010UBA) Facultad de Medicina Universidad de Buenos Aires 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