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We previously showed that DHA is a substrate of fatty acid transport protein 1 (FATP1\/SLC27A1), and FATP1 is localized at the abluminal membrane of brain capillary endothelial cells. We hypothesized that amyloid \u03b2 (A\u03b2) decreases FATP1\u2010mediated cellular efflux (i.e. supply to the brain) of DHA at the blood\u2013brain barrier (BBB). Here, we tested this hypothesis using a human cerebral microvascular endothelial cell line, human cerebral microvessel endothelial cells (hCMEC\/D3), as a BBB model. The efflux of DHA\u2010d5 by hCMEC\/D3 cells increased time\u2010dependently up to 3\u00a0min. Knock\u2010down of FATP1 with specific siRNA indicated that FATP1\u2010mediated efflux accounts for 47.0% of this DHA\u2010d5 efflux. In hCMEC\/D3 cells treated with A\u03b2<jats:sub>25\u201335<\/jats:sub> (10\u00a0\u03bcM\/24\u00a0h), which we employed as an in vitro model of the BBB in AD, FATP1 protein expression in the plasma membrane was decreased by 96.0%, which was greater than the decrease in the whole\u2010cell lysate, and the DHA\u2010d5 efflux was decreased by 68.3%. Of this 68.3% decrease, 45.1% (47.0\u00a0\u00d7\u00a00.96) is accounted for by the decrease in FATP1\u2010mediated efflux and the remaining 23.2% is presumably mediated by other mechanism(s). Thus, we have established for the first time that FATP1 is a major contributor to DHA efflux from human brain capillary endothelial cells, and its efflux activity at the abluminal membrane of the cells is blocked by A\u03b2. This may explain the decreased DHA level in the brain of AD patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Open Science Badges<\/jats:title><jats:p><jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/jnc14722-gra-0001.png\" xlink:title=\"image\"\/><\/jats:p><jats:p>This article has received a badge for *Open Materials* because it provided all relevant information to reproduce the study in the manuscript. The complete Open Science Disclosure form for this article can be found at the end of the article. More information about the Open Practices badges can be found at <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"https:\/\/cos.io\/our-services\/open-science-badges\/\">https:\/\/cos.io\/our-services\/open-science-badges\/<\/jats:ext-link>.<\/jats:p><\/jats:sec><jats:sec><jats:label\/><jats:p>\n<jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc14722-fig-0006-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text>\n<\/jats:p><\/jats:sec>","DOI":"10.1111\/jnc.14722","type":"journal-article","created":{"date-parts":[[2019,5,15]],"date-time":"2019-05-15T16:38:45Z","timestamp":1557938325000},"page":"385-401","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Amyloid beta<sub>25\u201035<\/sub> impairs docosahexaenoic acid efflux by down\u2010regulating fatty acid transport protein 1 (FATP1\/SLC27A1) protein expression in human brain capillary endothelial cells"],"prefix":"10.1111","volume":"150","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0402-8137","authenticated-orcid":false,"given":"Yusuke","family":"Ochiai","sequence":"first","affiliation":[{"name":"Graduate School of Pharmaceutical Sciences Tohoku University Sendai Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuo","family":"Uchida","sequence":"additional","affiliation":[{"name":"Graduate School of Pharmaceutical Sciences Tohoku University Sendai Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masanori","family":"Tachikawa","sequence":"additional","affiliation":[{"name":"Graduate School of Pharmaceutical Sciences Tohoku University Sendai 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