{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T04:31:56Z","timestamp":1776400316339,"version":"3.51.2"},"reference-count":43,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,11,21]],"date-time":"2019-11-21T00:00:00Z","timestamp":1574294400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81571615"],"award-info":[{"award-number":["81571615"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Major histocompatibility Complex class I (MHC I) molecules are ubiquitously expressed, being found in most nucleated cells, where they are central mediators of both the adaptive and innate immune responses. Recent studies have shown that MHC I are also expressed in the developing brain where they participate in synapse elimination and plasticity. Up\u2010regulation of MHC I within the developing brain has been reported, however, the mechanism(s) regulating this developmental up\u2010regulation of neuronal MHC I remains unknown. Here, we show NLR family CARD domain containing 5 (NLRC5), a newly identified member of the NLR family, is widely expressed in hippocampal neurons, and the expression pattern of NLRC5 coincides with increased MHC I mRNA in the developing hippocampus. Using a luciferase assay in Neuro\u20102a cells we demonstrate that NLRC5 can induce the activation of MHC I and this induction requires the W\/S\u2010X\u2010Y motif. Further studies show that transcription factors regulatory factor X (RFX) and CREB1, which bind to X1 and X2 box, are crucial for NLRC5\u2010mediated induction. Moreover immunoprecipitation experiments reveal that NLRC5 interacts with RFX subunits RFX5 and RFXANK. Knockout of <jats:italic>Nlrc5<\/jats:italic> dramatically impairs basal expression of MHC I in mouse hippocampus. Taken together, our findings identify NLRC5 as a key regulator of MHC I up\u2010regulation in the developing hippocampus and suggest an important role for NLRC5 in neurons.<\/jats:p><\/jats:sec><jats:sec><jats:label\/><jats:p>\n<jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc14876-fig-0010-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text>\n<\/jats:p><jats:p>Cover Image for this issue: doi: <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1111\/jnc.14729\">10.1111\/jnc.14729<\/jats:ext-link>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jnc.14876","type":"journal-article","created":{"date-parts":[[2019,9,24]],"date-time":"2019-09-24T10:22:10Z","timestamp":1569320530000},"page":"182-194","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Neuronal NLRC5 regulates MHC class I expression in Neuro\u20102a cells and also during hippocampal development"],"prefix":"10.1111","volume":"152","author":[{"given":"Ping","family":"Li","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology Key Laboratory of Developmental Genes and Human Disease Ministry of Education Medical School Southeast University Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuqing","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Key Laboratory of Developmental Genes and Human Disease Ministry of Education Medical School Southeast University Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pengfei","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Key Laboratory of Developmental Genes and Human Disease Ministry of Education Medical School Southeast University Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Key Laboratory of Developmental Genes and Human Disease Ministry of Education Medical School Southeast University Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Key Laboratory of Developmental Genes and Human Disease Ministry of Education Medical School Southeast University Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fengqin","family":"Miao","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Key Laboratory of Developmental Genes and Human Disease Ministry of Education Medical School Southeast University Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aifeng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathology Medical School Southeast University Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7446-6397","authenticated-orcid":false,"given":"Jianqiong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Key Laboratory of Developmental Genes and Human Disease Ministry of Education Medical School Southeast University Nanjing China"},{"name":"Jiangsu key laboratory of Molecular and Functional Imaging Zhongda Hospital Medical School Southeast University Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,11,21]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2018.02.001"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0903900"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneuroim.2012.03.008"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1200064"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80681-0"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80562-0"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2009.10.015"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/biof.1285"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0914064107"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2764"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.9.5175"},{"key":"e_1_2_7_13_1","first-page":"36\u2010","article-title":"Dnmt1 regulates expression of MHC class I in post\u2010mitotic neurons. 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