{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:51:44Z","timestamp":1772160704217,"version":"3.50.1"},"reference-count":55,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,11,28]],"date-time":"2019-11-28T00:00:00Z","timestamp":1574899200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002923","name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","doi-asserted-by":"publisher","award":["PIP 11220150100858CO"],"award-info":[{"award-number":["PIP 11220150100858CO"]}],"id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>Synaptic strength depends on the number of cell\u2010surface neurotransmitter receptors in dynamic equilibrium with intracellular pools. Dysregulation of this homeostatic balance occurs, for example in myasthenia gravis, an autoimmune disease characterized by a decrease in the number of postsynaptic nicotinic acetylcholine receptors (nAChRs). Monoclonal antibody mAb35 mimics this effect. Here we use STORM nanoscopy to characterize the individual and ensemble dynamics of monoclonal antibody\u2010crosslinked receptors in the clonal cell line CHO\u2010K1\/A5, which robustly expresses adult muscle\u2010type nAChRs. Antibody labeling of live cells results in 80% receptor immobilization. The remaining mobile fraction exhibits a heterogeneous combination of Brownian and anomalous diffusion. Single\u2010molecule trajectories exhibit a two\u2010state switching behavior between free Brownian walks and anticorrelated walks within confinement areas. The latter act as permeable fences (~34\u00a0nm radius, ~400\u00a0ms lifetime). Dynamic clustering, trapping, and immobilization also occur in larger nanocluster zones (120\u2013180\u00a0nm radius) with longer lifetimes (11\u00a0\u00b1\u00a01\u00a0s), in a strongly cholesterol\u2010sensitive manner. Cholesterol depletion increases the size of the clustering phenomenon; cholesterol enrichment has the opposite effect. The disclosed high proportion of monoclonal antibody\u2010crosslinked immobile receptors, together with their anomalous, cholesterol\u2010sensitive diffusion and clustering, provides new insights into the antibody\u2010enhanced antigenic modulation that leads to physiopathological internalization and degradation of receptors in myasthenia.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      <jats:boxed-text content-type=\"graphic\" position=\"anchor\">\n                        <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc14905-fig-0005-m.png\">\n                          <jats:alt-text>image<\/jats:alt-text>\n                        <\/jats:graphic>\n                      <\/jats:boxed-text>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jnc.14905","type":"journal-article","created":{"date-parts":[[2019,11,28]],"date-time":"2019-11-28T06:20:15Z","timestamp":1574922015000},"page":"663-674","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Antibody\u2010induced crosslinking and cholesterol\u2010sensitive, anomalous diffusion of nicotinic acetylcholine receptors"],"prefix":"10.1111","volume":"152","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5693-5302","authenticated-orcid":false,"given":"Alejo","family":"Mosqueira","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Neurobiology Biomedical Research institute (BIOMED) UCA\u2013CONICET Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3932-3259","authenticated-orcid":false,"given":"Pablo A.","family":"Camino","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Neurobiology Biomedical Research institute (BIOMED) UCA\u2013CONICET Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4745-681X","authenticated-orcid":false,"given":"Francisco J.","family":"Barrantes","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Neurobiology Biomedical Research institute (BIOMED) UCA\u2013CONICET Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,11,28]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.286.5439.503"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(02)00739-0"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0100346"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-25454-0"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0022678"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.92.1.60"},{"key":"e_1_2_8_8_1","first-page":"1","volume-title":"Springer Protocols","author":"Barrantes F. 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