{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T09:22:37Z","timestamp":1790846557168,"version":"4.1.0"},"reference-count":49,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,1,8]],"date-time":"2020-01-08T00:00:00Z","timestamp":1578441600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      The olfactory mucosa, where the first step of odor detection occurs, is a privileged pathway for environmental toxicants and pathogens toward the central nervous system. Indeed, some pathogens can infect olfactory sensory neurons including their axons projecting to the olfactory bulb allowing them to bypass the blood\u2013brain barrier and reach the central nervous system (CNS) through the so\u2010called olfactory pathway. The respiratory syncytial virus (RSV) is a major respiratory tract pathogen but there is growing evidence that RSV may lead to CNS impairments. However, the mechanisms involved in RSV entering into the CNS have been poorly described. In this study, we wanted to explore the capacity of RSV to reach the CNS\n                      <jats:italic>via<\/jats:italic>\n                      the olfactory pathway and to better characterize RSV cellular tropism in the nasal cavity. We first explored the distribution of RSV infectious sites in the nasal cavity by in vivo bioluminescence imaging and a tissue clearing protocol combined with deep\u2010tissue imaging and 3D image analyses. This whole tissue characterization was confirmed with immunohistochemistry and molecular biology approaches. Together, our results provide a novel 3D atlas of mouse nasal cavity anatomy and show that RSV can infect olfactory sensory neurons giving access to the central nervous system by entering the olfactory bulb.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      <jats:boxed-text content-type=\"graphic\" position=\"anchor\">\n                        <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc14936-fig-0009-m.png\">\n                          <jats:alt-text>image<\/jats:alt-text>\n                        <\/jats:graphic>\n                      <\/jats:boxed-text>\n                    <\/jats:p>\n                    <jats:p>\n                      Cover Image for this issue: doi:\n                      <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1111\/jnc.14765\">10.1111\/jnc.14765<\/jats:ext-link>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jnc.14936","type":"journal-article","created":{"date-parts":[[2019,12,7]],"date-time":"2019-12-07T09:16:55Z","timestamp":1575710215000},"page":"137-153","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":52,"title":["Respiratory syncytial virus tropism for olfactory sensory neurons in mice"],"prefix":"10.1111","volume":"155","author":[{"given":"Bertrand","family":"Bryche","sequence":"first","affiliation":[{"name":"NBO INRA Univ Paris\u2010Saclay Jouy\u2010en\u2010Josas France"},{"name":"Universit\u00e9 de Versailles Saint\u2010Quentin en Yvelines Versailles France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maxence","family":"Fr\u00e9taud","sequence":"additional","affiliation":[{"name":"VIM INRA Univ Paris Saclay Jouy\u2010en\u2010Josas France"},{"name":"INRA EMERG'IN\u2010 Plateforme d'Infectiologie Exp\u00e9rimentale IERP\u2010 Domaine de Vilvert Jouy\u2010en\u2010Josas France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Audrey","family":"Saint\u2010Albin Deliot","sequence":"additional","affiliation":[{"name":"NBO INRA Univ Paris\u2010Saclay Jouy\u2010en\u2010Josas France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Galloux","sequence":"additional","affiliation":[{"name":"VIM INRA Univ Paris Saclay Jouy\u2010en\u2010Josas France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Sedano","sequence":"additional","affiliation":[{"name":"VIM INRA Univ Paris Saclay Jouy\u2010en\u2010Josas France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christelle","family":"Langevin","sequence":"additional","affiliation":[{"name":"VIM INRA Univ Paris Saclay Jouy\u2010en\u2010Josas France"},{"name":"INRA EMERG'IN\u2010 Plateforme d'Infectiologie Exp\u00e9rimentale IERP\u2010 Domaine de Vilvert Jouy\u2010en\u2010Josas France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Delphyne","family":"Descamps","sequence":"additional","affiliation":[{"name":"VIM INRA Univ Paris Saclay Jouy\u2010en\u2010Josas France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie\u2010Anne","family":"Rameix\u2010Welti","sequence":"additional","affiliation":[{"name":"UMR INSERM U1173 I2 UFR des Sciences de la Sant\u00e9 Simone Veil\u2014UVSQ Montigny\u2010Le\u2010Bretonneux France"},{"name":"Assistance Publique\u2010H\u00f4pitaux de Paris Laboratoire de Microbiologie H\u00f4pital Ambroise Par\u00e9 Boulogne\u2010Billancourt France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Fran\u00e7ois","family":"El\u00e9ou\u00ebt","sequence":"additional","affiliation":[{"name":"VIM INRA Univ Paris Saclay Jouy\u2010en\u2010Josas France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2012-0064","authenticated-orcid":false,"given":"Ronan","family":"Le Goffic","sequence":"additional","affiliation":[{"name":"VIM INRA Univ Paris Saclay Jouy\u2010en\u2010Josas France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2769-647X","authenticated-orcid":false,"given":"Nicolas","family":"Meunier","sequence":"additional","affiliation":[{"name":"NBO INRA Univ Paris\u2010Saclay Jouy\u2010en\u2010Josas France"},{"name":"Universit\u00e9 de Versailles Saint\u2010Quentin en Yvelines Versailles France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,1,8]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"29","article-title":"The lateral nasal gland of dog: Its structure and secretory content","volume":"132","author":"Adams D. R.","year":"1981","journal-title":"Journal of Anatomy"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-016-2880-1"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/ACI.0b013e3280114eef"},{"issue":"63","key":"e_1_2_8_5_1","first-page":"1","article-title":"Anatomy, histochemistry, and immunohistochemistry of the olfactory subsystems in mice","volume":"8","author":"Barrios A. W.","year":"2014","journal-title":"Frontiers in Neuroanatomy"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11307-017-1099-1"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0213545"},{"issue":"386","key":"e_1_2_8_8_1","first-page":"1","article-title":"Neurologic alterations due to respiratory virus infections","volume":"12","author":"Bohmwald K.","year":"2018","journal-title":"Frontiers in Cellular Neuroscience"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1091500103"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2019.02.012"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1006\/geno.1994.1200"},{"key":"e_1_2_8_12_1","first-page":"e3960","article-title":"Examining the role of nasopharyngeal\u2010associated Lymphoreticular Tissue (NALT) in mouse responses to vaccines","volume":"66","author":"Cisney E. D.","year":"2012","journal-title":"Journal of Visualized Experiments"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0022215100049690"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00118-13"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/emmm.201202124"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/acschemneuro.6b00043"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(06)69409-9"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1217508110"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-10-305"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41583-018-0070-8"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.68.6.813"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/ejn.13600"},{"issue":"24687","key":"e_1_2_8_23_1","first-page":"1","article-title":"Olfactory epithelium changes in germfree mice","volume":"6","author":"Fran\u00e7ois A.","year":"2016","journal-title":"Scientific Reports"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2008.06240.x"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics10030116"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aah6518"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.22735"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10156-012-0418-3"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.5.14.1752363"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1369\/jhc.3A6243.2004"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-018-3847-1"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.11613\/BM.2013.014"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri1439"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.11.5416-5420.2001"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.24250"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1177\/019459989210600221"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5320(71)80093-X"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2012.00431.x"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2005.01.023"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10156-011-0259-5"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/cmi.2017.150"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.7.2383"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.23736\/S0026-4946.18.05312-4"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms6104"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.05.007"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00055.2016"},{"key":"e_1_2_8_47_1","doi-asserted-by":"crossref","first-page":"13971","DOI":"10.1016\/S0021-9258(18)68169-4","article-title":"Molecular mechanisms of olfaction","volume":"263","author":"Snyder S. H.","year":"1988","journal-title":"Journal of Biological Chemistry"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.2500\/ajra.2010.24.3435"},{"issue":"13","key":"e_1_2_8_49_1","first-page":"1","article-title":"Respiratory syncytial virus prolifically infects N2a neuronal cells, leading to TLR4 and nucleolin protein modulations and RSV F protein co\u2010localization with TLR4 and nucleolin","volume":"25","author":"Yuan X.","year":"2018","journal-title":"Journal of Biomedical Science"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2009.06.002"}],"container-title":["Journal of Neurochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjnc.14936","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jnc.14936","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jnc.14936","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jnc.14936","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,4]],"date-time":"2023-09-04T12:48:28Z","timestamp":1693831708000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jnc.14936"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,8]]},"references-count":49,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2020,10]]}},"alternative-id":["10.1111\/jnc.14936"],"URL":"https:\/\/doi.org\/10.1111\/jnc.14936","archive":["Portico"],"relation":{},"ISSN":["0022-3042","1471-4159"],"issn-type":[{"value":"0022-3042","type":"print"},{"value":"1471-4159","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1,8]]},"assertion":[{"value":"2019-10-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-12-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-01-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}