{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T05:12:36Z","timestamp":1790745156123,"version":"4.1.0"},"reference-count":73,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,1,21]],"date-time":"2020-01-21T00:00:00Z","timestamp":1579564800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000026","name":"National Institute on Drug Abuse","doi-asserted-by":"publisher","award":["R01DA829212"],"award-info":[{"award-number":["R01DA829212"]}],"id":[{"id":"10.13039\/100000026","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>It has been shown that prenatal nicotine and tobacco smoke exposure can cause different neurobehavioral disorders in the offspring. We hypothesize that prenatal exposure to nicotine\u2010containing electronic cigarette (e\u2010Cig) vapor can predispose newborn to enhanced sensitivity to hypoxic\u2013ischemic (HI) brain injury and impaired motor and cognitive functions. In this study, pregnant CD1 mice were exposed to e\u2010Cig vapor (2.4% nicotine). Primary cortical neurons isolated from e\u2010Cig exposed fetus were exposed to oxygen\u2013glucose deprivation followed by reoxygenation (OGD\/R) to mimic HI brain injury. Cell viability and glucose utilization were analyzed in these neurons. HI brain injury was induced in 8\u20139\u2010day\u2010old pups. Short\u2010term brain injury was evaluated by triphenyltetrazolium chloride staining. Long\u2010term motor and cognitive functions were evaluated by open field, novel object recognition, Morris water maze, and foot fault tests. Western blotting and immunofluorescence were done to characterize glucose transporters in offspring brain. We found that e\u2010Cig exposed neurons demonstrated decreased cell viability and glucose utilization in OGD\/R. Prenatally e\u2010Cig exposed pups also had increased brain injury and edema 24\u00a0hr after HI brain injury. Further, in utero e\u2010Cig exposed offspring with HI brain injury displayed impaired memory, learning, and motor coordination at adolescence. Additionally, the expression of glucose transporters decreased in e\u2010Cig exposed offspring brain after HI brain injury. These results indicate that reduced glucose utilization can contribute to prenatal e\u2010Cig exposure induced worsened HI brain injury in offspring. This study is instrumental in elucidating the possible deleterious effects of e\u2010Cig use in the general population.<\/jats:p><\/jats:sec><jats:sec><jats:label\/><jats:p>\n<jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc14947-fig-0011-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text>\n<\/jats:p><\/jats:sec>","DOI":"10.1111\/jnc.14947","type":"journal-article","created":{"date-parts":[[2019,12,28]],"date-time":"2019-12-28T20:14:45Z","timestamp":1577564085000},"page":"63-79","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Prenatal electronic cigarette exposure decreases brain glucose utilization and worsens outcome in offspring hypoxic\u2013ischemic brain 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