{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T18:53:45Z","timestamp":1789757625397,"version":"4.0.1"},"reference-count":46,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,3,24]],"date-time":"2020-03-24T00:00:00Z","timestamp":1585008000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Neuropathic pain is an unfavorable pathological pain, often persistent over time, thus leading to significant impairment of quality of life and public health burden. Notably, microRNAs have been implicated in the pathophysiological process of neuropathic pain. The potential mechanism by which miR\u201034c\u20105p functions in neuropathic pain remains unclear. This study aimed to test the hypothesis that miR\u201034c\u20105p can modulate neuropathic pain in rat models with chronic constriction injury (CCI) of sciatic nerve, <jats:italic>via<\/jats:italic> interaction with the SIRT1\/STAT3 signaling pathway SIRT1 was validated as a target gene of miR\u201034c\u20105p and could be negatively regulated by miR\u201034c\u20105p. We induced miR\u201034c\u20105p overexpression\/inhibition, SIRT1 knockdown, and STAT3 knockdown in the model rats to assess pain behavior patterns. Meanwhile, dorsal root ganglion (DRG) was transduced with overexpression or knockdown of miR\u201034c\u20105p or lipopolysaccharide to induce the production of inflammatory factors. It was observed that miR\u201034c\u20105p was up\u2010regulated, and SIRT1 was under\u2010expressed in the DRG neurons of dorsal spinal cords of the CCI rats. Furthermore, the ectopic expression of miR\u201034c\u20105p and knockdown of SIRT1 in CCI rats resulted in increased hyperalgesia and inflammation, corresponding to reduced paw withdrawal threshold and paw withdrawal latency, and elevated levels of IL\u20106, IL\u20101\u03b2, and TNF\u2010\u03b1. More importantly, miR\u201034c\u20105p inhibition reduced the hyperalgesia and inflammation by blocking the STAT3 signaling pathway through up\u2010regulation of SIRT1. Conjointly, our results indicated that the down\u2010regulation of miR\u201034c\u20105p could potentially provide sustained relief in neuropathic pain by promoting SIRT1 expression through STAT3 signaling pathway inactivation.<\/jats:p><\/jats:sec><jats:sec><jats:label\/><jats:p>\n<jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc14998-fig-0010-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text>\n<\/jats:p><\/jats:sec>","DOI":"10.1111\/jnc.14998","type":"journal-article","created":{"date-parts":[[2020,3,3]],"date-time":"2020-03-03T19:22:17Z","timestamp":1583263337000},"page":"301-315","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Down\u2010regulation of microRNA\u201034c\u20105p alleviates neuropathic pain <i>via<\/i> the SIRT1\/STAT3 signaling pathway in rat models of chronic constriction injury of sciatic nerve"],"prefix":"10.1111","volume":"154","author":[{"given":"Yanshuai","family":"Mo","sequence":"first","affiliation":[{"name":"Department of Anesthesiology Linyi People\u2019s Hospital Linyi P.R. 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