{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T20:10:46Z","timestamp":1785528646341,"version":"3.56.0"},"reference-count":78,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,10,14]],"date-time":"2020-10-14T00:00:00Z","timestamp":1602633600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Perivascular pericytes are key regulators of the blood\u2013brain barrier, vascular development, and cerebral blood flow. Deciphering pericyte roles in health and disease requires cellular tracking; yet, pericyte identification remains challenging. A previous study reported that the far\u2010red fluorophore TO\u2010PRO\u20103 (642\/661), usually employed as a nuclear dye in fixed tissue, was selectively captured by live pericytes from the subventricular zone. Herein, we validated TO\u2010PRO\u20103 as a specific pericyte tracer in the nervous system (NS). Living pericytes from ex vivo murine hippocampus, cortex, spinal cord, and retina robustly incorporated TO\u2010PRO\u20103. Classical pericyte immunomarkers such as chondroitin sulphate proteoglycan neuron\u2010glial antigen 2 (NG2) and platelet\u2010derived growth factor receptor beta antigen (PDGFr\u03b2) and the new pericyte dye NeuroTrace 500\/525 confirmed cellular specificity of dye uptake. The TO\u2010PRO\u20103 signal enabled quantification of pericytes density and morphometry; likewise, TO\u2010PRO\u20103 labeling allowed visualization of pericytes associated with other components of the neurovascular unit. A subset of TO\u2010PRO\u20103 stained cells expressed the contractile protein \u03b1\u2013SMA, indicative of their ability to control the capillary diameter. Uptake of TO\u2010PRO\u20103 was independent of connexin\/pannexin channels but was highly sensitive to temperature and showed saturation, suggesting that a yet unidentified protein\u2010mediated active transport sustained dye incorporation. We conclude that TO\u2010PRO\u20103 labeling provides a reliable and simple tool for the bioimaging of pericytes in the murine NS microvasculature.<\/jats:p><\/jats:sec><jats:sec><jats:label\/><jats:p>\n<jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc15193-fig-0009-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text>\n<\/jats:p><\/jats:sec>","DOI":"10.1111\/jnc.15193","type":"journal-article","created":{"date-parts":[[2020,9,25]],"date-time":"2020-09-25T05:41:37Z","timestamp":1601012497000},"page":"1377-1391","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["A nuclear fluorescent dye identifies pericytes at the neurovascular unit"],"prefix":"10.1111","volume":"157","author":[{"given":"Sandra P.","family":"Mai\u2010Morente","sequence":"first","affiliation":[{"name":"Departamento de Fisiolog\u00eda Facultad de Medicina Universidad de la Rep\u00fablica Montevideo Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginia M.","family":"Marset","sequence":"additional","affiliation":[{"name":"Departamento de Fisiolog\u00eda Facultad de Medicina Universidad de la Rep\u00fablica Montevideo Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabiana","family":"Blanco","sequence":"additional","affiliation":[{"name":"Departamento de Biof\u00edsica Facultad de Medicina Universidad de la Rep\u00fablica Montevideo Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eugenia E.","family":"Isasi","sequence":"additional","affiliation":[{"name":"Departamento de Histolog\u00eda y Embriolog\u00eda Facultad de Medicina Universidad de la Rep\u00fablica Montevideo Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0703-306X","authenticated-orcid":false,"given":"Ver\u00f3nica","family":"Abudara","sequence":"additional","affiliation":[{"name":"Departamento de Fisiolog\u00eda Facultad de Medicina Universidad de la Rep\u00fablica Montevideo Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,10,14]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2009.07.030"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.22785"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.34861"},{"key":"e_1_2_9_5_1","volume-title":"Molecular Biology of the Cell","author":"Alberts B.","year":"2002"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature09522"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X15610340"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1011965307612"},{"key":"e_1_2_9_9_1","volume-title":"Berne & levy physiology","author":"Berne R. 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