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Critical roles of the \u03b1\u2010subunits of Gi\/o family heterotrimeric G\u2010proteins, G\u03b1i2, and G\u03b1o1, have so far been reported in brain development and neurodevelopmental disorders. In this study, we tried to clarify the role of G\u03b1i1, \u03b1\u2010subunit of another Gi\/o family member Gi1, during corticogenesis, based on the recent identification of its gene abnormalities in neurodevelopmental disorders. In western blot analyses, G\u03b1i1 was found to be expressed in mouse brain in a developmental stage\u2010dependent manner. Morphological analyses revealed that G\u03b1i1 was broadly distributed in cerebral cortex with relatively high expression in the ventricular zone (VZ) at embryonic day (E) 14. Meanwhile, G\u03b1i1 was enriched in membrane area of yet unidentified early mitotic cells in the VZ and the marginal zone at E14. Acute knockdown of G\u03b1i1 with <jats:italic>in utero<\/jats:italic> electroporation in cerebral cortex caused cell cycle elongation of the neural progenitor cells and promoted their cell cycle exit. G\u03b1i1\u2010deficient cortical neurons also exhibited delayed radial migration during corticogenesis, with abnormally elongated leading processes and hampered nucleokinesis. In addition, silencing of G\u03b1i1 prevented basal dendrite development. The migration and dendritic phenotypes were at least partially rescued by an RNAi\u2010resistant version of G\u03b1i1. Collectively, these results strongly suggest a crucial role of Gi1 in cortical development, and disturbance of its function may cause deficits in synaptic network formation, leading to neurodevelopmental disorders.<\/jats:p><\/jats:sec><jats:sec><jats:label\/><jats:p>\n<jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc15205-fig-0007-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text>\n<\/jats:p><\/jats:sec>","DOI":"10.1111\/jnc.15205","type":"journal-article","created":{"date-parts":[[2020,10,7]],"date-time":"2020-10-07T04:43:06Z","timestamp":1602045786000},"page":"1167-1181","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Heterotrimeric G\u2010protein, Gi1, is involved in the regulation of proliferation, 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