{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,20]],"date-time":"2025-12-20T21:53:09Z","timestamp":1766267589705,"version":"3.40.5"},"reference-count":71,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,12,5]],"date-time":"2020-12-05T00:00:00Z","timestamp":1607126400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Rarefaction of the dendritic tree leading to neuronal dysfunction is a hallmark of many neurodegenerative diseases and we have shown previously that heat shock protein B5 (HspB5)\/\u03b1B\u2010crystallin is able to increase dendritic complexity in vitro. The aim of this study was to investigate if this effect is also present in vivo, if HspB5 can counteract dendritic rarefaction under pathophysiological conditions and the impact of phosphorylation of HspB5 in this process. HspB5 and eight mutants inhibiting or mimicking phosphorylation at the three phosphorylation sites serine (S)19, S45, and S59 were over\u2010expressed in cultured rat hippocampal neurons with subsequent investigation of the complexity of the dendritic tree. Sholl analysis revealed significant higher complexity of the dendritic tree after over\u2010expression of wild\u2010type HspB5 and the mutant HspB5\u2010AEE. All other mutants showed no or minor effects. For in vivo investigation in utero electroporation of mouse embryos was applied. At embryonal day E15.5 the respective plasmids were injected, cornu ammonis 1 (CA1) pyramidal cells transfected by electroporation and their basal dendritic trees were analyzed at post\u2010natal day P15. In vivo, HspB5 and HspB5\u2010AEE led to an increase of total dendritic length as well as a higher complexity. Finally, the dendritic effect of HspB5 was investigated under a pathophysiological condition, that is, iron deficiency which reportedly results in dendritic rarefaction. HspB5 and HspB5\u2010AEE but not the non\u2010phosphorylatable mutant HspB5\u2010AAA significantly counteracted the dendritic rarefaction. Thus, our data suggest that up\u2010regulation and selective phosphorylation of HspB5 in neurodegenerative diseases may preserve dendritic morphology and counteract neuronal dysfunction.<\/jats:p><\/jats:sec><jats:sec><jats:label\/><jats:p>\n<jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc15247-fig-0007-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text>\n<\/jats:p><\/jats:sec>","DOI":"10.1111\/jnc.15247","type":"journal-article","created":{"date-parts":[[2020,11,21]],"date-time":"2020-11-21T19:25:01Z","timestamp":1605986701000},"page":"2055-2069","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["HspB5\/\u03b1B\u2010crystallin phosphorylation at S45 and S59 is essential for protection of the dendritic tree of rat hippocampal neurons"],"prefix":"10.1111","volume":"157","author":[{"given":"Britta","family":"Bartelt\u2010Kirbach","sequence":"first","affiliation":[{"name":"Institute of Anatomy and Cell Biology University of Ulm Ulm Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christoph","family":"Wiegreffe","sequence":"additional","affiliation":[{"name":"Institute of Molecular and Cellular Anatomy University of Ulm Ulm Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samuel","family":"Birk","sequence":"additional","affiliation":[{"name":"Institute of Anatomy and Cell Biology University of Ulm Ulm Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tina","family":"Baur","sequence":"additional","affiliation":[{"name":"Institute of Anatomy and Cell Biology University of Ulm Ulm Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margarethe","family":"Moron","sequence":"additional","affiliation":[{"name":"Institute of Anatomy and Cell Biology University of Ulm Ulm Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Britsch","sequence":"additional","affiliation":[{"name":"Institute of Molecular and Cellular Anatomy University of Ulm Ulm Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1942-0246","authenticated-orcid":false,"given":"Nikola","family":"Golenhofen","sequence":"additional","affiliation":[{"name":"Institute of Anatomy and Cell Biology University of Ulm Ulm Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,12,5]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_8_2_1","DOI":"10.1016\/j.bbamcr.2011.04.009"},{"doi-asserted-by":"publisher","key":"e_1_2_8_3_1","DOI":"10.1016\/j.jmb.2007.11.019"},{"doi-asserted-by":"publisher","key":"e_1_2_8_4_1","DOI":"10.1073\/pnas.1107368108"},{"doi-asserted-by":"publisher","key":"e_1_2_8_5_1","DOI":"10.1016\/j.bbagen.2015.09.017"},{"doi-asserted-by":"publisher","key":"e_1_2_8_6_1","DOI":"10.1007\/s12192-013-0452-9"},{"doi-asserted-by":"publisher","key":"e_1_2_8_7_1","DOI":"10.1007\/s00018-016-2219-9"},{"doi-asserted-by":"publisher","key":"e_1_2_8_8_1","DOI":"10.1007\/s12192-017-0794-9"},{"doi-asserted-by":"publisher","key":"e_1_2_8_9_1","DOI":"10.1111\/jth.13602"},{"doi-asserted-by":"publisher","key":"e_1_2_8_10_1","DOI":"10.1159\/000448514"},{"volume-title":"Molecular and cellular biology of the eye lens","year":"1981","author":"Bloemendal H.","key":"e_1_2_8_11_1"},{"doi-asserted-by":"publisher","key":"e_1_2_8_12_1","DOI":"10.1016\/0006-291X(87)91457-4"},{"doi-asserted-by":"publisher","key":"e_1_2_8_13_1","DOI":"10.1016\/j.bbrc.2016.09.071"},{"doi-asserted-by":"publisher","key":"e_1_2_8_14_1","DOI":"10.1016\/j.brainresbull.2013.12.004"},{"doi-asserted-by":"publisher","key":"e_1_2_8_15_1","DOI":"10.1016\/0006-8993(87)90028-X"},{"doi-asserted-by":"publisher","key":"e_1_2_8_16_1","DOI":"10.1016\/j.biocel.2012.06.012"},{"doi-asserted-by":"publisher","key":"e_1_2_8_17_1","DOI":"10.1042\/BJ20060981"},{"doi-asserted-by":"publisher","key":"e_1_2_8_18_1","DOI":"10.1242\/jcs.01021"},{"doi-asserted-by":"publisher","key":"e_1_2_8_19_1","DOI":"10.18632\/oncotarget.20366"},{"doi-asserted-by":"publisher","key":"e_1_2_8_20_1","DOI":"10.1006\/bbrc.2001.5427"},{"doi-asserted-by":"publisher","key":"e_1_2_8_21_1","DOI":"10.1006\/jmcc.2001.1513"},{"doi-asserted-by":"publisher","key":"e_1_2_8_22_1","DOI":"10.2174\/1381612822666160519113339"},{"doi-asserted-by":"publisher","key":"e_1_2_8_23_1","DOI":"10.1006\/jmcc.1998.0892"},{"doi-asserted-by":"publisher","key":"e_1_2_8_24_1","DOI":"10.1007\/s00424-005-1488-1"},{"doi-asserted-by":"publisher","key":"e_1_2_8_25_1","DOI":"10.1038\/nrm.2017.73"},{"doi-asserted-by":"publisher","key":"e_1_2_8_26_1","DOI":"10.1023\/A:1015549725819"},{"doi-asserted-by":"publisher","key":"e_1_2_8_27_1","DOI":"10.1093\/hmg\/ddp013"},{"doi-asserted-by":"publisher","key":"e_1_2_8_28_1","DOI":"10.1016\/0006-8993(91)90493-F"},{"doi-asserted-by":"publisher","key":"e_1_2_8_29_1","DOI":"10.1038\/nsmb993"},{"doi-asserted-by":"publisher","key":"e_1_2_8_30_1","DOI":"10.1016\/j.bbagen.2015.06.008"},{"doi-asserted-by":"publisher","key":"e_1_2_8_31_1","DOI":"10.1002\/jcp.1041590107"},{"doi-asserted-by":"publisher","key":"e_1_2_8_32_1","DOI":"10.1074\/jbc.M003864200"},{"doi-asserted-by":"publisher","key":"e_1_2_8_33_1","DOI":"10.1073\/pnas.89.21.10449"},{"doi-asserted-by":"publisher","key":"e_1_2_8_34_1","DOI":"10.1371\/journal.pone.0011795"},{"doi-asserted-by":"publisher","key":"e_1_2_8_35_1","DOI":"10.1007\/978-3-7091-0693-8_33"},{"doi-asserted-by":"publisher","key":"e_1_2_8_36_1","DOI":"10.1074\/jbc.M009004200"},{"doi-asserted-by":"publisher","key":"e_1_2_8_37_1","DOI":"10.1074\/jbc.272.47.29934"},{"doi-asserted-by":"publisher","key":"e_1_2_8_38_1","DOI":"10.1016\/0006-8993(94)01393-V"},{"doi-asserted-by":"publisher","key":"e_1_2_8_39_1","DOI":"10.1177\/38.1.2294148"},{"key":"e_1_2_8_40_1","first-page":"345","article-title":"Accumulation of alpha B\u2010crystallin in central nervous system glia and neurons in pathologic conditions","volume":"140","author":"Iwaki T.","year":"1992","journal-title":"American Journal of Pathology"},{"doi-asserted-by":"publisher","key":"e_1_2_8_41_1","DOI":"10.1038\/nrn2836"},{"doi-asserted-by":"publisher","key":"e_1_2_8_42_1","DOI":"10.1093\/oxfordjournals.jbchem.a124230"},{"doi-asserted-by":"publisher","key":"e_1_2_8_43_1","DOI":"10.1007\/BF00227673"},{"doi-asserted-by":"publisher","key":"e_1_2_8_44_1","DOI":"10.1002\/jnr.22536"},{"doi-asserted-by":"publisher","key":"e_1_2_8_45_1","DOI":"10.1016\/j.mcn.2012.03.005"},{"doi-asserted-by":"publisher","key":"e_1_2_8_46_1","DOI":"10.1016\/j.yexcr.2006.07.025"},{"doi-asserted-by":"publisher","key":"e_1_2_8_47_1","DOI":"10.1111\/j.1471-4159.2011.07317.x"},{"doi-asserted-by":"publisher","key":"e_1_2_8_48_1","DOI":"10.1111\/j.1365-2990.1992.tb00796.x"},{"doi-asserted-by":"publisher","key":"e_1_2_8_49_1","DOI":"10.12688\/f1000research.16038.1"},{"key":"e_1_2_8_50_1","first-page":"61","article-title":"Untersuchung der Proteinsubstanzen in den lichtbrechenden Medien des Auges","volume":"18","author":"M\u00f6rner C. T.","year":"1894","journal-title":"Hoppe Seyler S Z Physiol Chem"},{"doi-asserted-by":"publisher","key":"e_1_2_8_51_1","DOI":"10.1161\/01.RES.0000052989.83995.A5"},{"doi-asserted-by":"publisher","key":"e_1_2_8_52_1","DOI":"10.1152\/ajpheart.00715.2003"},{"doi-asserted-by":"publisher","key":"e_1_2_8_53_1","DOI":"10.1074\/jbc.M709678200"},{"doi-asserted-by":"publisher","key":"e_1_2_8_54_1","DOI":"10.1152\/physrev.00023.2010"},{"doi-asserted-by":"publisher","key":"e_1_2_8_55_1","DOI":"10.1371\/journal.pone.0016550"},{"doi-asserted-by":"publisher","key":"e_1_2_8_56_1","DOI":"10.1038\/nature05935"},{"doi-asserted-by":"publisher","key":"e_1_2_8_57_1","DOI":"10.1146\/annurev.neuro.29.051605.112907"},{"doi-asserted-by":"publisher","key":"e_1_2_8_58_1","DOI":"10.1242\/jcs.112.13.2099"},{"doi-asserted-by":"publisher","key":"e_1_2_8_59_1","DOI":"10.1073\/pnas.1308898110"},{"doi-asserted-by":"publisher","key":"e_1_2_8_60_1","DOI":"10.1016\/j.neuroscience.2008.01.058"},{"doi-asserted-by":"publisher","key":"e_1_2_8_61_1","DOI":"10.1096\/fj.00-0199com"},{"doi-asserted-by":"publisher","key":"e_1_2_8_62_1","DOI":"10.1097\/00001756-199908020-00009"},{"doi-asserted-by":"publisher","key":"e_1_2_8_63_1","DOI":"10.1007\/BF00296185"},{"doi-asserted-by":"publisher","key":"e_1_2_8_64_1","DOI":"10.1007\/s00418-012-0964-x"},{"doi-asserted-by":"publisher","key":"e_1_2_8_65_1","DOI":"10.1007\/s12192-015-0650-8"},{"doi-asserted-by":"publisher","key":"e_1_2_8_66_1","DOI":"10.1016\/j.jmb.2006.12.012"},{"doi-asserted-by":"publisher","key":"e_1_2_8_67_1","DOI":"10.1002\/ana.410300410"},{"doi-asserted-by":"publisher","key":"e_1_2_8_68_1","DOI":"10.1016\/j.biocel.2012.05.024"},{"key":"e_1_2_8_69_1","first-page":"55886","article-title":"Time\u2010lapse confocal imaging of migrating neurons in organotypic slice culture of embryonic mouse brain using in utero electroporation","volume":"125","author":"Wiegreffe C.","year":"2017","journal-title":"Journal of Visualized Experiments"},{"doi-asserted-by":"publisher","key":"e_1_2_8_70_1","DOI":"10.1016\/j.neuron.2015.06.023"},{"doi-asserted-by":"publisher","key":"e_1_2_8_71_1","DOI":"10.1111\/j.1365-2990.2006.00689.x"},{"doi-asserted-by":"publisher","key":"e_1_2_8_72_1","DOI":"10.1146\/annurev.bi.57.070188.002403"}],"container-title":["Journal of Neurochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jnc.15247","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jnc.15247","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jnc.15247","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,29]],"date-time":"2023-08-29T12:24:02Z","timestamp":1693311842000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jnc.15247"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,12,5]]},"references-count":71,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2021,6]]}},"alternative-id":["10.1111\/jnc.15247"],"URL":"https:\/\/doi.org\/10.1111\/jnc.15247","archive":["Portico"],"relation":{},"ISSN":["0022-3042","1471-4159"],"issn-type":[{"type":"print","value":"0022-3042"},{"type":"electronic","value":"1471-4159"}],"subject":[],"published":{"date-parts":[[2020,12,5]]},"assertion":[{"value":"2020-07-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-11-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}