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In several disease models, the second messenger cGMP accumulates in the degenerating photoreceptors, where it may over\u2010activate specific cGMP\u2010interacting proteins, like cGMP\u2010dependent protein kinase. Moreover, interventions that counteract the activity of these proteins lead to reduced photoreceptor cell death. Yet there is little or no information whether other than such regular cGMP\u2010interactors are present in the retina, which we, therefore, investigated in wild\u2010type and retinal degeneration (<jats:italic>rd1<\/jats:italic>, <jats:italic>rd10<\/jats:italic>, and <jats:italic>rd2<\/jats:italic>) mouse models. An affinity chromatography based proteomics approach that utilized immobilized cGMP analogs was applied to enrich and select for regular and potentially new cGMP\u2010interacting proteins as identified by mass spectrometry. This approach revealed 12 regular and 10 potentially new retinal cGMP\u2010interacting proteins (e.g., EPAC2 and CaMKII<jats:italic>\u03b1<\/jats:italic>). Several of the latter were found to be expressed in the photoreceptors and to have proximity to cGMP and may thus be of interest when defining prospective therapeutic targets or biomarkers for retinal degeneration.<\/jats:p><\/jats:sec><jats:sec><jats:label\/><jats:p>\n<jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc15251-fig-0005-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text>\n<\/jats:p><\/jats:sec>","DOI":"10.1111\/jnc.15251","type":"journal-article","created":{"date-parts":[[2020,11,24]],"date-time":"2020-11-24T08:55:38Z","timestamp":1606208138000},"page":"2173-2186","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["The cGMP system in normal and degenerating mouse neuroretina: New proteins with cGMP interaction potential identified by a proteomics approach"],"prefix":"10.1111","volume":"157","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4932-8456","authenticated-orcid":false,"given":"Michel","family":"Rasmussen","sequence":"first","affiliation":[{"name":"Faculty of Medicine Department of Clinical Sciences Lund Lund University Ophthalmology, Lund Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charlotte","family":"Welinder","sequence":"additional","affiliation":[{"name":"Faculty of Medicine Department of Clinical Sciences Lund, Oncology Lund University Lund Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank","family":"Schwede","sequence":"additional","affiliation":[{"name":"BIOLOG Life Science Institute GmbH &amp; Co. KG Bremen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Per","family":"Ekstr\u00f6m","sequence":"additional","affiliation":[{"name":"Faculty of Medicine Department of Clinical Sciences Lund Lund University Ophthalmology, Lund Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,12,5]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0112142"},{"key":"e_1_2_9_3_1","first-page":"115","article-title":"Expression of beta subunit 2 of Ca 2+ \/calmodulin\u2010dependent protein kinase I in the developing rat retina","volume":"61","author":"Aulia J. 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