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Ng has been suggested to bind to calmodulin and phosphatidic acid via its centrally located IQ domain. Ng is cleaved within this functionally important domain, yielding the majority of fragments identified in cerebrospinal fluid (CSF), suggesting that cleavage of Ng may be a mechanism to regulate its function. Up to now, Ng has been shown to be present in CSF as both C\u2010terminal fragments as well as full\u2010length protein. To obtain an overview of the different molecular forms of Ng present in CSF, we show by size exclusion chromatography (SEC), immunoblotting, immunoprecipitation, and MS that Ng is present in CSF as several molecular forms. Besides monomeric full\u2010length Ng, also higher molecular weight forms of Ng, and C\u2010terminal\u2010 and previously not identified N\u2010terminal fragments were observed. We found by immunodepletion that C\u2010terminal peptides contribute on average to ~50% of the total\u2010Ng ELISA signal in CSF samples. There were no differences in the overall C\u2010terminal fragment\/total\u2010Ng ratios between samples from AD and control groups. In addition, we found that monomeric Ng and its C\u2010terminal fragments bind to heparin via a heparin\u2010binding motif, which might be of relevance for their export mechanism from neurons. Taken together, this study highlights the presence of several molecular forms of Ng in CSF, comprising monomeric full\u2010length Ng, and N\u2010 and C\u2010terminal truncations of Ng, as well as larger forms of still unknown composition.<\/jats:p><\/jats:sec><jats:sec><jats:label\/><jats:p>\n<jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc15252-fig-0009-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text>\n<\/jats:p><\/jats:sec>","DOI":"10.1111\/jnc.15252","type":"journal-article","created":{"date-parts":[[2020,11,29]],"date-time":"2020-11-29T09:25:15Z","timestamp":1606641915000},"page":"816-833","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Molecular forms of neurogranin in cerebrospinal 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