{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,27]],"date-time":"2025-12-27T07:14:04Z","timestamp":1766819644579,"version":"3.40.5"},"reference-count":52,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,2,2]],"date-time":"2021-02-02T00:00:00Z","timestamp":1612224000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["310030_173222"],"award-info":[{"award-number":["310030_173222"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Brain metabolism evolves rapidly during early post\u2010natal development in the rat. While changes in amino acids, energy metabolites, antioxidants or metabolites involved in phospholipid metabolism have been reported in the early stages, neurometabolic changes during the later post\u2010natal period are less well characterized. Therefore, we aimed to assess the neurometabolic changes in male Wistar rats between post\u2010natal days 29 and 77 (p29\u2010p77) using longitudinal magnetic resonance spectroscopy (MRS) in vivo at 9.4 Tesla. <jats:sup>1<\/jats:sup>H MRS was performed in the hippocampus between p29 and p77 at 1\u2010week intervals (<jats:italic>n<\/jats:italic>\u00a0=\u00a07) and in the cerebellum between p35 and p77 at 2\u2010week intervals (<jats:italic>n<\/jats:italic>\u00a0=\u00a07) using the SPECIAL sequence at ultra\u2010short echo\u2010time. NOE enhanced and <jats:sup>1<\/jats:sup>H decoupled <jats:sup>31<\/jats:sup>P MR spectra were acquired at p35, p48 and p63 (<jats:italic>n<\/jats:italic>\u00a0=\u00a07) in a larger voxel covering cortex, hippocampus and part of the striatum. The hippocampus showed a decrease in taurine concentration and an increase in glutamate (with more pronounced changes until p49), seemingly a continuation of their well\u2010described changes in the early post\u2010natal period. A constant increase in myo\u2010inositol and choline\u2010containing compounds in the hippocampus (in particular glycero\u2010phosphocholine as shown by <jats:sup>31<\/jats:sup>P MRS) was measured throughout the observation period, probably related to membrane metabolism and myelination. The cerebellum showed only a significant increase in myo\u2010inositol between p35 and p77. In conclusion, this study showed important changes in brain metabolites in both the hippocampus and cerebellum in the later post\u2010natal period (p29\/p35\u2010p77) of male rats, something previously unreported. Based on these novel data, changes in some neurometabolites beyond p28\u201035, conventionally accepted as the cut off for adulthood, should be taken into account in both experimental design and data interpretation in this animal model.<\/jats:p><\/jats:sec><jats:sec><jats:label\/><jats:p>\n<jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc15294-fig-0007-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text>\n<\/jats:p><\/jats:sec>","DOI":"10.1111\/jnc.15294","type":"journal-article","created":{"date-parts":[[2021,1,15]],"date-time":"2021-01-15T01:16:55Z","timestamp":1610673415000},"page":"508-519","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Late post\u2010natal neurometabolic development in healthy male rats using <sup>1<\/sup>H and <sup>31<\/sup>P magnetic resonance spectroscopy"],"prefix":"10.1111","volume":"157","author":[{"given":"Veronika","family":"Ra\u010dkayov\u00e1","sequence":"first","affiliation":[{"name":"CIBM Center for Biomedical Imaging Lausanne Switzerland"},{"name":"Animal Imaging and Technology Ecole Polytechnique Federale de Lausanne (EPFL) Lausanne Switzerland"},{"name":"Laboratory of Functional and Metabolic Imaging (LIFMET) Ecole Polytechnique Federale de Lausanne (EPFL) Lausanne Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dunja","family":"Simicic","sequence":"additional","affiliation":[{"name":"CIBM Center for Biomedical Imaging Lausanne Switzerland"},{"name":"Animal Imaging and Technology Ecole Polytechnique Federale de Lausanne (EPFL) Lausanne Switzerland"},{"name":"Laboratory of Functional and Metabolic Imaging (LIFMET) Ecole Polytechnique Federale de Lausanne (EPFL) Lausanne Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guillaume","family":"Donati","sequence":"additional","affiliation":[{"name":"Laboratory of Functional and Metabolic Imaging (LIFMET) Ecole Polytechnique Federale de Lausanne (EPFL) Lausanne Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8068-3344","authenticated-orcid":false,"given":"Olivier","family":"Braissant","sequence":"additional","affiliation":[{"name":"Service of Clinical Chemistry University of Lausanne and University Hospital of Lausanne Lausanne Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1870-4025","authenticated-orcid":false,"given":"Rolf","family":"Gruetter","sequence":"additional","affiliation":[{"name":"Laboratory of Functional and Metabolic Imaging (LIFMET) Ecole Polytechnique Federale de Lausanne (EPFL) Lausanne Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0711-687X","authenticated-orcid":false,"given":"Val\u00e9rie A.","family":"McLin","sequence":"additional","affiliation":[{"name":"Swiss Pediatric Liver Center University Hospitals Geneva Geneva Switzerland"},{"name":"Department of Pediatrics, Gynecology, and Obstetrics University of Geneva Medical School Geneva Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4582-2465","authenticated-orcid":false,"given":"Cristina","family":"Cudalbu","sequence":"additional","affiliation":[{"name":"CIBM Center for Biomedical Imaging Lausanne Switzerland"},{"name":"Animal Imaging and Technology Ecole Polytechnique Federale de Lausanne (EPFL) Lausanne Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,2,2]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1220759"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901450305"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901450403"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901450402"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901240303"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901260302"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0804650106"},{"key":"e_1_2_9_9_1","first-page":"83","article-title":"Timetables of neurogenesis in the human brain based on experimentally determined patterns in the rat","volume":"14","author":"Bayer S. 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