{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T18:24:27Z","timestamp":1787163867077,"version":"build-2736575974"},"reference-count":51,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,6,20]],"date-time":"2021-06-20T00:00:00Z","timestamp":1624147200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81571079"],"award-info":[{"award-number":["81571079"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>Emerging evidence indicates the early growth response 1 (Egr1) plays an important role in the pathogenesis of chronic pain. However, the regulation of Egr1 expression in the DRG and spinal cord in neuropathic pain remains unclear. In the current study, the neuropathic pain was conducted by lumber 5 spinal nerve ligation (SNL) in rats. The role of miR\u2010124\u20103p in Egr1 expression was examined. Our results showed that the SNL led to a significant increase in the expression of Egr1 mRNA and protein in the DRG and dorsal horn. This increased expression of Egr1 correlated with a reduction of miR\u2010124\u20103p in the same region. Prior i.t. injection of Egr1 decoy AYX1 inhibited the expression of Egr1 and attenuated the neuropathic pain\u2010like hypersensitivity following SNL. The dual\u2010luciferase reporter assay revealed the luciferase activity of the Egr1 3\u2032\u2010UTR plasmid was inhibited by the miR\u2010124\u20103p agomir. But this inhibition was completely reversed in the mutant 3\u2032\u2010UTR Egr1 group. In vivo, the SNL\u2010induced behavioral signs of neuropathic pain and the increases in Egr1 mRNA and protein in the DRG and dorsal horn were prevented by prior to i.t. injection of miR\u2010124\u20103p agomir. While, i.t. injection of miR\u2010124\u20103p antagomir in na\u00efve rats resulted in mechanical allodynia and thermal hyperalgesia and an overexpression of Egr1 in the DRG and dorsal horn. Together, our results suggest that the miR\u2010124\u20103p\u2010regulated Egr1 expression in the DRG and dorsal horn contributes to the development of neuropathic pain. Targeting miR\u2010124\u20103p might be a promising therapeutic strategy in the treatment of chronic pain.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      <jats:boxed-text content-type=\"graphic\" position=\"anchor\">\n                        <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc15433-fig-0010-m.png\">\n                          <jats:alt-text>image<\/jats:alt-text>\n                        <\/jats:graphic>\n                      <\/jats:boxed-text>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jnc.15433","type":"journal-article","created":{"date-parts":[[2021,5,19]],"date-time":"2021-05-19T00:01:29Z","timestamp":1621382489000},"page":"928-942","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["MicroRNA\u2010124\u20103p attenuates the development of nerve injury\u2013induced neuropathic pain by targeting early growth response 1 in the dorsal root ganglia and spinal dorsal horn"],"prefix":"10.1111","volume":"158","author":[{"given":"Mingjun","family":"Jiang","sequence":"first","affiliation":[{"name":"Department of Physiology and Neurobiology School of Basic Medical Sciences Zhengzhou University Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Physiology and Neurobiology School of Basic Medical Sciences Zhengzhou University Zhengzhou 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