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Glucose uptake is mediated by specific transporters whose regulation and expression are critical for the pathogenesis of many diseases, including diabetes and diabetic retinopathy. Here, we used immunofluorescence to show that glucose transporter\u20102 (GLUT2) is expressed in horizontal cells of the mouse neuroretina in proximity to inner retinal capillaries. To study the function of GLUT2 in the murine retina, we used organotypic retinal explants, cultivated under entirely controlled, serum\u2010free conditions and exposed them to streptozotocin, a cytotoxic drug transported exclusively by GLUT2. Contrary to our expectations, streptozotocin did not measurably affect horizontal cell viability, while it ablated rod and cone photoreceptors in a concentration\u2010dependent manner. Staining for poly\u2010ADP\u2010ribose (PAR) indicated that the detrimental effect of streptozotocin on photoreceptors may be associated with DNA damage. The negative effect of streptozotocin on the viability of rod photoreceptors was counteracted by co\u2010administration of either the inhibitor of connexin\u2010formed hemi\u2010channels meclofenamic acid or the blocker of clathrin\u2010mediated endocytosis dynasore. Remarkably, cone photoreceptors were not protected from streptozotocin\u2010induced degeneration by neither of the two drugs. Overall, these data suggest the existence of a GLUT2\u2010dependent glucose transport shuttle, from horizontal cells into photoreceptor synapses. Moreover, our study points at different glucose uptake mechanisms in rod and cone photoreceptors.<\/jats:p><\/jats:sec><jats:sec><jats:label\/><jats:p><jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc15533-fig-0008-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text><\/jats:p><\/jats:sec>","DOI":"10.1111\/jnc.15533","type":"journal-article","created":{"date-parts":[[2021,11,2]],"date-time":"2021-11-02T21:05:06Z","timestamp":1635887106000},"page":"283-296","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Expression of glucose transporter\u20102 in murine retina: Evidence for glucose transport from horizontal cells to photoreceptor synapses"],"prefix":"10.1111","volume":"160","author":[{"given":"Ming","family":"Yang","sequence":"first","affiliation":[{"name":"Affiliated Hospital of Yunnan University &amp; 2nd People\u2019s Hospital of Yunnan Province Kunming China"},{"name":"Yunnan Eye Institute &amp; Key Laboratory of Yunnan Province Kunming China"},{"name":"1st Affiliated Hospital of Kunming Medical University Kunming China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9007-4227","authenticated-orcid":false,"given":"Yiyi","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute for Ophthalmic Research Eberhard\u2010Karls\u2010Universit\u00e4t T\u00fcbingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9501-0695","authenticated-orcid":false,"given":"Stavros","family":"Vagionitis","sequence":"additional","affiliation":[{"name":"Institute of Neuronal Cell Biology Technical University of Munich Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"El\u00f6d","family":"K\u00f6rtvely","sequence":"additional","affiliation":[{"name":"Roche Pharma Research and Early Development Immunology, Infectious Diseases and Ophthalmology (I2O) Discovery and Translational Area Roche Innovation Center Basel, F. Hoffmann\u2010La Roche Ltd Basel Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marius","family":"Ueffing","sequence":"additional","affiliation":[{"name":"Institute for Ophthalmic Research Eberhard\u2010Karls\u2010Universit\u00e4t T\u00fcbingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oliver","family":"Schmachtenberg","sequence":"additional","affiliation":[{"name":"CINV Instituto de Biolog\u00eda Facultad de Ciencias Universidad de Valpara\u00edso Valpara\u00edso Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhulin","family":"Hu","sequence":"additional","affiliation":[{"name":"Affiliated Hospital of Yunnan University &amp; 2nd People\u2019s Hospital of Yunnan Province Kunming China"},{"name":"Yunnan Eye Institute &amp; Key Laboratory of Yunnan Province Kunming China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3202-5993","authenticated-orcid":false,"given":"Kangwei","family":"Jiao","sequence":"additional","affiliation":[{"name":"Affiliated Hospital of Yunnan University &amp; 2nd People\u2019s Hospital of Yunnan Province Kunming China"},{"name":"Yunnan Eye Institute &amp; Key Laboratory of Yunnan Province Kunming China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7355-5742","authenticated-orcid":false,"given":"Fran\u00e7ois","family":"Paquet\u2010Durand","sequence":"additional","affiliation":[{"name":"Institute for Ophthalmic Research Eberhard\u2010Karls\u2010Universit\u00e4t T\u00fcbingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,11,14]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.03.023"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1005073"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.12\u201010927"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.3791\/61868"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.2337\/diacare.13.3.198"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmet.2017.04.010"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/347677a0"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.2337\/diab.18.9.606"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0891\u20100618(01)00140\u20105"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.eye.6700133"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.24805"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.2511"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2012.03.018"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471141755.ph0547s70"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010med\u2010061813\u2010012908"},{"key":"e_1_2_11_17_1","first-page":"1033","article-title":"Fenamates: A novel class of reversible gap junction blockers","volume":"298","author":"Harks E. 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