{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T18:40:23Z","timestamp":1790707223457,"version":"4.1.0"},"reference-count":41,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,11,18]],"date-time":"2021-11-18T00:00:00Z","timestamp":1637193600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82171213"],"award-info":[{"award-number":["82171213"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      Trigeminal neuralgia (TN) is a type of severe paroxysmal neuropathic pain commonly triggered by mild mechanical stimulation in the orofacial area. Piezo2, a mechanically gated ion channel that mediates tactile allodynia in neuropathic pain, can be potentiated by a cyclic adenosine monophosphate (cAMP)\u2010dependent signaling pathway that involves the exchange protein directly activated by cAMP 1 (Epac1). To study whether Piezo2\u2010mediated mechanotransduction contributes to peripheral sensitization in a rat model of TN after trigeminal nerve compression injury, the expression of Piezo2 and activation of cAMP signal\u2010related molecules in the trigeminal ganglion (TG) were detected. Changes in purinergic P2 receptors in the TG were also studied by RNA\u2010seq. The expression of Piezo2, cAMP, and Epac1 in the TG of the TN animals increased after chronic compression of the trigeminal nerve root (CCT) for 21\u00a0days, but Piezo2 knockdown by shRNA in the TG attenuated orofacial mechanical allodynia. Purinergic P2 receptors P2X4, P2X7, P2Y1, and P2Y2 were significantly up\u2010regulated after CCT injury. In vitro, Piezo2 expression in TG neurons was significantly increased by exogenous adenosine 5'\u2010triphosphate (ATP) and Ca\n                      <jats:sup>2+<\/jats:sup>\n                      ionophore ionomycin. ATP pre\u2010treated TG neurons displayed elevated [Ca\n                      <jats:sup>2+<\/jats:sup>\n                      ]\n                      <jats:sub>i<\/jats:sub>\n                      and faster increase in responding to blockage of Na\n                      <jats:sup>+<\/jats:sup>\n                      \/Ca\n                      <jats:sup>2+<\/jats:sup>\n                      exchanger by KB\u2010R7943. Furthermore, mechanical stimulation of cultured TG neurons led to sustained elevation in [Ca\n                      <jats:sup>2+<\/jats:sup>\n                      ]\n                      <jats:sub>i<\/jats:sub>\n                      in ATP pre\u2010treated TG neurons, which is much less in na\u00efve TG neurons, or is significantly reduced by Piezo2 inhibitor GsMTx4. These results indicated a pivotal role of Piezo2 in peripheral mechanical allodynia in the rat CCT model. Extracellular ATP, Ca\n                      <jats:sup>2+<\/jats:sup>\n                      influx, and the cAMP\u2010to\u2010Epac1 signaling pathway synergistically contribute to the pathogenesis and the persistence of mechanical allodynia.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      <jats:boxed-text content-type=\"graphic\" position=\"anchor\">\n                        <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc15537-fig-0011-m.png\">\n                          <jats:alt-text>image<\/jats:alt-text>\n                        <\/jats:graphic>\n                      <\/jats:boxed-text>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jnc.15537","type":"journal-article","created":{"date-parts":[[2021,11,10]],"date-time":"2021-11-10T11:25:00Z","timestamp":1636543500000},"page":"376-391","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Extracellular ATP and cAMP signaling promote Piezo2\u2010dependent mechanical allodynia after trigeminal nerve compression injury"],"prefix":"10.1111","volume":"160","author":[{"given":"Zhaoke","family":"Luo","sequence":"first","affiliation":[{"name":"Key Laboratory of Brain Aging and Neurodegenerative Diseases of Fujian Province Laboratory of Clinical Applied Anatomy the School of Basic Medical Sciences Fujian Medical University  Fuzhou China"},{"name":"Department of Human Anatomy the School of Basic Medical Sciences Fujian Medical University  Fuzhou 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