{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:14:16Z","timestamp":1787015656803,"version":"build-2736575974"},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,3,9]],"date-time":"2023-03-09T00:00:00Z","timestamp":1678320000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP22ek0109431"],"award-info":[{"award-number":["JP22ek0109431"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007449","name":"Takeda Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007449","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008732","name":"Uehara Memorial Foundation","doi-asserted-by":"publisher","award":["no. 201810115"],"award-info":[{"award-number":["no. 201810115"]}],"id":[{"id":"10.13039\/100008732","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2023,4]]},"abstract":"Abstract<\/jats:title>\n \n Dopamine (DA) is involved in neurological and physiological functions such as motor control. L\u20103,4\u2010dihydroxyphenylalanine (L\u2010DOPA), a precursor of DA, is conventionally believed to be an inert amino acid precursor of DA, and its major therapeutic effects in Parkinson's disease (PD) are mediated through its conversion to DA. On the contrary, accumulating evidence suggests that L\u2010DOPA itself is a neurotransmitter. We here show that L\u2010DOPA potentiates DA D2 receptor (DRD2) signaling through GPR143, the gene product of X\u2010linked ocular albinism 1, a G\u2010protein\u2010coupled receptor for L\u2010DOPA. In\n Gpr143<\/jats:italic>\n \u2010gene\u2010deficient (\n Gpr143<\/jats:italic>\n \n \u2212\/y<\/jats:italic>\n <\/jats:sup>\n ) mice, quinpirole, a DRD2\/DRD3 agonist, \u2010induced hypolocomotion was attenuated compared to wild\u2010type (\n WT<\/jats:italic>\n ) mice. Administration of non\u2010effective dose of L\u2010DOPA methyl ester augmented the quinpirole\u2010induced hypolocomotion in\n WT<\/jats:italic>\n mice but not in\n Gpr143<\/jats:italic>\n \n \u2212\/y<\/jats:italic>\n <\/jats:sup>\n mice. In cells co\u2010expressing GPR143 and DRD2, L\u2010DOPA enhanced the interaction between GPR143 and DRD2 and augmented quinpirole\u2010induced decrease in cAMP levels. This augmentation by L\u2010DOPA was not observed in cells co\u2010expressing GPR143 and DRD1 or DRD3. Chimeric analysis in which the domain of GPR143 was replaced with GPR37 revealed that GPR143 interacted with DRD2 at the fifth transmembrane domain. Intracerebroventricular administration of a peptide that disrupted the interaction mitigated quinpirole\u2010induced behavioral changes in\n WT<\/jats:italic>\n mice but not in\n Gpr143<\/jats:italic>\n \n \u2212\/y<\/jats:italic>\n <\/jats:sup>\n mice. These findings provide evidence that coupling between GPR143 and DRD2 is required for selective DRD2 modulation by L\u2010DOPA in the dorsal striatum.\n \n \n image<\/jats:alt-text>\n <\/jats:graphic>\n <\/jats:boxed-text>\n <\/jats:p>","DOI":"10.1111\/jnc.15789","type":"journal-article","created":{"date-parts":[[2023,2,20]],"date-time":"2023-02-20T08:59:21Z","timestamp":1676883561000},"page":"177-195","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Coupling between\n GPR143<\/scp>\n and dopamine\n D2<\/scp>\n receptor is required for selective potentiation of dopamine\n D2<\/scp>\n receptor function by L\u20103,4\u2010dihydroxyphenylalanine in the dorsal striatum"],"prefix":"10.1111","volume":"165","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3094-0058","authenticated-orcid":false,"given":"Daiki","family":"Masukawa","sequence":"first","affiliation":[{"name":"Department of Molecular Pharmacology and 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