{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T14:20:48Z","timestamp":1787235648626,"version":"build-2736575974"},"reference-count":55,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,5,19]],"date-time":"2023-05-19T00:00:00Z","timestamp":1684454400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81072465"],"award-info":[{"award-number":["81072465"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81571579"],"award-info":[{"award-number":["81571579"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Myasthenia gravis (MG) is an antibody\u2010mediated autoimmune disorder characterized by fluctuation of fatigue and weakness of muscle. Due to the heterogeneity of the course of MG, available biomarkers for prognostic prediction are urgently needed. Ceramide (Cer) was reported to participate in immune regulation and many autoimmune diseases, but its effects on MG remain undefined. This study aimed to investigate the ceramides expression levels in MG patients and their potential as novel biomarkers of disease severity. Levels of plasma ceramides were determined by ultra performance liquid chromatography\u2013tandem mass spectrometry (UPLC\u2010MS\/MS). Severity of disease was assessed by quantitative MG scores (QMGs), MG\u2010specific activities of daily living scale (MG\u2010ADLs) and 15\u2010item MG quality of Life (MG\u2010QOL15). The concentrations of serum interleukin\u20101\u03b2 (IL\u20101\u03b2), IL\u20106, IL\u201017A, and IL\u201021 were determined by enzyme\u2010linked immunosorbent assay (ELISA), and the proportions of circulating memory B cells and plasmablasts were detected by flow\u2010cytometry assay. Four plasma ceramides levels we studied were detected higher in MG patients. And three of them (C16:0\u2010Cer, C18:0\u2010Cer, and C24:0\u2010Cer) were positively associated with QMGs. In addition, receiver operating characteristic (ROC) analysis suggested that plasma ceramides have a good ability of differentiating MG from HCs. Importantly, only C18:0\u2010Cer was shown to be positively associated with the concentration of serum IL and circulating memory B cells, and the decrease in plasma C18:0\u2010Cer paralleled the clinical improvement of patients with MG. All together, our data suggest that ceramides may play an important role in the immunopathological mechanism of MG, and C18:0\u2010Cer has the potential to be a novel biomarker for disease severity in MG.\n                    <jats:boxed-text content-type=\"graphic\" position=\"anchor\">\n                      <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc15837-fig-0008-m.png\">\n                        <jats:alt-text>image<\/jats:alt-text>\n                      <\/jats:graphic>\n                    <\/jats:boxed-text>\n                  <\/jats:p>","DOI":"10.1111\/jnc.15837","type":"journal-article","created":{"date-parts":[[2023,5,10]],"date-time":"2023-05-10T14:29:46Z","timestamp":1683728986000},"page":"907-919","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Plasma\n                    <scp>C18<\/scp>\n                    :0\u2010ceramide is a novel potential biomarker for disease severity in myasthenia gravis"],"prefix":"10.1111","volume":"165","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-6253-6167","authenticated-orcid":false,"given":"Zhouao","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Neurology Affiliated Hospital of Xuzhou Medical University  Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyu","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Neurology Affiliated Hospital of Xuzhou Medical University  Xuzhou China"},{"name":"Department of Neurology, Tianjin Neurological Institute Tianjin Medical University General Hospital  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Du","sequence":"additional","affiliation":[{"name":"Department of Neurology Affiliated Hospital of Xuzhou Medical University  Xuzhou 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