{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T05:12:10Z","timestamp":1790313130778,"version":"4.1.0"},"reference-count":79,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,6,16]],"date-time":"2023-06-16T00:00:00Z","timestamp":1686873600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda","doi-asserted-by":"publisher","award":["47514"],"award-info":[{"award-number":["47514"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda","doi-asserted-by":"publisher","award":["A1\u2010S\u201017357"],"award-info":[{"award-number":["A1\u2010S\u201017357"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Ischemic stroke is a leading cause of disability worldwide. There is no simple treatment to alleviate ischemic brain injury, as thrombolytic therapy is applicable within a narrow time window. During the last years, the ketogenic diet (KD) and the exogenous administration of the ketone body \u03b2\u2010hydroxybutyrate (BHB) have been proposed as therapeutic tools for acute neurological disorders and both can reduce ischemic brain injury. However, the mechanisms involved are not completely clear. We have previously shown that the D enantiomer of BHB stimulates the autophagic flux in cultured neurons exposed to glucose deprivation (GD) and in the brain of hypoglycemic rats. Here, we have investigated the effect of the systemic administration of D\u2010BHB, followed by its continuous infusion after middle cerebral artery occlusion (MCAO), on the autophagy\u2010lysosomal pathway and the activation of the unfolded protein response (UPR). Results show for the first time that the protective effect of BHB against MCAO injury is enantiomer selective as only D\u2010BHB, the physiologic enantiomer of BHB, significantly reduced brain injury. D\u2010BHB treatment prevented the cleavage of the lysosomal membrane protein LAMP2 and stimulated the autophagic flux in the ischemic core and the penumbra. In addition, D\u2010BHB notably reduced the activation of the PERK\/eIF2\u03b1\/ATF4 pathway of the UPR and inhibited IRE1\u03b1 phosphorylation. L\u2010BHB showed no significant effect relative to ischemic animals. In cortical cultures under GD, D\u2010BHB prevented LAMP2 cleavage and decreased lysosomal number. It also abated the activation of the PERK\/eIF2\u03b1\/ATF4 pathway, partially sustained protein synthesis, and reduced pIRE1\u03b1. In contrast, L\u2010BHB showed no significant effects. Results suggest that protection elicited by D\u2010BHB treatment post\u2010ischemia prevents lysosomal rupture allowing functional autophagy, preventing the loss of proteostasis and UPR activation.<jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc15852-fig-0011-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text><\/jats:p>","DOI":"10.1111\/jnc.15852","type":"journal-article","created":{"date-parts":[[2023,6,17]],"date-time":"2023-06-17T03:44:04Z","timestamp":1686973444000},"page":"87-106","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Modulation of the autophagy\u2010lysosomal pathway and endoplasmic reticulum stress by ketone bodies in experimental models of stroke"],"prefix":"10.1111","volume":"166","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4548-4718","authenticated-orcid":false,"given":"Teresa","family":"Montiel","sequence":"first","affiliation":[{"name":"Departamento de Neuropatolog\u00eda Molecular Divisi\u00f3n de Neurociencias Instituto de Fisiolog\u00eda Celular Universidad Nacional Aut\u00f3noma de M\u00e9xico  Ciudad de M\u00e9xico M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6081-3981","authenticated-orcid":false,"given":"Juan Carlos","family":"G\u00f3mora\u2010Garc\u00eda","sequence":"additional","affiliation":[{"name":"Departamento de Neuropatolog\u00eda Molecular Divisi\u00f3n de Neurociencias Instituto de Fisiolog\u00eda Celular Universidad Nacional Aut\u00f3noma de M\u00e9xico  Ciudad de M\u00e9xico 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