{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T22:36:33Z","timestamp":1791326193326,"version":"4.3.1"},"reference-count":63,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,6,23]],"date-time":"2023-06-23T00:00:00Z","timestamp":1687478400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Basic Research Program of China","doi-asserted-by":"publisher","award":["2021YFC2501400"],"award-info":[{"award-number":["2021YFC2501400"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Basic Research Program of China","doi-asserted-by":"publisher","award":["2020YFC2005300"],"award-info":[{"award-number":["2020YFC2005300"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31971110"],"award-info":[{"award-number":["31971110"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32171142"],"award-info":[{"award-number":["32171142"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82071491"],"award-info":[{"award-number":["82071491"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31871072"],"award-info":[{"award-number":["31871072"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["8202010801"],"award-info":[{"award-number":["8202010801"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011827","name":"Guangxi Medical University","doi-asserted-by":"publisher","award":["KLLAD202201"],"award-info":[{"award-number":["KLLAD202201"]}],"id":[{"id":"10.13039\/501100011827","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The cholinergic neurons in the nucleus basalis of Meynert (NBM) are a key structure in cognition, the dysfunction of which is associated with various neurological disorders, especially dementias. However, the whole\u2010brain neural connectivity to cholinergic neurons in the NBM remains to be further and comprehensively researched. Using virus\u2010based, specific, retrograde, and anterograde tracing, we illustrated the monosynaptic inputs and axon projections of NBM cholinergic neurons in choline acetyltransferase (ChAT)\u2010Cre transgenic mice. Our results showed that NBM cholinergic neurons received mainly inputs from the caudate putamen and the posterior limb of the anterior commissure in the subcortex. Moreover, the majority of cholinergic terminals from the NBM were observed in the cortex mantle, including the motor cortex, sensory cortex, and visual cortex. Interestingly, although NBM cholinergic neurons received input projections from the caudate putamen, interstitial nucleus of the posterior limb of the anterior commissure, and central amygdaloid nucleus, NBM cholinergic neurons sparsely sent axon projection to innervate these areas. Furthermore, primary motor cortex, secondary motor cortex, and primary somatosensory cortex received abundant inputs from the NBM but sent few outputs to the NBM. Taken together, our results reveal the detailed and specific connectivity of cholinergic neurons of the NBM and provide a neuroanatomic foundation for further studies to explore the important physiological functions of NBM cholinergic neurons.<jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc15873-fig-0009-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text><\/jats:p>","DOI":"10.1111\/jnc.15873","type":"journal-article","created":{"date-parts":[[2023,6,25]],"date-time":"2023-06-25T01:21:20Z","timestamp":1687656080000},"page":"233-247","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Whole\u2010brain neural connectivity to cholinergic neurons in the nucleus basalis of Meynert"],"prefix":"10.1111","volume":"166","author":[{"given":"Zhao\u2010yi","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Anatomy and Histoembryology, School of Basic Medical Sciences Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao\u2010lian","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Histoembryology, School of Basic Medical Sciences Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mai","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Histoembryology, School of Basic Medical Sciences Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Histoembryology, School of Basic Medical Sciences Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei\u2010min","family":"Qu","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, School of Basic Medical Sciences Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9359-1150","authenticated-orcid":false,"given":"Zhi\u2010li","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, School of Basic Medical Sciences Fudan University  Shanghai China"},{"name":"State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Institutes of Brain Science Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2365-8756","authenticated-orcid":false,"given":"Xiang\u2010shan","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Histoembryology, School of Basic Medical Sciences Fudan University  Shanghai China"},{"name":"State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Institutes of Brain Science Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,6,23]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurobiolaging.2022.04.001"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7046051"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903350407"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1523\/jneurosci.08\u201011\u201004007.1988"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/cercor\/bhw195"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/npp.2016.13"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/ejn.12822"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.3233\/JAD\u2010201141"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2019.00375"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.13214"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2021.12.026"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neubiorev.2013.09.003"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaneurol.2017.3762"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awx333"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903290403"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopsych.2011.06.019"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12264\u2010020\u201000468\u20109"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnana.2016.00098"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.172.3983.601"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.parkreldis.2018.02.003"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006\u20108993(94)90950\u20104"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0079\u20106123(06)60007\u20101"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.3233\/JAD\u2010190919"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.1359"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awac093"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401\u2010015\u20101392\u20105"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2018.04.026"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010018\u201003889\u20103"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2019.05.015"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2012.04.027"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.1999.277.5.R1488"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1523\/jneurosci.22\u201005\u201001905.2002"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902140206"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306\u20104522(84)90163\u20105"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306\u20104522(83)90108\u20102"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306\u20104522(92)90156\u2010v"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.10382"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306\u20104522(95)00582\u2010x"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010017\u201000781\u20104"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010022\u201032558\u20109"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-328X(00)00194-7"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abb0556"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2011.01.003"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2014.07.002"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2016.02.032"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.1093\/cercor\/bhu241"},{"key":"e_1_2_12_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306\u20104522(95)00523\u20104"},{"key":"e_1_2_12_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902220302"},{"key":"e_1_2_12_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306\u20104522(84)90286\u20100"},{"key":"e_1_2_12_51_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.06412"},{"key":"e_1_2_12_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature14179"},{"key":"e_1_2_12_53_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awy072"},{"key":"e_1_2_12_54_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awab372"},{"key":"e_1_2_12_55_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903580108"},{"key":"e_1_2_12_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0306\u20104522(98)00202\u20104"},{"key":"e_1_2_12_57_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035\u2010020\u201002116\u20109"},{"key":"e_1_2_12_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304\u20103940(85)90048\u20105"},{"key":"e_1_2_12_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2021.113784"},{"key":"e_1_2_12_60_1","doi-asserted-by":"publisher","DOI":"10.1006\/nlme.1996.3757"},{"key":"e_1_2_12_61_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.4143"},{"key":"e_1_2_12_62_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.29055"},{"key":"e_1_2_12_63_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2018.00807"},{"key":"e_1_2_12_64_1","doi-asserted-by":"publisher","DOI":"10.1038\/lsa.2016.231"}],"container-title":["Journal of Neurochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jnc.15873","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,16]],"date-time":"2023-08-16T20:07:13Z","timestamp":1692216433000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jnc.15873"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,23]]},"references-count":63,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2023,7]]}},"alternative-id":["10.1111\/jnc.15873"],"URL":"https:\/\/doi.org\/10.1111\/jnc.15873","archive":["Portico"],"relation":{},"ISSN":["0022-3042","1471-4159"],"issn-type":[{"value":"0022-3042","type":"print"},{"value":"1471-4159","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6,23]]},"assertion":[{"value":"2022-12-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-05-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-06-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}