{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T05:02:01Z","timestamp":1789189321669,"version":"build-2803163510"},"reference-count":55,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,2,12]],"date-time":"2024-02-12T00:00:00Z","timestamp":1707696000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","award":["201409003900"],"award-info":[{"award-number":["201409003900"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","award":["18ZR1438500"],"award-info":[{"award-number":["18ZR1438500"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    In this study, we investigated the potential involvement of TNFSF9 in reperfusion injury associated with ferroptosis in acute ischaemic stroke patients, mouse models and BV2 microglia. We first examined TNFSF9 changes in peripheral blood from stroke patients with successful reperfusion, and constructed oxygen\u2013glucose deprivation\u2013reperfusion (OGD\u2010R) on BV2 microglia, oxygen\u2013glucose deprivation for 6\u2009h followed by reoxygenation and re\u2010glucose for 24\u2009h, and appropriate over\u2010expression or knockdown of TNFSF9 manipulation on BV2 cells and found that in the case of BV2 cells encountering OGD\u2010R over\u2010expression of TNFSF9 resulted in increased BV2 apoptosis. Still, the knockdown of TNFSF9 ameliorated apoptosis and ferroptosis. In an in\u00a0vivo experiment, we constructed TNFSF9 over\u2010expression or knockout mice by intracerebral injection of TNFSF9\u2010OE or sh\u2010TNFSF9 adenovirus. We performed the middle cerebral artery occlusion (MCAO) model on day four, 24\u2009h after ligation of the proximal artery, for half an hour to recanalize. As luck would have it, over\u2010expression of TNFSF9 resulted in increased brain infarct volumes, neurological function scores and abnormalities in TNFSF9\u2010related TRAF1 and ferroptosis\u2010related pathways, but knockdown of TNFSF9 improved brain infarcts in mice as well as reversing TNFSF9\u2010related signalling pathways. In conclusion, our data provide the first evidence that TNFSF9 triggers microglia activation by activating the ferroptosis signalling pathway following ischaemic stroke, leading to brain injury and neurological deficits.\n                    <jats:boxed-text content-type=\"graphic\" position=\"anchor\">\n                      <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc16058-fig-0011-m.png\">\n                        <jats:alt-text>image<\/jats:alt-text>\n                      <\/jats:graphic>\n                    <\/jats:boxed-text>\n                  <\/jats:p>","DOI":"10.1111\/jnc.16058","type":"journal-article","created":{"date-parts":[[2024,2,12]],"date-time":"2024-02-12T04:49:09Z","timestamp":1707713349000},"page":"1030-1044","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Ferroptosis mediated by\n                    <scp>TNFSF9<\/scp>\n                    interferes in acute ischaemic stroke reperfusion injury with the progression of acute ischaemic stroke"],"prefix":"10.1111","volume":"168","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9332-3786","authenticated-orcid":false,"given":"Zifu","family":"Li","sequence":"first","affiliation":[{"name":"Neurovascular center Changhai Hospital  Shanghai P. 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