{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T04:52:43Z","timestamp":1789102363556,"version":"build-2803163510"},"reference-count":46,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,9,25]],"date-time":"2024-09-25T00:00:00Z","timestamp":1727222400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Reactive astrocyte activation in the context of cerebral ischemia\/reperfusion (I\/R) injury gives rise to two distinct subtypes: the neurotoxic A1 type and the neuroprotective A2 type. DJ\u20101 (Parkinson disease protein 7, PARK7), originally identified as a Parkinson's disease\u2010associated protein, is a multifunctional anti\u2010oxidative stress protein with molecular chaperone and signaling functions. SHP\u20101 (Src homology 2 domain\u2010containing phosphatase\u20101) is a protein tyrosine phosphatase closely associated with cellular signal transduction. miR\u2010155 is a microRNA that participates in cellular functions by regulating gene expression. Recent studies have uncovered the relationship between DJ\u20101 and astrocyte\u2010mediated neuroprotection, which may be related to its antioxidant properties and regulation of signaling molecules such as SHP\u20101. Furthermore, miR\u2010155 may exert its effects by influencing SHP\u20101, providing a potential perspective for understanding the molecular mechanisms of stroke. A middle cerebral artery occlusion\/reperfusion (MCAO\/R) model and an oxygen\u2013glucose deprivation\/reperfusion (OGD\/R) model were established to simulate focal cerebral I\/R injury in\u00a0vivo and in\u00a0vitro, respectively. The in\u00a0vivo interaction between DJ\u20101 and SHP\u20101 has been experimentally validated through immunoprecipitation. Overexpression of DJ\u20101 attenuates I\/R injury and suppresses miR\u2010155 expression. In addition, inhibition of miR\u2010155 upregulates SHP\u20101 expression and modulates astrocyte activation phenotype. These findings suggest that DJ\u20101 mediates astrocyte activation via the miR\u2010155\/SHP\u20101 pathway, playing a pivotal role in the pathogenesis of cerebral ischemia\u2013reperfusion injury. Our results provide a potential way for exploring the pathogenesis of ischemic stroke and present promising targets for pharmacological intervention.\n                    <jats:boxed-text content-type=\"graphic\" position=\"anchor\">\n                      <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc16230-fig-0007-m.png\">\n                        <jats:alt-text>image<\/jats:alt-text>\n                      <\/jats:graphic>\n                    <\/jats:boxed-text>\n                  <\/jats:p>","DOI":"10.1111\/jnc.16230","type":"journal-article","created":{"date-parts":[[2024,9,26]],"date-time":"2024-09-26T00:59:35Z","timestamp":1727312375000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["<scp>DJ<\/scp>\n                    \u20101 regulates astrocyte activation through\n                    <scp>miR<\/scp>\n                    \u2010155\/\n                    <scp>SHP<\/scp>\n                    \u20101 signaling in cerebral ischemia\/reperfusion injury"],"prefix":"10.1111","volume":"169","author":[{"given":"Ying","family":"Xue","sequence":"first","affiliation":[{"name":"Department of Pathology Chongqing Medical University  Chongqing China"},{"name":"Department of Dermatology Chengdu Qingbaijiang District People's Hospital  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pathology Chongqing Medical University  Chongqing China"},{"name":"Molecular Medicine Diagnostic and Testing Center Chongqing Medical University  Chongqing China"},{"name":"Department of Pathology The First Affiliated Hospital of Chongqing Medical University  Chongqing 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