{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T22:22:23Z","timestamp":1789424543576,"version":"build-2803163510"},"reference-count":36,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,10,18]],"date-time":"2024-10-18T00:00:00Z","timestamp":1729209600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000288","name":"Royal Society","doi-asserted-by":"publisher","award":["NA 150482"],"award-info":[{"award-number":["NA 150482"]}],"id":[{"id":"10.13039\/501100000288","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004950","name":"Aston University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004950","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Puerarin, a natural isoflavone, is commonly used as a Chinese herbal medicine for the treatment of various cardiovascular and neurological disorders. It has been found to be neuroprotective via TrK\u2010PI3K\/Akt pathway, which is associated with anti\u2010inflammatory and antioxidant effects. Myelin damage in diseases such as multiple sclerosis (MS) and ischemia induces activation of endogenous oligodendrocyte progenitor cells (OPC) and subsequent remyelination by newly formed oligodendrocytes. It has been shown that human\u2010induced pluripotent stem cells (hiPSC)\u2010derived OPCs promote remyelination when transplanted to the brains of disease models. Here, we ask whether and how puerarin is beneficial to the generation of hiPSC\u2010derived OPCs and oligodendrocytes, and to the endogenous remyelination in mouse demyelination model. Our results show that puerarin increases the proportion of O4+ pre\u2010oligodendrocytes differentiated from iPSC\u2010derived neural stem cells. In\u00a0vitro, puerarin increases proliferation of rat OPCs and enhances mitochondrial activity. Treatment of puerarin at progenitor stage increases the yielding of differentiated oligodendrocytes. In rat organotypic brain slice culture, puerarin promotes both myelination and remyelination. In\u00a0vivo, puerarin increases oligodendrocyte repopulation during remyelination in mouse spinal cord following lysolethicin\u2010induced demyelination. Our findings suggest that puerarin promotes oligodendrocyte lineage progression and myelin repair, with a potential to be developed into therapeutic agent for neurological diseases associated with myelin damage.\n                    <jats:boxed-text content-type=\"graphic\" position=\"anchor\">\n                      <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc16245-fig-0007-m.png\">\n                        <jats:alt-text>image<\/jats:alt-text>\n                      <\/jats:graphic>\n                    <\/jats:boxed-text>\n                  <\/jats:p>","DOI":"10.1111\/jnc.16245","type":"journal-article","created":{"date-parts":[[2024,10,18]],"date-time":"2024-10-18T22:06:07Z","timestamp":1729289167000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The isoflavone puerarin promotes generation of human iPSC\u2010derived pre\u2010oligodendrocytes and enhances endogenous remyelination in rodent models"],"prefix":"10.1111","volume":"169","author":[{"given":"Hao","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine The Second Affiliated Hospital of Xi'an Jiaotong University  Xi'an Shaanxi China"},{"name":"School of Nursing, Health Science Center Xi'an Jiaotong University  Xi'an Shaanxi China"},{"name":"Department of Clinical Neurosciences and Wellcome Trust\u2010Medical Research Council Cambridge Stem Cell Institute University of Cambridge  Cambridge UK"},{"name":"Molecular Pharmacology Laboratory Institute of Molecular Medicine, Peking University  Beijing China"},{"name":"ALLIFE Medicine Science and Technology Co. Ltd. Building No. 13, VPark, Yizhuang Economic and Technological Development Zone  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiyuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Clinical Neurosciences and Wellcome Trust\u2010Medical Research Council Cambridge Stem Cell Institute University of Cambridge  Cambridge UK"},{"name":"Molecular Pharmacology Laboratory Institute of Molecular Medicine, Peking University  Beijing China"},{"name":"ALLIFE Medicine Science and Technology Co. Ltd. Building No. 13, VPark, Yizhuang Economic and Technological Development Zone  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nona","family":"Pop","sequence":"additional","affiliation":[{"name":"Aston Medical School, College of Health and Life Sciences, Aston University  Birmingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joe","family":"Hall","sequence":"additional","affiliation":[{"name":"Aston Medical School, College of Health and Life Sciences, Aston University  Birmingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ibrahim","family":"Shazlee","sequence":"additional","affiliation":[{"name":"Aston Medical School, College of Health and Life Sciences, Aston University  Birmingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moritz","family":"Wagner\u2010Tsukamoto","sequence":"additional","affiliation":[{"name":"Aston Medical School, College of Health and Life Sciences, Aston University  Birmingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiguo","family":"Chen","sequence":"additional","affiliation":[{"name":"Cell Therapy Center Beijing Institute of Geriatrics, Xuanwu Hospital Capital Medical University, National Clinical Research Center for Geriatric Diseases, and Key Laboratory of Neurodegenerative Diseases, Ministry of Education  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuchun","family":"Gu","sequence":"additional","affiliation":[{"name":"Molecular Pharmacology Laboratory Institute of Molecular Medicine, Peking University  Beijing China"},{"name":"ALLIFE Medicine Science and Technology Co. Ltd. Building No. 13, VPark, Yizhuang Economic and Technological Development Zone  Beijing China"},{"name":"Aston Medical School, College of Health and Life Sciences, Aston University  Birmingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Clinical Neurosciences and Wellcome Trust\u2010Medical Research Council Cambridge Stem Cell Institute University of Cambridge  Cambridge UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8628-8954","authenticated-orcid":false,"given":"Dan","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Clinical Neurosciences and Wellcome Trust\u2010Medical Research Council Cambridge Stem Cell Institute University of Cambridge  Cambridge UK"},{"name":"Aston Medical School, College of Health and Life Sciences, Aston University  Birmingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,10,18]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1103709"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401\u2010013\u20101112\u2010y"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.23733"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41387\u2010017\u20100009\u20106"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.149"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2015.08.013"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/1873\u20103468.13608"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201505119"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2015.075"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn.2017.136"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.714169"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2023.175695"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2023.114581"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms241612912"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.24466"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2021.153915"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2019.08.015"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1177\/1352458519884006"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2022.972144"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2020.05.006"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.22\u201007\u201002451.2002"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018\u2010021\u201003802\u20100"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.11.019"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.5966\/sctm.2016\u20100024"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cplett.2007.12.072"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.1022053"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2012.12.002"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2004.01.001"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep35475"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.14336\/AD.2021.1205"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2008.04.007"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3655-14.2015"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3655\u201014.2015"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2022.102205"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.21736"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.21052"}],"container-title":["Journal of Neurochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jnc.16245","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T19:51:19Z","timestamp":1741722679000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jnc.16245"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,18]]},"references-count":36,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.1111\/jnc.16245"],"URL":"https:\/\/doi.org\/10.1111\/jnc.16245","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1111\/JNC.16245\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JNC.16245\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JNC.16245\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JNC.16245\/v3\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JNC.16245\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JNC.16245\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JNC.16245\/v3\/response1","asserted-by":"object"}]},"ISSN":["0022-3042","1471-4159"],"issn-type":[{"value":"0022-3042","type":"print"},{"value":"1471-4159","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,18]]},"assertion":[{"value":"2024-03-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-09-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e16245"}}