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Proper functioning of the central nervous system (CNS) depends on blood\u2013brain barrier (BBB) development, which is formed by interactions of vascular endothelial cells, pericytes, astrocytes, and basal lamina. Gestational exposure to ethanol has been demonstrated to impair CNS development; however, little is known about ethanol modulation of blood circulating factors and impacts on human developing BBB. Here we investigated the prevalence of alcohol consumption during pregnancy and found that 27% of pregnant women reported alcohol consumption, mainly in the first trimester. Control and alcohol\u2010exposed newborns showed no differences in weight, length, and appearance, pulse, grimace, activity, respiration (APGAR) score at birth. In\u00a0vitro, we cultivated human brain microcapillary endothelial cells (HBMEC) and treated with umbilical cord blood serum (UCBS) from control (S\u2010Control) newborns or ethanol\u2010exposed ones (S\u2010Ethanol). S\u2010Ethanol treatment induced 68% and 38% decreases in protein levels of ZO\u20101 (tight junction) and GLUT\u20101 (glucose transporter type\u20101), respectively, increased endothelial monolayer permeability, migratory potential impairment, and changes in angiogenesis\u2010related secreted proteins profile, compared to S\u2010Control treatments. UCBS proteomics revealed a total of 392 proteins, 10 exclusively found in S\u2010Ethanol, mostly related to innate and adaptive immunity and tissue injury response. These results suggest that gestational exposure to ethanol contributes to blood altered protein profiles triggering BBB endothelial.\n                    <jats:boxed-text content-type=\"graphic\" position=\"anchor\">\n                      <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc70015-fig-0005-m.png\">\n                        <jats:alt-text>image<\/jats:alt-text>\n                      <\/jats:graphic>\n                    <\/jats:boxed-text>\n                  <\/jats:p>","DOI":"10.1111\/jnc.70015","type":"journal-article","created":{"date-parts":[[2025,2,10]],"date-time":"2025-02-10T22:25:13Z","timestamp":1739226313000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Prenatal Alcohol Consumption Alters Protein Fingerprint in Umbilical Cord Blood Serum and Induces Brain Microvascular Endothelial Cell Dysfunction"],"prefix":"10.1111","volume":"169","author":[{"given":"Paula Silva Lacerda","family":"Almeida","sequence":"first","affiliation":[{"name":"Instituto de Ci\u00eancias Biom\u00e9dicas, Universidade Federal do Rio de Janeiro  Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dayana","family":"Ara\u00fajo","sequence":"additional","affiliation":[{"name":"N\u00facleo Perinatal Hospital Universit\u00e1rio Pedro Ernesto, Universidade do Estado do Rio de Janeiro  Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3126-4984","authenticated-orcid":false,"given":"Juliana Minardi","family":"Nascimento","sequence":"additional","affiliation":[{"name":"Instituto de Biof\u00edsica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro  Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex 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