{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T18:39:40Z","timestamp":1792003180612,"version":"4.4.0"},"reference-count":86,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,5,28]],"date-time":"2025-05-28T00:00:00Z","timestamp":1748390400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Autism spectrum disorder (ASD) is a neurodevelopmental psychiatric condition linked to glutamatergic neurotransmission disruption. Although endogenous D\u2010serine and D\u2010aspartate modulate glutamatergic N\u2010methyl D\u2010aspartate receptor (NMDAR) activity, their involvement in ASD remains elusive. We measured the levels of D\u2010aspartate, D\u2010serine, and other key neuroactive amino acids, and their direct precursors in brain regions, plasma, and feces of environmental ASD rat models prenatally exposed to lipopolysaccharide or valproate, both during adolescence and early adulthood, as well as in a genetic ASD model, the\n                    <jats:italic>\n                      Fmr1\u2010\n                      <jats:sup>\u0394<\/jats:sup>\n                      exon8\n                    <\/jats:italic>\n                    rat. No significant changes were found in plasma and feces. Conversely, we observed a prominent accumulation of D\u2010aspartate in several brain regions of lipopolysaccharide\u2010 and valproate\u2010exposed rats, selectively during adolescence, while D\u2010serine level variations were more limited. No significant amino acid changes were observed in the\n                    <jats:italic>\n                      Fmr1\u2010\n                      <jats:sup>\u0394<\/jats:sup>\n                      exon8\n                    <\/jats:italic>\n                    rat brain. We also assayed the activity of the main enzymes involved in cerebral D\u2010serine and D\u2010aspartate metabolism, suggesting that their regulation extends beyond their metabolic enzymes. These findings highlight that prenatal environmental stressors disrupt D\u2010amino acid levels selectively in ASD rat brains, emphasizing the role of early NMDAR dysfunction in ASD\u2010related phenotypes.\n                    <jats:boxed-text content-type=\"graphic\" position=\"anchor\">\n                      <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc70095-fig-0008-m.png\">\n                        <jats:alt-text>image<\/jats:alt-text>\n                      <\/jats:graphic>\n                    <\/jats:boxed-text>\n                  <\/jats:p>","DOI":"10.1111\/jnc.70095","type":"journal-article","created":{"date-parts":[[2025,5,29]],"date-time":"2025-05-29T02:56:31Z","timestamp":1748487391000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Prenatal Exposure to Lipopolysaccharide or Valproate Leads to Abnormal Accumulation of the NMDA Receptor Agonist D\u2010Aspartate in the Adolescent Rat Brain"],"prefix":"10.1111","volume":"169","author":[{"given":"Anna","family":"Di Maio","sequence":"first","affiliation":[{"name":"CEINGE Biotecnologie Avanzate \u201cFranco Salvatore\u201d  Naples Italy"},{"name":"Department of Environmental, Biological and Pharmaceutical Sciences and Technologies Universit\u00e0 degli Studi della Campania \u201cLuigi Vanvitelli\u201d  Caserta Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isar","family":"Yahyavi","sequence":"additional","affiliation":[{"name":"CEINGE Biotecnologie Avanzate \u201cFranco Salvatore\u201d  Naples Italy"},{"name":"Department of Environmental, Biological and Pharmaceutical Sciences and Technologies Universit\u00e0 degli Studi della Campania \u201cLuigi Vanvitelli\u201d  Caserta Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valeria","family":"Buzzelli","sequence":"additional","affiliation":[{"name":"Department of Science Roma Tre University  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zoraide","family":"Motta","sequence":"additional","affiliation":[{"name":"\u201cThe Protein Factory 2.0\u201d, Dipartimento di Biotecnologie e Scienze della Vita Universit\u00e0 degli Studi dell'insubria  Varese Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabrizio","family":"Ascone","sequence":"additional","affiliation":[{"name":"Department of Science Roma Tre University  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorenza","family":"Putignani","sequence":"additional","affiliation":[{"name":"Unit of Microbiomics and Unit of Research of Microbiome Bambino Ges\u00f9 Children's Hospital, IRCCS  Rome Italy"},{"name":"Department of Life Science, Health, and Health Professions, Link Campus University  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"Usiello","sequence":"additional","affiliation":[{"name":"CEINGE Biotecnologie Avanzate \u201cFranco Salvatore\u201d  Naples Italy"},{"name":"Department of Environmental, Biological and Pharmaceutical Sciences and Technologies Universit\u00e0 degli Studi della Campania \u201cLuigi Vanvitelli\u201d  Caserta Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Loredano","family":"Pollegioni","sequence":"additional","affiliation":[{"name":"\u201cThe Protein Factory 2.0\u201d, Dipartimento di Biotecnologie e Scienze della Vita Universit\u00e0 degli Studi dell'insubria  Varese Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3922-6045","authenticated-orcid":false,"given":"Viviana","family":"Trezza","sequence":"additional","affiliation":[{"name":"Department of Science Roma Tre University  Rome Italy"},{"name":"Neuroendocrinology, Metabolism and Neuropharmacology Unit IRCCS Fondazione Santa Lucia  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3636-7395","authenticated-orcid":false,"given":"Francesco","family":"Errico","sequence":"additional","affiliation":[{"name":"CEINGE Biotecnologie Avanzate \u201cFranco Salvatore\u201d  Naples Italy"},{"name":"Dipartimento di Agraria Universit\u00e0 degli Studi di Napoli \u201cFederico II\u201d  Portici Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,5,28]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1006\/faat.1997.2318"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbb.2010.12.026"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2018.12.002"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp-2012-304270"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2014.11.008"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.429"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24043920"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2013.2270"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1176\/appi.ajp.2020.20101481"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41537-022-00231-1"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-020-02839-y"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2020.140471"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpsychires.2013.06.013"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/tp.2014.59"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1618-08.2008"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2011.08354.x"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep37261"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopsych.2024.11.009"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpsyt.2024.1408175"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2300817120"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.0c00622"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2023.1135559"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2015.04.022"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1993.tb03575.x"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pep.2008.09.003"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuint.2013.02.015"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11910-018-0887-6"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2024.106413"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/jb\/mvw043"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00702-014-1174-6"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.15488"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2015.00017"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.200900250"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2017.12.009"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114517000678"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.4103\/0976-500X.72351"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.23089"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/cercor\/bhy120"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2011.01.030"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-023-03438-0"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41398-022-02088-5"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12916-024-03617-3"},{"issue":"12","key":"e_1_2_10_44_1","first-page":"713","article-title":"Diagnosis and Management of Obstetrical Antiphospholipid Syndrome: Where Do We Stand?","volume":"123","author":"Marchetti T.","year":"2013","journal-title":"Polskie Archiwum Medycyny Wewn\u0119trznej"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-36244-z"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.14435"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00213-019-05357-2"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201901703R"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2006.03.045"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23073861"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2023.109479"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1111\/jnc.13204"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-020-03574-z"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpsyt.2022.889636"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2017.04.017"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2020.140537"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41537-017-0015-7"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms251910469"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2015.05.007"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-010-0307-9"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2018.00107"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2020.140472"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3881-15.2016"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2018.00112"},{"key":"e_1_2_10_65_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41537-024-00537-2"},{"key":"e_1_2_10_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pnpbp.2024.111234"},{"key":"e_1_2_10_67_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41583-021-00432-0"},{"key":"e_1_2_10_68_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2017.00102"},{"key":"e_1_2_10_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(98)00599-X"},{"key":"e_1_2_10_70_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2016.125"},{"key":"e_1_2_10_71_1","doi-asserted-by":"publisher","DOI":"10.1080\/1028415X.2020.1819107"},{"key":"e_1_2_10_72_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2019.00479"},{"key":"e_1_2_10_73_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41386-022-01454-7"},{"key":"e_1_2_10_74_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2023.106226"},{"key":"e_1_2_10_75_1","doi-asserted-by":"publisher","DOI":"10.1038\/tp.2016.182"},{"key":"e_1_2_10_76_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2023.09.001"},{"key":"e_1_2_10_77_1","doi-asserted-by":"publisher","DOI":"10.1038\/tp.2014.69"},{"key":"e_1_2_10_78_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-022-03140-w"},{"key":"e_1_2_10_79_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2018.12.024"},{"key":"e_1_2_10_80_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOP.0b013e32835c2b70"},{"key":"e_1_2_10_81_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(91)90203-S"},{"key":"e_1_2_10_82_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.871086"},{"key":"e_1_2_10_83_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-neuro-071714-033821"},{"key":"e_1_2_10_84_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.23.13409"},{"key":"e_1_2_10_85_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(00)00321-3"},{"key":"e_1_2_10_86_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST20130220"},{"key":"e_1_2_10_87_1","doi-asserted-by":"publisher","DOI":"10.2174\/1570159X22666231003121513"}],"container-title":["Journal of Neurochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jnc.70095","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,19]],"date-time":"2025-07-19T15:22:10Z","timestamp":1752938530000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jnc.70095"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,28]]},"references-count":86,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2025,6]]}},"alternative-id":["10.1111\/jnc.70095"],"URL":"https:\/\/doi.org\/10.1111\/jnc.70095","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1111\/JNC.70095\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JNC.70095\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JNC.70095\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JNC.70095\/v3\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JNC.70095\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JNC.70095\/v3\/response1","asserted-by":"object"}]},"ISSN":["0022-3042","1471-4159"],"issn-type":[{"value":"0022-3042","type":"print"},{"value":"1471-4159","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5,28]]},"assertion":[{"value":"2025-02-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-05-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-05-28","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70095"}}