{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T12:15:42Z","timestamp":1791980142173,"version":"4.4.0"},"reference-count":34,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T00:00:00Z","timestamp":1751414400000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["U01 NS131740"],"award-info":[{"award-number":["U01 NS131740"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["U01 NS141777"],"award-info":[{"award-number":["U01 NS141777"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000025","name":"National Institute of Mental Health","doi-asserted-by":"publisher","award":["R01 MH108509"],"award-info":[{"award-number":["R01 MH108509"]}],"id":[{"id":"10.13039\/100000025","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000005","name":"U.S. Department of Defense","doi-asserted-by":"publisher","award":["HT94252320064"],"award-info":[{"award-number":["HT94252320064"]}],"id":[{"id":"10.13039\/100000005","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Phosphorylated tau (p\u2010tau) 217 is a promising blood biomarker for Alzheimer's disease (AD). However, most p\u2010tau217 assays have been validated solely in ethylenediaminetetraacetic acid (EDTA) plasma, leaving the clinical applicability of serum p\u2010tau217 largely unexplored despite serum being a preferred matrix in many clinical laboratories. To address this gap, we compared p\u2010tau217 concentrations and classification accuracies in matched plasma and serum samples in\u00a0four research\u2010use\u2010only assays. Paired plasma and serum samples were processed from the same venipuncture collection and assessed with each of the four p\u2010tau217 assays following manufacturer\u2010recommended procedures in two research cohorts from the University of Pittsburgh Azheimer's Disease Research Center (Pitt\u2010ADRC;\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200950) and the Human Connectome Project (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200934). The four assays evaluated included three from commercial sources\u2014Lumipulse (recently gained FDA approval), ALZpath, and NULISA\u2014and another from the University of Pittsburgh (Pittsburgh plasma p\u2010tau217). Plasma and serum p\u2010tau217 levels varied across assays; the ALZpath, Pittsburgh, and NULISA methods showed significantly lower p\u2010tau217 levels in serum compared with plasma (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.0001) for both cohorts, while Lumipulse showed higher plasma levels in the Pitt\u2010ADRC cohort but equivalent plasma and serum levels in the HCP cohort. Yet, strong correlations (rho &gt;\u20090.8) were observed between plasma and serum p\u2010tau217 pairs for all methods. Both plasma and serum p\u2010tau217 demonstrated strong classification accuracies to differentiate clinical AD from normal controls, with high AUC (up to 0.963) for all methods. The exception was the Pittsburgh assay, where plasma p\u2010tau217 had significantly superior AUC to serum p\u2010tau217 (plasma: 0.912, serum: 0.844). The rest of the assays had equivalent accuracies in both matrices. Serum p\u2010tau217 performs equivalently as plasma p\u2010tau217 for most assessed assays. Serum can be used as a substitute for plasma in the use of most p\u2010tau217 assays to\u00a0evaluate A\u03b2 pathology in AD for both research and clinical purposes.\n                    <jats:boxed-text content-type=\"graphic\" position=\"anchor\">\n                      <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc70114-fig-0006-m.png\">\n                        <jats:alt-text>image<\/jats:alt-text>\n                      <\/jats:graphic>\n                    <\/jats:boxed-text>\n                  <\/jats:p>","DOI":"10.1111\/jnc.70114","type":"journal-article","created":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T05:15:23Z","timestamp":1751433323000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Equivalence of Plasma and Serum for Clinical Measurement of p\u2010tau217: Comparative Analyses of Four Blood\u2010Based Assays"],"prefix":"10.1111","volume":"169","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-4579-4057","authenticated-orcid":false,"given":"Yijun","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Psychiatry, School of Medicine University of Pittsburgh  Pittsburgh Pennsylvania USA"},{"name":"Department of Chemistry University of Pittsburgh  Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ally L.","family":"Albert","sequence":"additional","affiliation":[{"name":"Department of Psychiatry, School of Medicine University of Pittsburgh  Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anuradha","family":"Sehrawat","sequence":"additional","affiliation":[{"name":"Department of Psychiatry, School of Medicine University of Pittsburgh  Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marissa F.","family":"Farinas","sequence":"additional","affiliation":[{"name":"Department of Psychiatry, School of Medicine University of Pittsburgh  Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oscar","family":"L. Lopez","sequence":"additional","affiliation":[{"name":"Department of Psychiatry, School of Medicine University of Pittsburgh  Pittsburgh Pennsylvania USA"},{"name":"Department of Neurology, School of Medicine University of Pittsburgh  Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuemei","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Psychiatry, School of Medicine University of Pittsburgh  Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ann D.","family":"Cohen","sequence":"additional","affiliation":[{"name":"Department of Psychiatry, School of Medicine University of Pittsburgh  Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas K.","family":"Karikari","sequence":"additional","affiliation":[{"name":"Department of Psychiatry, School of Medicine University of Pittsburgh  Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,2]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/prca.201100061"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/dad2.12168"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/YCO.0000000000000851"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2015.03.002"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnagi.2021.669490"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jalz.2016.02.002"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaneurol.2024.3770"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/alz.13393"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jagp.2024.09.015"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jagp.2024.09.015"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/alz.13525"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13024-023-00605-8"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/alz.13859"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13195-022-01011-w"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41582-022-00665-2"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0249797"},{"key":"e_1_2_12_18_1","first-page":"434","article-title":"Population\u2010Based Blood Screening for Preclinical Alzheimer's Disease in a British Birth Cohort at Age 70","volume":"144","author":"Keshavan A.","year":"2021","journal-title":"Brain: A Journal of Neurology"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.55.12.1854"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.55.12.1863"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.15252\/emmm.202114022"},{"key":"e_1_2_12_22_1","first-page":"1797","article-title":"Plasma p\u2010tau231 and p\u2010tau217 as State Markers of Amyloid\u2010\u03b2 Pathology in Preclinical Alzheimer's Disease","volume":"28","author":"Mil\u00e0\u2010Alom\u00e0 M.","year":"2022","journal-title":"Nature 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