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Therefore, reliable biomarkers are needed to help identify subjects at risk and allow for early intervention, as well as for better monitoring of disease progression and response to therapy. \u03b1\u2010Synuclein (\u03b1\u2010syn) has emerged as a potential biomarker for PD, along with other biomarkers such as cytokines, amyloid \u03b242, tau protein, and neurofilament light chain. Despite the abundance of biomarker discovery research, comparative studies and biomarker validation have been difficult due to the lack of large longitudinal studies and the corresponding bioanalytical challenges, which limit accurate biomarker measurements and identification. Key bioanalytical challenges in biomarker identification include the need for very sensitive assays with low limits of detection and quantitation, low sample volumes, potential matrix effects, significant amounts of pre\u2010analytical sample processing, and the need to avoid interpretive bias. 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