{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T23:49:35Z","timestamp":1789775375029,"version":"4.0.1"},"reference-count":52,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,7,19]],"date-time":"2025-07-19T00:00:00Z","timestamp":1752883200000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["2020.06582.BD"],"award-info":[{"award-number":["2020.06582.BD"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["2022.01066.PTDC"],"award-info":[{"award-number":["2022.01066.PTDC"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["FCT\/UIDP&B\/04950\/2025"],"award-info":[{"award-number":["FCT\/UIDP&B\/04950\/2025"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The maternal diet is a key determinant of offspring neurodevelopment, influencing gut microbiota composition and the gut\u2010brain axis. These interactions are critical in shaping sex\u2010specific vulnerabilities to neuropsychiatric disorders. Tryptophan, an essential amino acid and precursor to serotonin, plays a pivotal role in neurodevelopment and immune function. However, the impact of maternal tryptophan supplementation on healthy offspring's neurochemical and behavioral outcomes remains unclear. This study investigated the effects of a tryptophan\u2010enriched maternal diet on offspring gut microbiota, metabolism, neurochemical pathways, and behavior. We analyzed microbial composition, metabolic activity, brain, and plasma levels of tryptophan and serotonin, and behavioral outcomes in male and female mice offspring. Maternal tryptophan supplementation led to sex\u2010specific changes. Female offspring exhibited disrupted microbiota diversity, reduced brain tryptophan and serotonin levels, and heightened anxiety\u2010like behaviors. In contrast, male offspring displayed increased metabolic activity, elevated brain tryptophan levels, and repetitive behaviors. These findings suggest that maternal tryptophan supplementation influences offspring neurodevelopment and behavior by modulating gut\u2010brain axis components in a sex\u2010dependent manner. This work underscores the importance of further research into prenatal dietary interventions targeting gut microbiota and neurochemical pathways to support healthy neurodevelopment and potentially inform sex\u2010specific preventive strategies. These findings contribute to a growing understanding of how maternal nutrition influences offspring neurodevelopment and may guide the development of preventive strategies to improve long\u2010term mental health outcomes..\n                    <jats:boxed-text content-type=\"graphic\" position=\"anchor\">\n                      <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc70161-fig-0007-m.png\">\n                        <jats:alt-text>image<\/jats:alt-text>\n                      <\/jats:graphic>\n                    <\/jats:boxed-text>\n                  <\/jats:p>","DOI":"10.1111\/jnc.70161","type":"journal-article","created":{"date-parts":[[2025,7,19]],"date-time":"2025-07-19T08:19:48Z","timestamp":1752913188000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Maternal Tryptophan Supplementation Alters Offspring Gut\u2010Brain Axis and Behavior in a Sex\u2010Specific Manner"],"prefix":"10.1111","volume":"169","author":[{"given":"Maria Carolina","family":"Fortunato","sequence":"first","affiliation":[{"name":"Coimbra Institute for Biomedical Imaging and Translational Research (CIBIT) University of Coimbra  Coimbra Portugal"},{"name":"Faculty of Pharmacy, Laboratory of Pharmacology and Pharmaceutical Care University of Coimbra  Coimbra Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o","family":"Neves","sequence":"additional","affiliation":[{"name":"Coimbra Institute for Biomedical Imaging and Translational Research (CIBIT) University of Coimbra  Coimbra Portugal"},{"name":"Institute of Nuclear Sciences Applied to Health (ICNAS) University of Coimbra  Coimbra Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana Lapo","family":"Pais","sequence":"additional","affiliation":[{"name":"Coimbra Institute for Biomedical Imaging and Translational Research (CIBIT) University of Coimbra  Coimbra Portugal"},{"name":"Institute of Nuclear Sciences Applied to Health (ICNAS) University of Coimbra  Coimbra Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla","family":"Fonseca","sequence":"additional","affiliation":[{"name":"Coimbra Institute for Biomedical Imaging and Translational Research (CIBIT) University of Coimbra  Coimbra Portugal"},{"name":"Faculty of Pharmacy, Laboratory of Pharmacology and Pharmaceutical Care University of Coimbra  Coimbra Portugal"},{"name":"Department of Pharmacology, Toxicology and Therapeutic Chemistry, Faculty of Pharmacy and Food Science Universitat de Barcelona  Barcelona Spain"},{"name":"Institute of Neurosciences Universitat de Barcelona  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Silva","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Laboratory of Pharmacology and Pharmaceutical Care University of Coimbra  Coimbra Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o","family":"Martins","sequence":"additional","affiliation":[{"name":"Coimbra Institute for Biomedical Imaging and Translational Research (CIBIT) University of Coimbra  Coimbra Portugal"},{"name":"Institute of Nuclear Sciences Applied to Health (ICNAS) University of Coimbra  Coimbra Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4364-6373","authenticated-orcid":false,"given":"Miguel","family":"Castelo\u2010Branco","sequence":"additional","affiliation":[{"name":"Coimbra Institute for Biomedical Imaging and Translational Research (CIBIT) University of Coimbra  Coimbra Portugal"},{"name":"Institute of Nuclear Sciences Applied to Health (ICNAS) University of Coimbra  Coimbra Portugal"},{"name":"Faculty of Medicine, Institute of Physiology University of Coimbra  Coimbra Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Fortuna","sequence":"additional","affiliation":[{"name":"Coimbra Institute for Biomedical Imaging and Translational Research (CIBIT) University of Coimbra  Coimbra Portugal"},{"name":"Faculty of Pharmacy, Laboratory of Pharmacology and Pharmaceutical Care University of Coimbra  Coimbra Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joana","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"Coimbra Institute for Biomedical Imaging and Translational Research (CIBIT) University of Coimbra  Coimbra Portugal"},{"name":"Institute of Nuclear Sciences Applied to Health (ICNAS) University of Coimbra  Coimbra Portugal"},{"name":"Faculty of Medicine, Institute of Physiology University of Coimbra  Coimbra Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,19]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2018.05.003"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3791\/50978"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13213\u2010020\u201001608\u20102"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40168\u2010018\u20100470\u2010z"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587\u2010019\u20100209\u20109"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2011.12.009"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0175577"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.06.001"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2017.119"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopsych.2017.06.021"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu10030274"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41596\u2010019\u20100264\u20101"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/mp.2012.77"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/nxaa343"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2022.111461"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cgh.2018.08.067"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.euroneuro.2015.03.005"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010019\u201009382\u20109"},{"key":"e_1_2_10_20_1","unstructured":"European Medicines Agency.2025.\u201cICH M10 on Bioanalytical Method Validation\u2010Scientific Guideline | European Medicines Agency (EMA).\u201dhttps:\/\/www.ema.europa.eu\/en\/ich\u2010m10\u2010bioanalytical\u2010method\u2010validation\u2010scientific\u2010guideline."},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00726\u2010012\u20101330\u2010y"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/metabo12010071"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s41664\u2010024\u201000307\u20100"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/advances\/nmz127"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13229\u2010016\u20100109\u20105"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpobgyn.2013.08.017"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1039\/c8fo00444g"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1010529108"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2011.41"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yfrne.2019.100797"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/metabo11080482"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2020.11.003"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks808"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2023.02.037"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13041\u2010017\u20100292\u20100"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10803\u2010015\u20102646\u20108"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu13124510"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41387\u2010018\u20100027\u2010z"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.7759\/cureus.48763"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2014.07.027"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks1219"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2108581119"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/ppe.12414"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010018\u201005470\u20104"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22062973"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1462399406000068"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neubiorev.2022.104577"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13293\u2010023\u201000509\u20108"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974\u2010024\u201003030\u2010w"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1111\/mcn.12992"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010020\u20102745\u20103"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.44"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1097\/WCO.0b013e32835ee548"}],"container-title":["Journal of Neurochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jnc.70161","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,29]],"date-time":"2025-07-29T05:47:40Z","timestamp":1753768060000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jnc.70161"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7]]},"references-count":52,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2025,7]]}},"alternative-id":["10.1111\/jnc.70161"],"URL":"https:\/\/doi.org\/10.1111\/jnc.70161","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1111\/JNC.70161\/v1\/review4","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JNC.70161\/v3\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JNC.70161\/v1\/review3","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JNC.70161\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JNC.70161\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JNC.70161\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JNC.70161\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JNC.70161\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JNC.70161\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JNC.70161\/v3\/decision1","asserted-by":"object"}]},"ISSN":["0022-3042","1471-4159"],"issn-type":[{"value":"0022-3042","type":"print"},{"value":"1471-4159","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7]]},"assertion":[{"value":"2025-01-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-04","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70161"}}