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We obtained cells from subependymal giant cell astrocytoma (SEGA) and cortical tuber (CTuber) tissues of TSC patients to establish SEGA and CTuber cell strains. Focusing on these patient\u2010derived cell strains, we screened eight compounds from various drug classes for their effects on cell viability in\u00a0vitro. Five of these compounds demonstrated significant reductions in cell viability and effectively disrupted mTORC1 signaling and autophagy. Trifluoperazine, which exhibited no adverse effects on normal astrocytes, was selected for further study. Combination studies with rapamycin were conducted to evaluate their joint effects on cell viability and autophagy abnormalities, especially in SEGA\u2010derived cells from rapamycin\u2010resistant patients. Notably, trifluoperazine showed promising properties by maintaining its efficacy in combination with rapamycin and effectively reducing cell viability, even in rapamycin\u2010resistant SEGA\u2010derived cells. Trifluoperazine appears promising as a treatment for TSC. However, further research is crucial to confirm its therapeutic benefits and safety profile in TSC patients, representing a significant direction for future TSC management studies.<\/jats:p>\n                  <jats:p>\n                    <jats:boxed-text content-type=\"graphic\" position=\"anchor\">\n                      <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc70247-gra-0001-m.png\">\n                        <jats:alt-text>image<\/jats:alt-text>\n                      <\/jats:graphic>\n                    <\/jats:boxed-text>\n                  <\/jats:p>","DOI":"10.1111\/jnc.70247","type":"journal-article","created":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T05:17:13Z","timestamp":1759814233000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Trifluoperazine, an Antipsychotic Drug, Inhibits Viability of Cells Derived From\n                    <scp>SEGA<\/scp>\n                    and Cortical Tubers Cultured In\u00a0Vitro"],"prefix":"10.1111","volume":"169","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9521-0366","authenticated-orcid":false,"given":"Malgorzata","family":"Urbanska","sequence":"first","affiliation":[{"name":"Department of Neurology and Epileptology The Children's Memorial Health Institute  Warsaw Poland"},{"name":"Laboratory of Molecular and Cellular Neurobiology International Institute of Molecular and Cell Biology  Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Krzysztof","family":"Sadowski","sequence":"additional","affiliation":[{"name":"Department of Neurology and Epileptology The Children's Memorial Health Institute  Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aleksandra","family":"Stawikowska","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular and Cellular Neurobiology International Institute of Molecular and Cell Biology  Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ewa","family":"Liszewska","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular and Cellular Neurobiology International Institute of Molecular and Cell Biology  Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magdalena","family":"Mlostek","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular and Cellular Neurobiology International Institute of Molecular and Cell Biology  Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wieslawa","family":"Grajkowska","sequence":"additional","affiliation":[{"name":"Department of Pathology The Children's Memorial Health Institute  Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katarzyna","family":"Kotulska","sequence":"additional","affiliation":[{"name":"Department of Neurology and Epileptology The Children's Memorial Health Institute  Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,10,6]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1158\/2159\u20108290.CD\u201019\u20100292"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1920154117"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1528\u20101167.2009.02474.x"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.26551"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejpn.2018.05.006"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003\u2010020\u20100833\u20104"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.0c01618"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419\u2010018\u20101046\u20103"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(16)31419\u20102"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1176\/appi.neuropsych.13020035"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13073\u2010016\u20100347\u20103"},{"key":"e_1_2_10_13_1","first-page":"139","article-title":"Brain Lesions in Tuberous Sclerosis Complex. 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