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Understanding the regulatory functions of c\u2010Myc has been challenging due to its intricate and multifaceted roles in cellular processes. The\n                    <jats:italic>ATP13A2<\/jats:italic>\n                    (\n                    <jats:italic>PARK9<\/jats:italic>\n                    ) gene encodes the ATP13A2 protein, which has important roles in lysosomal functions and metal ion transport. The association of ATP13A2 with Kufor\u2010Rakeb Syndrome (KRS), as well as its role in Parkinson's disease, highlights its significance in maintaining cellular homeostasis. While our previous study indicated that c\u2010Myc might play a role in the regulation of the\n                    <jats:italic>ATP13A2<\/jats:italic>\n                    gene and its mutation linked to KRS, very little is known about the transcriptional regulation of the\n                    <jats:italic>ATP13A2<\/jats:italic>\n                    gene. In this study, we identified potential c\u2010Myc transcription factor binding sites on the\n                    <jats:italic>ATP13A2<\/jats:italic>\n                    promoter and showed in\u00a0vivo c\u2010Myc binding using ChIP assay. qPCR and luciferase analyses revealed that the\n                    <jats:italic>ATP13A2<\/jats:italic>\n                    transcription level was decreased upon 36\u2009h of c\u2010Myc overexpression. In contrast, western blot analysis revealed an increased ATP13A2 protein level under the same conditions. We further analyzed this discrepancy in a time\u2010dependent manner, and results indicated that after c\u2010Myc overexpression, ATP13A2 expression was markedly upregulated for the first 24\u2009h, but this impact gradually decreased, returning to baseline levels by 72\u2009h. Both HIF1\u03b1 and p53 exhibited transient upregulation followed by a time\u2010dependent decrease, suggesting that the initial increase in ATP13A2 may be regulated by c\u2010Myc\u2010driven HIF1\u03b1 stabilization, which was supported by the elevated ATP13A2 expression and HIF1\u03b1 stabilization by CoCl\n                    <jats:sub>2<\/jats:sub>\n                    treatment. Prussian blue analysis indicated corresponding changes in intracellular iron accumulation with the temporal alterations in ATP13A2 expression. Our findings indicate that c\u2010Myc indirectly causes an increased\n                    <jats:italic>ATP13A2<\/jats:italic>\n                    expression by increasing HIF1\u03b1 accumulation.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:boxed-text content-type=\"graphic\" position=\"anchor\">\n                      <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc70296-gra-0001-m.png\">\n                        <jats:alt-text>image<\/jats:alt-text>\n                      <\/jats:graphic>\n                    <\/jats:boxed-text>\n                  <\/jats:p>","DOI":"10.1111\/jnc.70296","type":"journal-article","created":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T05:21:26Z","timestamp":1764134486000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["C\u2010Myc Indirectly Controls\n                    <scp>ATP<\/scp>\n                    13\n                    <scp>A<\/scp>\n                    2 Levels via\n                    <scp>HIF<\/scp>\n                    \u20101\u03b1 Activation"],"prefix":"10.1111","volume":"169","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7593-0072","authenticated-orcid":false,"given":"Asl\u0131 Beril","family":"Tiryakiler","sequence":"first","affiliation":[{"name":"Molecular Biology\u2010Genetics and Biotechnology, Graduate School Istanbul Technical University  Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1872-941X","authenticated-orcid":false,"given":"Benan","family":"Temizci","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics Istanbul Technical University  Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5150-5349","authenticated-orcid":false,"given":"Arzu","family":"Karabay","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics Istanbul Technical University  Istanbul 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