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Treg\u2010derived extracellular vesicles (Treg\u2010EVs) therapy, which circumvents the safety risks associated with live cell therapies, exhibits the potential to modulate metabolic and immune functions, offering promise for healthy aging. Here, we isolated Tregs from young male C57BL\/6 mice and collected Treg\u2010EVs. In\u00a0vitro experiments demonstrated that Treg\u2010EVs significantly attenuated cellular senescence, reduced reactive oxygen species (ROS) accumulation, and enhanced mitochondrial respiration in HL\u20101 and HT22 senescent cell models. In\u00a0vivo experimental data revealed that young Treg\u2010EVs promoted mitochondrial biogenesis, facilitated vascular repair and regeneration, as well as attenuated inflammatory responses, and ultimately prolonged the survival of aged male C57BL\/6 mice. This study demonstrates the ability of Treg\u2010EVs therapy to reverse multiple aging\u2010related abnormal phenotypes, providing a promising strategy for treating aging and its associated diseases.<\/jats:p>\n                  <jats:p>\n                    <jats:boxed-text content-type=\"graphic\" position=\"anchor\">\n                      <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc70402-gra-0001-m.png\">\n                        <jats:alt-text>image<\/jats:alt-text>\n                      <\/jats:graphic>\n                    <\/jats:boxed-text>\n                  <\/jats:p>","DOI":"10.1111\/jnc.70402","type":"journal-article","created":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T05:10:43Z","timestamp":1773033043000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Young Regulatory 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