{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T07:59:52Z","timestamp":1790323192488,"version":"4.1.0"},"reference-count":56,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T00:00:00Z","timestamp":1780012800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T00:00:00Z","timestamp":1780012800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Ischemic stroke frequently results in sympathetic overactivity and blood pressure dysregulation, primarily due to impaired central autonomic regulation within the rostral ventrolateral medulla (RVLM) in the brainstem. However, the molecular mechanisms underlying this dysfunction remain insufficiently defined. In this study, we examined the regulatory interplay between the renin\u2013angiotensin system (RAS), autophagy, and iron metabolism in the RVLM in a transient middle cerebral artery occlusion model (MCAO) of ischemic stroke in Sprague\u2013Dawley rats. Our results revealed that stroke induced an imbalance in RAS pressor and depressor signaling, disrupted autophagic flux, and altered iron storage protein expression. These molecular abnormalities were accompanied by elevated blood pressure and suggest a mechanistic link to sympathetic dysregulation. Notably, transcranial direct current stimulation (tDCS) mitigated these stroke\u2010induced abnormalities by downregulating RAS pressor signaling, restoring impaired autophagic activity\u2014including autophagic ferritin degradation\u2014and consequently reducing iron accumulation\u2013associated oxidative stress in the RVLM. Collectively, our findings propose a putative molecular cascade contributing to post\u2010stroke sympathetic overactivity and highlight non\u2010invasive tDCS as a potential neuromodulatory intervention to restore neurochemical homeostasis in the RVLM.<\/jats:p>\n                  <jats:p>\n                    <jats:boxed-text content-type=\"graphic\" position=\"anchor\">\n                      <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc70464-gra-0001-m.png\">\n                        <jats:alt-text>image<\/jats:alt-text>\n                      <\/jats:graphic>\n                    <\/jats:boxed-text>\n                  <\/jats:p>","DOI":"10.1111\/jnc.70464","type":"journal-article","created":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T12:23:46Z","timestamp":1780057426000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Transcranial Direct Current Stimulation Attenuates Ischemic Stroke\u2010Induced Autonomic and Behavioral Dysfunction via a\n                    <scp>RAS<\/scp>\n                    \u2013Autophagy\u2013Iron Axis in the Brainstem"],"prefix":"10.1111","volume":"170","author":[{"given":"Yun","family":"Hsu","sequence":"first","affiliation":[{"name":"Institute of Basic Medical Sciences, College of Medicine National Cheng Kung University  Tainan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chou\u2010Ching","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Neurology, College of Medicine National Cheng Kung University  Tainan Taiwan"},{"name":"National Cheng Kung University Hospital  Tainan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chih\u2010Hsu","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Neurology, College of Medicine National Cheng Kung University  Tainan Taiwan"},{"name":"National Cheng Kung University Hospital  Tainan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-8682-7250","authenticated-orcid":false,"given":"Alice Y. 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