{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T02:26:00Z","timestamp":1792117560169,"version":"4.4.0"},"reference-count":51,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T00:00:00Z","timestamp":1782432000000},"content-version":"vor","delay-in-days":25,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100020598","name":"Multiple Sclerosis Society of Western Australia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100020598","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100019111","name":"Perron Institute for Neurological and Translational Science","doi-asserted-by":"publisher","award":["SA EUS 100a Fund"],"award-info":[{"award-number":["SA EUS 100a Fund"]}],"id":[{"id":"10.13039\/501100019111","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disorder characterised by progressive muscle weakness in both bulbar and extremity muscles, leading to a diverse clinical phenotype with motor and non\u2010motor symptoms. Approximately 85% of ALS cases are sporadic (sALS), while the remaining 10%\u201315% are familial (fALS). Biological biomarkers of sporadic ALS remain poorly understood, hindering precise patient screening, delaying diagnosis and negatively affecting prognosis. This study aims to identify potential proteomic biomarkers by comparing the cerebrospinal fluid (CSF) of sALS patients with that of patients suffering from other neurological diseases. Liquid chromatography\u2013tandem mass spectrometry (LC\u2013MS\/MS) was used for proteomic profiling of CSF samples from 24 sALS patients and 26 patients with other neurological diseases. The complete protein expression profiles were compared using a two\u2010tailed Student's\n                    <jats:italic>t<\/jats:italic>\n                    \u2010test, with a\n                    <jats:italic>p<\/jats:italic>\n                    &lt;\u20090.05 considered statistically significant with additional FDR correction at the 0.1 level. Proteomic analysis of CSF samples identified significant quantitative changes in 96 proteins with threshold\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05 and 74 proteins with FDR &lt;\u20090.1 between sALS and non\u2010ALS patients, including alterations in proteins associated with neurodegenerative processes, such as amyloid precursor proteins and inflammatory markers. CSF proteomic analysis reveals altered inflammatory and neurodegenerative metabolic pathways, providing valuable insights into the proteomic landscape of sALS. Several dysregulated proteins were consistent with the disease mechanisms highlighted in previous studies. These findings represent a step forward in developing personalised approaches for diagnosing and managing the disease.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:boxed-text content-type=\"graphic\" position=\"anchor\">\n                      <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc70508-gra-0001-m.png\">\n                        <jats:alt-text>image<\/jats:alt-text>\n                      <\/jats:graphic>\n                    <\/jats:boxed-text>\n                  <\/jats:p>","DOI":"10.1111\/jnc.70508","type":"journal-article","created":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T12:01:30Z","timestamp":1782475290000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Comparison of Proteomic Analysis of Cerebrospinal Fluid From Neurological Patients With and Without Amyotrophic Lateral Sclerosis"],"prefix":"10.1111","volume":"170","author":[{"given":"Eleonora","family":"Sabetta","sequence":"first","affiliation":[{"name":"IRCCS Ospedale San Raffaele  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karin","family":"Rallmann","sequence":"additional","affiliation":[{"name":"Department of Neurology Tartu University Hospital  Tartu Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5439-1022","authenticated-orcid":false,"given":"Pille","family":"Taba","sequence":"additional","affiliation":[{"name":"Department of Neurology Tartu University Hospital  Tartu Estonia"},{"name":"Institute of Clinical Medicine University Tartu  Tartu Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abigail L.","family":"Pfaff","sequence":"additional","affiliation":[{"name":"Perron Institute for Neurological and Translational Science  Perth Western Australia Australia"},{"name":"Personalised Medicine Center, Murdoch University, Western Australia Murdoch University  Perth Western Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bal Hari","family":"Poudel","sequence":"additional","affiliation":[{"name":"Personalised Medicine Center, Murdoch University, Western Australia Murdoch University  Perth Western Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Davide","family":"Ferrari","sequence":"additional","affiliation":[{"name":"SCVSA Department University of Parma  Parma Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Massimo","family":"Locatelli","sequence":"additional","affiliation":[{"name":"IRCCS Ospedale San Raffaele  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sulev","family":"K\u00f5ks","sequence":"additional","affiliation":[{"name":"Perron Institute for Neurological and Translational Science  Perth Western Australia Australia"},{"name":"Personalised Medicine Center, Murdoch University, Western Australia Murdoch University  Perth Western Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4597-041X","authenticated-orcid":false,"given":"Jonas","family":"Bergquist","sequence":"additional","affiliation":[{"name":"Analytical Chemistry and Neurochemistry, Department of Chemistry for Life Sciences, Biomedical Center Uppsala University  Uppsala Sweden"},{"name":"The OMF ME\/CFS Collaborative Research Center at Uppsala University Biomedical Center  Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,26]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-023-02611-y"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-34642-x"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00702-012-0801-3"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2015.00010"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/dds215"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/WCO.0000000000001279"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2010.04.004"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.09.037"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2022.838612"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1080\/21678421.2016.1245757"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(22)01272-7"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-12191-8"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms222212533"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(21)00465-8"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0b013e3182662031"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.26508\/lsa.201800070"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13024-022-00558-4"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp-2018-320151"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-022-02934-z"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40001-023-01596-4"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40478-022-01322-x"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1159\/000493386"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/JLB.3VMR0118-036R"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.17305\/bb.2024.11218"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.109.200451"},{"key":"e_1_2_12_27_1","doi-asserted-by":"crossref","unstructured":"Mamoor S.2022.\u201cDifferential Expression of CD163 in Amyotrophic Lateral Sclerosis.\u201dhttps:\/\/doi.org\/10.31219\/osf.io\/q7549.","DOI":"10.31219\/osf.io\/sz4fu"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/ene.14393"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1136\/jmedgenet-2020-106866"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.4081\/ni.2014.5367"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2020.113588"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1080\/21678421.2020.1779303"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/mds.28391"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms232214498"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-019-02093-x"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2019.104497"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23169299"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awad243"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp-2018-319210"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0010980"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI32723"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2008.197"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-16937-8"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2007.06.004"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12014-020-09294-7"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.1379\/CSC-126.1"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13024-025-00838-9"},{"key":"e_1_2_12_48_1","article-title":"Amyotrophic Lateral Sclerosis\u2010The Complex Phenotype\u2010From an Epidemiological Perspective: A Focus on Extrapyramidal and Non\u2010Motor Features","volume":"10","author":"Urso D.","year":"2022","journal-title":"Biomedicine"},{"key":"e_1_2_12_49_1","article-title":"The Need for Biomarkers in the ALS\u2010FTD Spectrum: A Clinical Point of View on the Role of Proteomics","volume":"11","author":"Vignaroli F.","year":"2023","journal-title":"PRO"},{"key":"e_1_2_12_50_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2017.00294"},{"key":"e_1_2_12_51_1","doi-asserted-by":"publisher","DOI":"10.4103\/2152-7806.169561"},{"key":"e_1_2_12_52_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12192-013-0427-x"}],"container-title":["Journal of Neurochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jnc.70508","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jnc.70508","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jnc.70508","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T07:10:43Z","timestamp":1790320243000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jnc.70508"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6]]},"references-count":51,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1111\/jnc.70508"],"URL":"https:\/\/doi.org\/10.1111\/jnc.70508","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1111\/JNC.70508\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JNC.70508\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JNC.70508\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JNC.70508\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JNC.70508\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JNC.70508\/v1\/decision1","asserted-by":"object"}]},"ISSN":["0022-3042","1471-4159"],"issn-type":[{"value":"0022-3042","type":"print"},{"value":"1471-4159","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6]]},"assertion":[{"value":"2025-07-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70508"}}