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Recently we reported that NEP trigger transient, voltage\u2010gated Ca\n                    <jats:sup>2+<\/jats:sup>\n                    channel\u2010mediated Ca\n                    <jats:sup>2+<\/jats:sup>\n                    influx in cultured murine adrenal chromaffin cells (ACC) expressing the genetically\u2010encoded Ca\n                    <jats:sup>2+<\/jats:sup>\n                    indicator GCaMP6f. The present study investigated ACC Ca\n                    <jats:sup>2+<\/jats:sup>\n                    responses to NEP in acute adrenal slices from mice that expressed GCaMP6f in both ACC and satellite glial cells (SGC), as well as exclusively in SGC. Adrenal glands from male and female mice were sectioned into 100\u2009\u03bcm slices that were placed in a chamber and perfused at a rate of 3\u20134\u2009mL\/min with a balanced salt solution maintained at 35\u00b0C. A custom\u2010fabricated non\u2010contact electrode delivered NEP ranging from 12 to 90\u2009ns to a large area of the adrenal medulla. Our results show that a single 30\u2009ns pulse elicited a rapid, transient rise in intracellular Ca\n                    <jats:sup>2+<\/jats:sup>\n                    in ACC in\u00a0situ. Like NEP\u2010induced Ca\n                    <jats:sup>2+<\/jats:sup>\n                    responses in cultured ACC, ACC Ca\n                    <jats:sup>2+<\/jats:sup>\n                    responses in\u00a0situ depended on extracellular Ca\n                    <jats:sup>2+<\/jats:sup>\n                    and voltage\u2010gated Ca\n                    <jats:sup>2+<\/jats:sup>\n                    channel activation. The nicotinic receptor antagonist hexamethonium failed to inhibit these responses, indicating that NEP stimulation, in contrast to stimulation with conventional duration electrical pulses, activates ACC directly rather than indirectly through activation of splanchnic nerve terminals. Furthermore, NEP exposure of the adrenal medulla also resulted in Ca\n                    <jats:sup>2+<\/jats:sup>\n                    increases in surrounding SGC that were slower in onset and longer in duration than those evoked by NEP in ACC. Finally, comparison of Ca\n                    <jats:sup>2+<\/jats:sup>\n                    responses elicited by NEP trains in ACC in\u00a0situ to those evoked by such trains in ACC in\u00a0vitro revealed that the native tissue environment promotes more highly synchronized Ca\n                    <jats:sup>2+<\/jats:sup>\n                    responses. Together, these results lay the foundation for future studies exploring the potential for NEP to modulate catecholamine release from ACC remotely and non\u2010invasively in\u00a0vivo.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:boxed-text content-type=\"graphic\" position=\"anchor\">\n                      <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc70525-gra-0001-m.png\">\n                        <jats:alt-text>image<\/jats:alt-text>\n                      <\/jats:graphic>\n                    <\/jats:boxed-text>\n                  <\/jats:p>","DOI":"10.1111\/jnc.70525","type":"journal-article","created":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T15:26:16Z","timestamp":1784733976000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Activation 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