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We have investigated the role of <jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>lk1 in pituitary development and function from late embryogenesis to adulthood using a mouse model completely lacking the expression of <jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>lk1. We confirm that <jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>lk1\u2010null mice are shorter and weigh less than wild\u2010type littermates from late gestation, at parturition and in adulthood. A loss of <jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>lk1 leads to significant reduction in <jats:styled-content style=\"fixed-case\">GH<\/jats:styled-content> content throughout life, whereas other pituitary hormones are reduced to varying degrees depending on sex and age. Both the size of the pituitary and the proportion of hormone\u2010producing cell populations are unchanged, suggesting that there is a reduction in hormone content per cell. <jats:italic>In vivo<\/jats:italic> challenge of mutant and wild\u2010type littermates with growth hormone\u2010releasing hormone and growth hormone\u2010releasing hexapeptide shows that reduced <jats:styled-content style=\"fixed-case\">GH<\/jats:styled-content> secretion is unlikely to account for the reduced growth of <jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>lk1 knockout animals. These data suggest that loss of Dlk1 gives rise to minor pituitary defects manifesting as an age\u2010 and sex\u2010dependent reduction in pituitary hormone contents. However, <jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>lk1 expression in other tissue is most likely responsible for the weight and length differences observed in mutant animals.<\/jats:p>","DOI":"10.1111\/jne.12010","type":"journal-article","created":{"date-parts":[[2012,12,21]],"date-time":"2012-12-21T20:28:22Z","timestamp":1356121702000},"page":"391-401","source":"Crossref","is-referenced-by-count":42,"title":["Pituitary Phenotypes of Mice Lacking the Notch Signalling Ligand Delta\u2010Like 1 Homologue"],"prefix":"10.1111","volume":"25","author":[{"given":"L. Y. 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