{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T10:16:23Z","timestamp":1792145783729,"version":"4.4.0"},"reference-count":75,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2013,9,12]],"date-time":"2013-09-12T00:00:00Z","timestamp":1378944000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National Institute of Health","award":["HD05751-39"],"award-info":[{"award-number":["HD05751-39"]}]},{"name":"Tri-institutional training fellowship","award":["5T32DK007313-33"],"award-info":[{"award-number":["5T32DK007313-33"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Neuroendocrinology"],"published-print":{"date-parts":[[2013,10]]},"abstract":"<jats:p>Expression of the primary female sex behaviour, lordosis, in laboratory animals depends on oestrogen\u2010induced expression of progesterone receptor (PgR) within a defined cell group in the ventrolateral portion of the ventromedial nucleus of the hypothalamus (<jats:styled-content style=\"fixed-case\">VMH<\/jats:styled-content>). The minimal latency from oestradiol administration to lordosis is 18\u00a0h. During that time, ligand\u2010bound oestrogen receptors (<jats:styled-content style=\"fixed-case\">ER<\/jats:styled-content>), members of a nuclear receptor superfamily, recruit transcriptional coregulators, which induce covalent modifications of histone proteins, thus leading to transcriptional activation or repression of target genes. The present study aimed to investigate the early molecular epigenetic events underlying oestrogen\u2010regulated transcriptional activation of the <jats:italic>Pgr<\/jats:italic> gene in the <jats:styled-content style=\"fixed-case\">VMH<\/jats:styled-content> of female mice. Oestradiol (E<jats:sub>2<\/jats:sub>) administration induced rapid and transient global histone modifications in the <jats:styled-content style=\"fixed-case\">VMH<\/jats:styled-content> of ovariectomised female mice. Histone H3\u00a0N\u2010terminus phosphorylation (H3S10phK14Ac), acetylation (H3Ac) and methylation (H3K4me3) exhibited distinct temporal patterns facilitative to the induction of transcription. A transcriptional repressive (H3K9me3) modification showed a different temporal pattern. Collectively, this should create a permissive environment for the transcriptional activity necessary for lordosis, within 3\u20136\u00a0h after E<jats:sub>2<\/jats:sub> treatment. In the <jats:styled-content style=\"fixed-case\">VMH<\/jats:styled-content>, changes in the H3Ac and H3K4me3 levels of histone H3 were also detected at the promoter region of the <jats:italic>Pgr<\/jats:italic> gene within the same time window, although they were delayed in the preoptic area. Moreover, examination of histone modifications associated with the promoter of another ER\u2010target gene, oxytocin receptor (<jats:italic>Oxtr<\/jats:italic>), revealed gene\u2010 and brain\u2010region specific effects of E<jats:sub>2<\/jats:sub> treatment. In the VMH of female mice, E<jats:sub>2<\/jats:sub> treatment resulted in the recruitment of ER\u03b1 to the oestrogen\u2010response\u2010elements\u2010containing putative enhancer site of <jats:italic>Pgr<\/jats:italic> gene, approximately 200\u00a0kb upstream of the transcription start site, although it failed to increase ER\u03b1 association with the more proximal promoter region. Finally, E<jats:sub>2<\/jats:sub> administration led to significant changes in the <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression of several <jats:styled-content style=\"fixed-case\">ER<\/jats:styled-content> coregulators in a brain\u2010region dependent manner. Taken together, these data indicate that, in the hypothalamus and preoptic area of female mice, early responses to E<jats:sub>2<\/jats:sub> treatment involve highly specific changes in chromatin structure, dependent on cell group, gene, histone modification studied, promoter\/enhancer site and time following E<jats:sub>2<\/jats:sub>.<\/jats:p>","DOI":"10.1111\/jne.12085","type":"journal-article","created":{"date-parts":[[2013,8,8]],"date-time":"2013-08-08T15:37:53Z","timestamp":1375976273000},"page":"939-955","source":"Crossref","is-referenced-by-count":36,"title":["Early Histone Modifications in the Ventromedial Hypothalamus and Preoptic Area Following Oestradiol Administration"],"prefix":"10.1111","volume":"25","author":[{"given":"K.","family":"Gagnidze","sequence":"first","affiliation":[{"name":"Laboratory of Neurobiology and Behaviour The Rockefeller University New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z. 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