{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T08:16:05Z","timestamp":1740125765018,"version":"3.37.3"},"reference-count":60,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2014,7,22]],"date-time":"2014-07-22T00:00:00Z","timestamp":1405987200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["24500411"],"award-info":[{"award-number":["24500411"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Neuroendocrinology"],"published-print":{"date-parts":[[2014,8]]},"abstract":"Notch signalling has a key role in cell fate specification in developing brains; however, recent studies have shown that Notch signalling also participates in the regulation of synaptic plasticity in adult brains. In the present study, we examined the expression of N<\/jats:styled-content>otch3 and D<\/jats:styled-content>elta\u2010like ligand 4 (DLL<\/jats:styled-content>4) in the hypothalamic\u2010neurohypophysial system (HNS<\/jats:styled-content>) of the adult mouse. The expression of DLL<\/jats:styled-content>4 was higher in the supraoptic nucleus (SON<\/jats:styled-content>) and paraventricular nucleus (PVN<\/jats:styled-content>) compared to adjacent hypothalamic regions. Double\u2010labelling immunohistochemistry using vesicular GABA<\/jats:styled-content> transporter and glutamate transporter revealed that DLL<\/jats:styled-content>4 was localised at a subpopulation of excitatory and inhibitory axonal boutons against somatodendrites of arginine vasopressin (AVP<\/jats:styled-content>)\u2010 and oxytocin (OXT<\/jats:styled-content>)\u2010containing magnocellular neurones. In the neurohypophysis (NH<\/jats:styled-content>), the expression of DLL<\/jats:styled-content>4 was seen at OXT<\/jats:styled-content>\u2010 but not AVP<\/jats:styled-content>\u2010containing axonal terminals. The expression of N<\/jats:styled-content>otch3 was seen at somatodendrites of AVP<\/jats:styled-content>\u2010 and OXT<\/jats:styled-content>\u2010containing magnocellular neurones in the SON<\/jats:styled-content> and PVN<\/jats:styled-content> and at pituicytes in the NH<\/jats:styled-content>. Chronic physiological stimulation by salt loading, which remarkably enhances the release of AVP<\/jats:styled-content> and OXT<\/jats:styled-content>, decreased the number of DLL<\/jats:styled-content>4\u2010immunoreactive axonal boutons in the SON<\/jats:styled-content> and PVN<\/jats:styled-content>. Moreover, chronic and acute osmotic stimulation promoted proteolytic cleavage of N<\/jats:styled-content>otch3 to yield the intracellular fragments of N<\/jats:styled-content>otch3 in the HNS<\/jats:styled-content>. Thus, the present study demonstrates activity\u2010dependent reduction of DLL<\/jats:styled-content>4 expression and proteolytic cleavage of N<\/jats:styled-content>otch3 in the HNS<\/jats:styled-content>, suggesting that N<\/jats:styled-content>otch signalling possibly participates in synaptic interaction in the hypothalamic nuclei and neuroglial interaction in the NH<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/jne.12172","type":"journal-article","created":{"date-parts":[[2014,6,18]],"date-time":"2014-06-18T15:56:41Z","timestamp":1403107001000},"page":"497-509","source":"Crossref","is-referenced-by-count":5,"title":["Activity\u2010Dependent Notch Signalling in the Hypothalamic\u2010Neurohypophysial System of Adult Mouse Brains"],"prefix":"10.1111","volume":"26","author":[{"given":"T.","family":"Mannari","sequence":"first","affiliation":[{"name":"Department of Applied Biology Kyoto Institute of Technology Kyoto 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