{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T03:37:03Z","timestamp":1761709023479},"reference-count":56,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2014,11,17]],"date-time":"2014-11-17T00:00:00Z","timestamp":1416182400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Canadian NSERC"},{"name":"Dreamworld"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Neuroendocrinology"],"published-print":{"date-parts":[[2014,12]]},"abstract":"<jats:p>During evolution, reproductive hormones and their receptors in the brain\u2010pituitary\u2010gonadal axis have been altered by genetic mechanisms. To understand how the neuroendocrine control of reproduction evolved in mammals, it is important to examine marsupials, the closest group to placental mammals. We hypothesised that at least some of the hormones and receptors found in placental mammals would be present in koala, a marsupial. We examined the expression of koala m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> for the reproductive molecules. Koala c<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>s were cloned from brain for gonadotrophin\u2010releasing hormones (<jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>n<jats:styled-content style=\"fixed-case\">RH<\/jats:styled-content>1 and <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>n<jats:styled-content style=\"fixed-case\">RH<\/jats:styled-content>2) or from pituitary for <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>n<jats:styled-content style=\"fixed-case\">RH<\/jats:styled-content> receptors, types <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content>, follicle\u2010stimulating hormone (<jats:styled-content style=\"fixed-case\">FSH)<\/jats:styled-content>\u03b2 and luteinising hormone (<jats:styled-content style=\"fixed-case\">LH)<\/jats:styled-content>\u03b2, and from gonads for <jats:styled-content style=\"fixed-case\">FSH<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">LH<\/jats:styled-content> receptors. Deduced proteins were compared by sequence alignment and phylogenetic analysis with those of other vertebrates. In conclusion, the koala expressed m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> for these eight putative reproductive molecules, whereas at least one of these molecules is missing in some species in the amniote lineage, including humans. In addition, <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>n<jats:styled-content style=\"fixed-case\">RH<\/jats:styled-content>1 and 2 are shown by immunohistochemistry to be expressed as proteins in the brain.<\/jats:p>","DOI":"10.1111\/jne.12208","type":"journal-article","created":{"date-parts":[[2014,9,8]],"date-time":"2014-09-08T15:53:04Z","timestamp":1410191584000},"page":"870-887","source":"Crossref","is-referenced-by-count":7,"title":["Molecular Analysis of the Koala Reproductive Hormones and Their Receptors: Gonadotrophin\u2010Releasing Hormone (GnRH),<scp> Follicle\u2010Stimulating Hormone <\/scp>\u03b2 and <scp>Luteinising Hormone <\/scp>\u03b2 with Localisation of <scp>G<\/scp>n<scp>RH<\/scp>"],"prefix":"10.1111","volume":"26","author":[{"given":"E. R.","family":"Busby","sequence":"first","affiliation":[{"name":"Department of Biology University of Victoria Victoria B.C. Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Soeta","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine Nippon Veterinary and Life Science University Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. M.","family":"Sherwood","sequence":"additional","affiliation":[{"name":"Department of Biology University of Victoria Victoria B.C. Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. D.","family":"Johnston","sequence":"additional","affiliation":[{"name":"School of Agriculture and Food Science The University of Queensland Gatton Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,11,17]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"42","volume-title":"Fauna of Australia. Vol. 1B, Mammalia","author":"Dawson TJ","year":"1989"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05634"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06936"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2011-12-8-r81"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.107.064824"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD07214"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2010.12.014"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06967"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-0115(91)90206-V"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(03)00758-3"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1677\/JME-06-0010"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1006\/gcen.1997.6970"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10390"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2014.07.025"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2007.01.030"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05805"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1102838108"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1159\/000233303"},{"key":"e_1_2_7_20_1","doi-asserted-by":"crossref","first-page":"1538","DOI":"10.1210\/mend.16.7.0871","article-title":"Evolution of glycoproteins hormone subunit genes in bilateral metazoa: identification of two novel human glycoprotein hormone subunit family genes, GPA2 and GPB5","volume":"16","author":"Hsu SY","year":"2002","journal-title":"Mol Endocrinol"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2005-0317"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2148-11-332"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI0214340"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1385\/ENDO:26:3:267"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2008-1743"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/gbe\/evu118"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-012515400-0\/50035-X"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD01124"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1006\/gcen.1999.7418"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2010.10.024"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-125-5-2244"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1071\/R97004"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1006\/gcen.1996.6847"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1987.tb36299.x"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.0770347"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4320(98)00117-1"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00299-001-0399-7"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.2006.00060.x"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msp259"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2148-10-210"},{"key":"e_1_2_7_41_1","first-page":"164","article-title":"PHYLIP \u2013 phylogeny inference package (version 3.2)","volume":"5","author":"Felsenstein J","year":"1989","journal-title":"Cladistics"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1080\/10635150600755453"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.138.12.5592"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1159\/000125033"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.14.6373"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.1.305"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2005.10.004"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471733849"},{"key":"e_1_2_7_49_1","first-page":"3","volume-title":"GnRH Neurons: Gene to Behavior","author":"Sherwood NM","year":"1997"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.2003-0002"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2010.10.018"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.2568"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2011.11.004"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2004.01.006"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature03206"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1002\/prot.21937"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.10161"}],"container-title":["Journal of Neuroendocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjne.12208","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjne.12208","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jne.12208","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T12:23:10Z","timestamp":1696162990000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jne.12208"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,11,17]]},"references-count":56,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2014,12]]}},"alternative-id":["10.1111\/jne.12208"],"URL":"https:\/\/doi.org\/10.1111\/jne.12208","archive":["Portico"],"relation":{},"ISSN":["0953-8194","1365-2826"],"issn-type":[{"value":"0953-8194","type":"print"},{"value":"1365-2826","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,11,17]]}}}