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However, the functions of kisspeptin neurones in non\u2010mammalian vertebrates are not well understood. We have used transgenics to labell kisspeptin neurones (Kiss1 and Kiss2) with mCherry in zebrafish (<jats:italic>Danio rerio<\/jats:italic>). In <jats:italic>kiss1:mCherry<\/jats:italic> transgenic zebrafish, Kiss1 cells were located in the dorsomedial and ventromedial habenula, with their nerve fibres contributing to the fasciculus retroflexus and projecting to the ventral parts of the interpeduncular and raphe nuclei. In <jats:italic>kiss2:mCherry<\/jats:italic> zebrafish, Kiss2 cells were primarily located in the dorsal zone of the periventricular hypothalamus and, to a lesser extent, in the periventricular nucleus of the posterior tuberculum and the preoptic area. Kiss2 fibres formed a wide network projecting into the telencephalon, the mesencephalon, the hypothalamus and the pituitary. To study the relationship of kisspeptin neurones and Gn<jats:styled-content style=\"fixed-case\">RH<\/jats:styled-content>3 neurones, these fish were crossed with <jats:italic>gnrh3:<jats:styled-content style=\"fixed-case\">EGFP<\/jats:styled-content><\/jats:italic> zebrafish to obtain <jats:italic>kiss1:mCherry<\/jats:italic>\/<jats:italic>gnrh3:<jats:styled-content style=\"fixed-case\">EGFP<\/jats:styled-content><\/jats:italic> and <jats:italic>kiss2:mCherry<\/jats:italic>\/<jats:italic>gnrh3:<jats:styled-content style=\"fixed-case\">EGFP<\/jats:styled-content><\/jats:italic> double transgenic zebrafish. The Gn<jats:styled-content style=\"fixed-case\">RH<\/jats:styled-content>3 fibres ascending to the habenula were closely associated with Kiss1 fibres projecting from the ventral habenula. On the other hand, Gn<jats:styled-content style=\"fixed-case\">RH<\/jats:styled-content>3 fibres and Kiss2 fibres were adjacent but scarcely in contact with each other in the telencephalon and the hypothalamus. The Kiss2 and Gn<jats:styled-content style=\"fixed-case\">RH<\/jats:styled-content>3 fibres in the ventral hypothalamus projected into the pituitary via the pituitary stalk. In the pituitary, Kiss2 fibres were directly in contact with Gn<jats:styled-content style=\"fixed-case\">RH<\/jats:styled-content>3 fibres in the pars distalis. These results reveal the pattern of kisspeptin neurones and their connections with Gn<jats:styled-content style=\"fixed-case\">RH<\/jats:styled-content>3 neurones in the brain, suggesting distinct mechanisms for Kiss1 and Kiss2 in regulating reproductive events in zebrafish.<\/jats:p>","DOI":"10.1111\/jne.12251","type":"journal-article","created":{"date-parts":[[2014,12,20]],"date-time":"2014-12-20T06:23:41Z","timestamp":1419056621000},"page":"198-211","source":"Crossref","is-referenced-by-count":45,"title":["The Distribution of Kisspeptin (Kiss)1\u2010 and Kiss2\u2010Positive Neurones and Their Connections with Gonadotrophin\u2010Releasing Hormone\u20103 Neurones in the Zebrafish Brain"],"prefix":"10.1111","volume":"27","author":[{"given":"Y.","family":"Song","sequence":"first","affiliation":[{"name":"State Key Laboratory of Freshwater Ecology and Biotechnology Institute of Hydrobiology Chinese Academy of Sciences Wuhan China"},{"name":"University of Chinese Academy of Sciences 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