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In the anterior pituitary, folliculostellate (FS<\/jats:styled-content>) cells constitute the major source of bFGF<\/jats:styled-content>. bFGF<\/jats:styled-content> affects endocrine cell proliferation and secretion in the anterior pituitary. In addition, bFGF<\/jats:styled-content> increases its own expression by acting directly on FS<\/jats:styled-content> cells. FS<\/jats:styled-content> cell C<\/jats:styled-content>x43\u2010mediated gap junction intercellular communication allows the establishment of an intrapituitary network for the transmission of information. In the present study, we assessed how bFGF<\/jats:styled-content> regulates FS<\/jats:styled-content> cell coupling. Time course studies were carried out on the FS<\/jats:styled-content> cell line T<\/jats:styled-content>tT<\/jats:styled-content>\/GF<\/jats:styled-content>. Short\u2010term bFGF<\/jats:styled-content> treatment induced a transient cell uncoupling and the phosphorylation in S<\/jats:styled-content>er368 of membrane\u2010bound C<\/jats:styled-content>x43 without modifying C<\/jats:styled-content>x43 levels. We demonstrated the involvement of the protein kinase C (PKC)<\/jats:styled-content> isoform \u03b1 in the phosphorylation of C<\/jats:styled-content>x43 in S<\/jats:styled-content>368. Moreover, we showed that bFGF<\/jats:styled-content> induced PKC<\/jats:styled-content>\u03b1 activation by stimulating its expression, phosphorylation and association with the plasma membrane. The long\u2010term incubation with bFGF<\/jats:styled-content> increased T<\/jats:styled-content>tT<\/jats:styled-content>\/GF<\/jats:styled-content> cell coupling, total C<\/jats:styled-content>x43 levels and C<\/jats:styled-content>x43 accumulation at the cell membrane of cytoplasmic projections. The C<\/jats:styled-content>x43 level increase was a result of the stimulation of C<\/jats:styled-content>x43 gene transcription as mediated by the extracellular\u2010regulated kinase 1\/2 signalling pathway. Taken together, the data show that bFGF<\/jats:styled-content> modulates T<\/jats:styled-content>tT<\/jats:styled-content>\/GF<\/jats:styled-content> cell coupling by activating different pathways that lead to opposite effects on C<\/jats:styled-content>x43 phosphorylation and expression depending on the duration of the exposure of the cells to bFGF<\/jats:styled-content>. A short\u2010term bFGF<\/jats:styled-content> exposure reduces cell\u2010to\u2010cell communication as a mean of desynchronising FS<\/jats:styled-content> cells. By contrast, long\u2010term exposure to bFGF<\/jats:styled-content> enhances cell\u2010to\u2010cell communication and facilitates coordination among FS<\/jats:styled-content> cells.<\/jats:p>","DOI":"10.1111\/jne.12308","type":"journal-article","created":{"date-parts":[[2015,8,12]],"date-time":"2015-08-12T08:25:29Z","timestamp":1439367929000},"page":"787-801","source":"Crossref","is-referenced-by-count":9,"title":["Biphasic Effect of Basic Fibroblast Growth Factor on Anterior Pituitary Folliculostellate TtT\/GF Cell Coupling, and Connexin 43 Expression and Phosphorylation"],"prefix":"10.1111","volume":"27","author":[{"given":"M. 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