{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:13:44Z","timestamp":1789517624001,"version":"build-2803163510"},"reference-count":35,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2015,10,28]],"date-time":"2015-10-28T00:00:00Z","timestamp":1445990400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001677","name":"Institut national de la sant\u00e9 et de la recherche m\u00e9dicale","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001677","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Neuroendocrinology"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:p>Brain\u2010derived neurotrophic factor (<jats:styled-content style=\"fixed-case\">BDNF<\/jats:styled-content>) appears to be highly involved in hypothalamic\u2010pituitary\u2010adrenal (<jats:styled-content style=\"fixed-case\">HPA<\/jats:styled-content>) axis regulation during adulthood, playing an important role in homeostasis maintenance. The present study aimed to determine the involvement of BDNF in <jats:styled-content style=\"fixed-case\">HPA<\/jats:styled-content> axis activity under basal and stress conditions via partial inhibition of this endogenous neurotrophin. Experiments were conducted in rats and mice with two complementary approaches: (i) <jats:styled-content style=\"fixed-case\">BDNF<\/jats:styled-content> knockdown with stereotaxic delivery of <jats:styled-content style=\"fixed-case\">BDNF<\/jats:styled-content>\u2010specific small interfering <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> (si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>) into the lateral ventricle of adult male rats and (ii) genetically induced knockdown (<jats:styled-content style=\"fixed-case\">KD<\/jats:styled-content>) of <jats:styled-content style=\"fixed-case\">BDNF<\/jats:styled-content> expression specifically in the central nervous system during the first ontogenesis in mice (<jats:styled-content style=\"fixed-case\">KD<\/jats:styled-content> mice). Delivery of si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> in the rat brain decreased <jats:styled-content style=\"fixed-case\">BDNF<\/jats:styled-content> levels in the hippocampus (\u221231%) and hypothalamus (\u221235%) but not in the amygdala, frontal cortex and pituitary. In addition, si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> induced no change of the basal <jats:styled-content style=\"fixed-case\">HPA<\/jats:styled-content> axis activity. <jats:styled-content style=\"fixed-case\">BDNF<\/jats:styled-content> si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> rats exhibited decreased <jats:styled-content style=\"fixed-case\">BDNF<\/jats:styled-content> levels and concomitant altered adrenocortoctrophic hormone (<jats:styled-content style=\"fixed-case\">ACTH)<\/jats:styled-content> and corticosterone responses to restraint stress, suggesting the involvement of <jats:styled-content style=\"fixed-case\">BDNF<\/jats:styled-content> in the <jats:styled-content style=\"fixed-case\">HPA<\/jats:styled-content> axis adaptive response to stress. In <jats:styled-content style=\"fixed-case\">KD<\/jats:styled-content> mice, <jats:styled-content style=\"fixed-case\">BDNF<\/jats:styled-content> levels in the hippocampus and hypothalamus were decreased by 20% in heterozygous and by 60% in homozygous animals compared to wild\u2010type littermates. Although, in heterozygous <jats:styled-content style=\"fixed-case\">KD<\/jats:styled-content> mice, no significant change was observed in the basal levels of plasma <jats:styled-content style=\"fixed-case\">ACTH<\/jats:styled-content> and corticosterone, both hormones were significantly increased in homozygous <jats:styled-content style=\"fixed-case\">KD<\/jats:styled-content> mice, demonstrating that robust cerebral <jats:styled-content style=\"fixed-case\">BDNF<\/jats:styled-content> inhibition (60%) is necessary to affect basal <jats:styled-content style=\"fixed-case\">HPA<\/jats:styled-content> axis activity. All of these results in both rats and mice demonstrate the involvement and importance of a robust endogenous pool of <jats:styled-content style=\"fixed-case\">BDNF<\/jats:styled-content> in basal <jats:styled-content style=\"fixed-case\">HPA<\/jats:styled-content> axis regulation and the pivotal function of <jats:italic>de novo <\/jats:italic><jats:styled-content style=\"fixed-case\">BDNF<\/jats:styled-content> synthesis in the establishment of an adapted response to stress.<\/jats:p>","DOI":"10.1111\/jne.12324","type":"journal-article","created":{"date-parts":[[2015,9,21]],"date-time":"2015-09-21T09:51:51Z","timestamp":1442829111000},"page":"850-860","source":"Crossref","is-referenced-by-count":29,"title":["Involvement of Endogenous Brain\u2010Derived Neurotrophic Factor in Hypothalamic\u2010Pituitary\u2010Adrenal Axis Activity"],"prefix":"10.1111","volume":"27","author":[{"given":"G.","family":"Naert","sequence":"first","affiliation":[{"name":"Molecular Mechanisms in 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