{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T19:03:44Z","timestamp":1768071824404,"version":"3.49.0"},"reference-count":69,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2016,5,11]],"date-time":"2016-05-11T00:00:00Z","timestamp":1462924800000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Neuroendocrinology"],"published-print":{"date-parts":[[2016,5]]},"abstract":"Chicken lines that have been divergently selected for either low (LWS<\/jats:styled-content>) or high (HWS<\/jats:styled-content>) body weight at 56\u00a0days of age for more than 57 generations have different feeding behaviours in response to a range of i.c.v. injected neurotransmitters. The LWS<\/jats:styled-content> have different severities of anorexia, whereas the HWS<\/jats:styled-content> become obese. Previously, we demonstrated that LWS<\/jats:styled-content> chicks did not respond, whereas HWS<\/jats:styled-content> chicks increased food intake, after central injection of neuropeptide Y (NPY<\/jats:styled-content>). The present study aimed to determine the molecular mechanisms underlying the loss of orexigenic function of NPY<\/jats:styled-content> in LWS<\/jats:styled-content>. Chicks were divided into four groups: stressed LWS<\/jats:styled-content> and HWS<\/jats:styled-content> on day of hatch, and control LWS<\/jats:styled-content> and HWS<\/jats:styled-content>. The stressor was a combination of food deprivation and cold exposure. On day 5 post\u2010hatch, each chick received an i.c.v.<\/jats:styled-content> injection of vehicle or 0.2\u00a0nmol of NPY<\/jats:styled-content>. Only the LWS<\/jats:styled-content> stressed group did not increase food intake in response to i.c.v. NPY<\/jats:styled-content>. Hypothalamic mRNA<\/jats:styled-content> abundance of appetite\u2010associated factors was measured at 1\u00a0h post\u2010injection. Interactions of genetic line, stress and NPY<\/jats:styled-content> treatment were observed for the mRNA<\/jats:styled-content> abundance of agouti\u2010related peptide (AgRP<\/jats:styled-content>) and synaptotagmin 1 (SYT<\/jats:styled-content>1). Intracerebroventricular<\/jats:styled-content> injection of NPY<\/jats:styled-content> decreased and increased AgRP<\/jats:styled-content> and SYT<\/jats:styled-content>1 mRNA<\/jats:styled-content>, respectively, in the stressed LWS<\/jats:styled-content> and increased AgRP mRNA<\/jats:styled-content> in stressed HWS<\/jats:styled-content> chicks. Stress was associated with increased NPY<\/jats:styled-content>, orexin receptor 2, corticotrophin\u2010releasing factor receptor 1, melanocortin receptor 3 (MC<\/jats:styled-content>3R) and growth hormone secretagogue receptor expression. In conclusion, the loss of responsiveness to exogenous NPY<\/jats:styled-content> in stressed LWS<\/jats:styled-content> chicks may be a result of the decreased and increased hypothalamic expression of AgRP<\/jats:styled-content> and MC<\/jats:styled-content>3R, respectively. This may induce an intensification of anorexigenic melanocortin signalling pathways in LWS<\/jats:styled-content> chicks that block the orexigenic effect of exogenous NPY<\/jats:styled-content>. These results provide insights onto the anorexic condition across species, and especially for forms of inducible anorexia such as human anorexia nervosa.<\/jats:p>","DOI":"10.1111\/jne.12378","type":"journal-article","created":{"date-parts":[[2016,2,29]],"date-time":"2016-02-29T12:19:46Z","timestamp":1456748386000},"source":"Crossref","is-referenced-by-count":14,"title":["Anorexia is Associated with Stress\u2010Dependent Orexigenic Responses to Exogenous Neuropeptide Y"],"prefix":"10.1111","volume":"28","author":[{"given":"J.","family":"Yi","sequence":"first","affiliation":[{"name":"Department of Animal and Poultry Sciences Virginia Polytechnic Institute and State University Blacksburg VA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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